Determination of the rosmarinic acid in Xiaokang capsule by HPLC

XIAO Jinbao; JIANG Zongwen; SUN Dan; XIAO Qiusheng; WANG Shuang

doi:10.3969/j.issn.1006-0111.2015.06.020

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Objective To develop a method for determination of the rosmarinic acid in Xiaokang capsules. Methods An HPLC method was developed. The determination was performed on Agilent C₁₈ column at 30℃ with a mobile phase composed of methanol: 0.5% formic acid (50:50) run at a flow rate of 1.0 ml/min. The detection wavelength was set at 330 nm and the injection volume was 10 μl. Results The linear range of rosmarinic acid concentrations was 2.88 to 20.1 μg/ml (r=0.999 8). The average recovery was 99.5% (RSD=0.40%,r=9). Conclusion An assay for the determination of the rosmarinic acid in Xiaokang capsule that is simple, rapid, accurate and reliable has been developed.

Determination of the rosmarinic acid in Xiaokang capsule by HPLC

1. Department of Pharmacy, 461th Clinical Department, No.208 Hospital of PLA, Changchun 130021, China

2. Outpatient Department of Air force Aviation University, Changchun 130021, China

Received Date: 2014-11-25

Rev Recd Date: 2015-04-03

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Abstract: Objective To develop a method for determination of the rosmarinic acid in Xiaokang capsules. Methods An HPLC method was developed. The determination was performed on Agilent C₁₈ column at 30℃ with a mobile phase composed of methanol: 0.5% formic acid (50:50) run at a flow rate of 1.0 ml/min. The detection wavelength was set at 330 nm and the injection volume was 10 μl. Results The linear range of rosmarinic acid concentrations was 2.88 to 20.1 μg/ml (r=0.999 8). The average recovery was 99.5% (RSD=0.40%,r=9). Conclusion An assay for the determination of the rosmarinic acid in Xiaokang capsule that is simple, rapid, accurate and reliable has been developed.

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Reference (9)

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Determination of the rosmarinic acid in Xiaokang capsule by HPLC

doi: 10.3969/j.issn.1006-0111.2015.06.020

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