QIU Zhiquan, CAO Qingqing, TAN Weifeng, ZHOU Jin, LÜ Lei. Assay of eupatilin and arteanoflavone in Artemisia anomala by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(2): 163-166. doi: 10.3969/j.issn.1006-0111.2016.02.016
| Citation:
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QIU Zhiquan, CAO Qingqing, TAN Weifeng, ZHOU Jin, LÜ Lei. Assay of eupatilin and arteanoflavone in Artemisia anomala by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(2): 163-166. doi: 10.3969/j.issn.1006-0111.2016.02.016
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Assay of eupatilin and arteanoflavone in Artemisia anomala by HPLC
- 1.
Derpartment of Biliary Tract Surgery, Eastern Hepatobiliary Hospital Affiliated to Second Military Medical University, Shanghai 200438, China
- 2.
Derpartment of Pharmacy, Eastern Hepatobiliary Hospital Affiliated to Second Military Medical University, Shanghai 200438, China
- Received Date: 2015-02-10
- Rev Recd Date:
2015-06-08
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Abstract
Objective To determine the concentration of eupatilin and arteanoflavone in Artemisia anomala by high performance liquid chromatography(HPLC). Methods Artemisia anomala was extracted by ultrasonic for 60 minutes with 10 times volume of methanol. The HPLC was performed on a SHISEIDO MG-C18 column(3.0 mm×100 mm, 3μm). The mobile phase was a mixture of acetonitrile(ACN) and 0.1% formic acid(40:60, V/V). The detection wavelength was 350 nm, the column temperature was 25℃ and the injection volumn was 5μl. Results Eupatilin and arteanoflavone were separated at baseline within 15min with good linearity. The method validation results show that the precisions, repeatability and stability were all in the normal range. The low,medium and high level recoveries of eupatilin were 100.26%,99.58%,102.24%,and those of arteanoflavone were 99.09%,101.12%,101.43%,respectively. Conclusion The method was rapid, simple, reproductive and accurate. It can be used to control the quality of Artemisia anomala.
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