Preparation of DNA-loaded chitosan nanoparticle vaccine

XIANG Jingjie; ZHONG Yanqiang; LU Yiming; LU Ying

doi:10.3969/j.issn.1006-0111.2016.01.006

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Objective To study and optimize the preparation condition of pVAX1-wapA-loaded nanoparticles and determine the transfection efficiency. Methods The related effects of the crucial factors for the formation of nanoparticles: concentration of chitosan and TPP, pH value, N/P ratio were studied by single-factor experiment, with nanoparticles size and zeta potential as index. Cell transfection test was carried out to indicate that enhancement of cell transfection efficiency of nano-carrier. Results Nanoparticles loaded DNA vaccine were nearly spherical shape with uniform particle size chitosan nanoparticle(CS),(219.2±18.2) nm;quaternary ammonium chitosan nanoparticles(CSTM),(222.5±15.6) nm. Zeta potential of CS and CSTM was (24.7±3.5) mV, (19.6±1.2) mV and encapsulation efficiency was 91.24%, 87.66%,respectively.CSTM nanoparticle could enhance cellular uptake of pVAX1-wapA obviously. Conclusion CSTM nanoparticle was proved to be an efficient DNA vaccine delivery vector.

Preparation of DNA-loaded chitosan nanoparticle vaccine

School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received Date: 2015-08-24

Rev Recd Date: 2015-11-16

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Abstract: Objective To study and optimize the preparation condition of pVAX1-wapA-loaded nanoparticles and determine the transfection efficiency. Methods The related effects of the crucial factors for the formation of nanoparticles: concentration of chitosan and TPP, pH value, N/P ratio were studied by single-factor experiment, with nanoparticles size and zeta potential as index. Cell transfection test was carried out to indicate that enhancement of cell transfection efficiency of nano-carrier. Results Nanoparticles loaded DNA vaccine were nearly spherical shape with uniform particle size chitosan nanoparticle(CS),(219.2±18.2) nm;quaternary ammonium chitosan nanoparticles(CSTM),(222.5±15.6) nm. Zeta potential of CS and CSTM was (24.7±3.5) mV, (19.6±1.2) mV and encapsulation efficiency was 91.24%, 87.66%,respectively.CSTM nanoparticle could enhance cellular uptake of pVAX1-wapA obviously. Conclusion CSTM nanoparticle was proved to be an efficient DNA vaccine delivery vector.

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Preparation of DNA-loaded chitosan nanoparticle vaccine

doi: 10.3969/j.issn.1006-0111.2016.01.006

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