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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2016  >  34(1): 59-61

GAO Liping, LI Ping, YAO Jiajia. Determination of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(1): 59-61. doi: 10.3969/j.issn.1006-0111.2016.01.016
Citation: GAO Liping, LI Ping, YAO Jiajia. Determination of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(1): 59-61. doi: 10.3969/j.issn.1006-0111.2016.01.016
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Determination of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC

doi: 10.3969/j.issn.1006-0111.2016.01.016

  • Shanghai Xinxing Medicine Co., Ltd, Shanghai 200135, China

  • Received Date: 2015-06-20
  • Rev Recd Date: 2015-10-30
  • Objective To establish the method for determination content of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC. Methods The analysis was carried on a Shim-Pack CLC-ODS column with a mobile phase of 50% acetonitrile -0.05 mol/L sodium acetate buffer (35:65) at the detection wavelength of 360 nm, using alanine and 2,4-dinitrofluorobenzene as the internal standard and derivation agent, respectively. Results The method showed a good linearity in the range of 0.006-0.030 mg/ml (r=0.999 3) for glycine. The average recovery was 101.4%, and the RSD was 0.14% (n=9). Conclusion This method was simple, sensitive, accurate, reliable, and suitable for determination of glycine in the pharmaceutical of human coagulation factor Ⅷ.
  • [1] Schwarzinger I,Pabinger I,Kominger C,et al.Incidence of inhibitors in patients with severe and moderate hemophilia a treated with factor Ⅷ concentrates[J].Am J Hematol,2006,24(3):241-245.
    [2] Van DK, Van Der Bom JG, Lenting PJ, et al. Facter Ⅷ half-life and clinical phenotype of sever hemophilia A[J]. Haematologica, 2005,90(4):494-498.
    [3] 赵艳华, 马 平, 卜凤荣. 凝血因子分子生物学研究进展[J]. 生物技术通讯,1999,10(2):158-160.
    [4] 孙东坡,胡一桥.蛋白质冷冻干燥制品中的保护剂及其保护机制[J].药学进展,2003,27(4):202-205.
    [5] 李 瑜,江 勇,李 爽.反相高效液相色谱法测定发酵液中L-精氨酸含量[J]. 工业微生物,2004,34(3):32-34.
    [6] 尉小平,冉铁成,王 坚,等.柱前衍生反相高效液相色谱法测定冻干人纤维蛋白原制品中盐酸精氨酸和甘氨酸的含量[J]. 中国生物制品学杂志,2007,20(8):609-611.
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Determination of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC

doi: 10.3969/j.issn.1006-0111.2016.01.016
  • Shanghai Xinxing Medicine Co., Ltd, Shanghai 200135, China
  • Received Date: 2015-06-20
  • Rev Recd Date: 2015-10-30

Abstract: Objective To establish the method for determination content of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC. Methods The analysis was carried on a Shim-Pack CLC-ODS column with a mobile phase of 50% acetonitrile -0.05 mol/L sodium acetate buffer (35:65) at the detection wavelength of 360 nm, using alanine and 2,4-dinitrofluorobenzene as the internal standard and derivation agent, respectively. Results The method showed a good linearity in the range of 0.006-0.030 mg/ml (r=0.999 3) for glycine. The average recovery was 101.4%, and the RSD was 0.14% (n=9). Conclusion This method was simple, sensitive, accurate, reliable, and suitable for determination of glycine in the pharmaceutical of human coagulation factor Ⅷ.

GAO Liping, LI Ping, YAO Jiajia. Determination of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(1): 59-61. doi: 10.3969/j.issn.1006-0111.2016.01.016
Citation: GAO Liping, LI Ping, YAO Jiajia. Determination of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(1): 59-61. doi: 10.3969/j.issn.1006-0111.2016.01.016
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