Determination of oleanolic acid and ursolic acid in Shanzhajing Jiangzhi tablet by HPLC

LIN Yinan; JIN Huizi; SU Juan

doi:10.3969/j.issn.1006-0111.2015.05.018

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Objective To establish the quality standard for Shanzhajing Jiangzhi tablet by HPLC. Methods Samples were analyzed on a Agilent Zorbax SB C₁₈ column(250 mm×4.6 mm,5 μm) with methanol and 0.06 mol/L ammonium acetate(85:15)as mobile phase at the flow rate of 0.8 ml/min. The wavelength and column temperature were set at 210 nm and 25 ℃, respectively. Results The calibration curve showed a good linear relationship within the range of 0.124-2.48 μg for oleanolic acid (r=0.999 7) and 0.498-9.96 μg for ursolic acid (r=0.999 8). The average recovery ratio of the oleanolic acid and ursolic acid were 96.9% (RSD=1.26%) and 100.4% (RSD=2.6%), respectively. Conclusion The method was proved to be good at evaluation effectiveness and practicality.

Determination of oleanolic acid and ursolic acid in Shanzhajing Jiangzhi tablet by HPLC

1. School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China

2. Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received Date: 2014-07-09

Rev Recd Date: 2014-11-07

Key words:

Abstract: Objective To establish the quality standard for Shanzhajing Jiangzhi tablet by HPLC. Methods Samples were analyzed on a Agilent Zorbax SB C₁₈ column(250 mm×4.6 mm,5 μm) with methanol and 0.06 mol/L ammonium acetate(85:15)as mobile phase at the flow rate of 0.8 ml/min. The wavelength and column temperature were set at 210 nm and 25 ℃, respectively. Results The calibration curve showed a good linear relationship within the range of 0.124-2.48 μg for oleanolic acid (r=0.999 7) and 0.498-9.96 μg for ursolic acid (r=0.999 8). The average recovery ratio of the oleanolic acid and ursolic acid were 96.9% (RSD=1.26%) and 100.4% (RSD=2.6%), respectively. Conclusion The method was proved to be good at evaluation effectiveness and practicality.

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Reference (11)

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Determination of oleanolic acid and ursolic acid in Shanzhajing Jiangzhi tablet by HPLC

doi: 10.3969/j.issn.1006-0111.2015.05.018

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