Determination and clinical pharmacy application of quetiapine fumarate in human serum by RP-HPLC

ZHANG Qiaozhen; SONG Weiming; WU Liusong

doi:10.3969/j.issn.1006-0111.2016.01.013

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Objective To establish a method for the determination of quetiapine fumarate in human serum by RP-HPLC and apply it into clinical. Methods Extracting with ethyl ether after serum-drug was alkalized, and then determined by RP-HPLC. The determination was performed on Zorbax Eclipse XDB-C₁₈ column with mobile phase consisted of methanol-water (70:30, containing 0.5% triethylamine and 0.4% glacial acetic acid) at the flow rate of 0.6 ml/min. The detection wavelength was set at 254 nm, and the column temperature was 35 ℃. The method would be applied into analysis of clinical medication. Results Quetiapine fumarate and the impurities could be completely separated, and the linear range of quetiapine fumarate were 50-1 000 ng/ml(r=0.999 5).The recovery of the method was 98.2%-100.1% and the recovery of extracting was 75.2%-84.6%. RSD of intra-day was within 0.8%-3.7% and RSD of inter-day was within 1.4%-5.1%.The limit of quantitation for quetiapine fumarate was 2.1 ng/ml.This method had been applied into clinical pharmacy and achieved a good effects. Conclusions The method is simple, accurate, reproducible, and sensitive for determination of quetiapine fumarate in human serum. It has important significance on instructing the rational use of clinical medicine and discovering the unreasonable drug combination.

Determination and clinical pharmacy application of quetiapine fumarate in human serum by RP-HPLC

Kangning Hospital, Ningbo 315201, China

Received Date: 2014-12-05

Rev Recd Date: 2015-04-01

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Abstract: Objective To establish a method for the determination of quetiapine fumarate in human serum by RP-HPLC and apply it into clinical. Methods Extracting with ethyl ether after serum-drug was alkalized, and then determined by RP-HPLC. The determination was performed on Zorbax Eclipse XDB-C₁₈ column with mobile phase consisted of methanol-water (70:30, containing 0.5% triethylamine and 0.4% glacial acetic acid) at the flow rate of 0.6 ml/min. The detection wavelength was set at 254 nm, and the column temperature was 35 ℃. The method would be applied into analysis of clinical medication. Results Quetiapine fumarate and the impurities could be completely separated, and the linear range of quetiapine fumarate were 50-1 000 ng/ml(r=0.999 5).The recovery of the method was 98.2%-100.1% and the recovery of extracting was 75.2%-84.6%. RSD of intra-day was within 0.8%-3.7% and RSD of inter-day was within 1.4%-5.1%.The limit of quantitation for quetiapine fumarate was 2.1 ng/ml.This method had been applied into clinical pharmacy and achieved a good effects. Conclusions The method is simple, accurate, reproducible, and sensitive for determination of quetiapine fumarate in human serum. It has important significance on instructing the rational use of clinical medicine and discovering the unreasonable drug combination.

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Reference (14)

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Determination and clinical pharmacy application of quetiapine fumarate in human serum by RP-HPLC

doi: 10.3969/j.issn.1006-0111.2016.01.013

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