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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2015  >  33(5): 438-440

QIAO Liye, FANG Li, CAO Yanli, LU Yin, SU Hua, WANG Shudong. Determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xinbaoshen tablets by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(5): 438-440. doi: 10.3969/j.issn.1006-0111.2015.05.015
Citation: QIAO Liye, FANG Li, CAO Yanli, LU Yin, SU Hua, WANG Shudong. Determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xinbaoshen tablets by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(5): 438-440. doi: 10.3969/j.issn.1006-0111.2015.05.015
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Determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xinbaoshen tablets by HPLC

doi: 10.3969/j.issn.1006-0111.2015.05.015

  • Department of Preparation, Nanjing General Hospital of Nanjing Military Region, PLA, Nanjing 210002, China

  • Received Date: 2014-05-27
  • Rev Recd Date: 2014-07-30
  • Objective To establish method of the determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xinbaoshen tablet,for the production of quality protection. Methods HPLC was performed on a Lichrospher-C18 column(250 mm×4.6mm,5 μm)with the mobile phase of methanol-0.1% H3PO4 (70:30).The flow rate was 1.0 ml/min,the column temperature was at 35 ℃ and the detection wavelength was 254 nm. Results The linear rang of aloe-emodin,rhein,emodin,chrysophanol and physcion were obtained between 2.30-18.4 μg/ml ( r=1.0),2.930-23.44 μg/ml( r=1.0),5.00-40.0 μg/ml ( r=1.0),14.870-118.96 μg/ml( r= 0.9998 ) and 7.410-59.28 μg/ml(r=0.999 9),the average recoveries were 100.16%,102.91%,99.76%,100.32%,100.44%,RSD were 1.58%,1.27%,1.67%,1.33%,1.03% (n=9). Conclusion The improved method was accurate and feasible which could be used convenient in quality control.
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    [2] 李 平,郑艳春,杨冬丽,等.HPLC同时测定新健胃包芯片中5种蒽醌类成分的含量[J].中国现代中药,2013,15(5):406-409.
    [3] 徐守竹,谢艳华,张晓卫,等.RP-HPLC同时测定肾康注射液中5种蒽醌类成分[J].中成药,2010,26(21):90-93.
    [4] 曾 芳,李 媛.大黄有效化学成分及其药理作用[J].当代医学,2013,19(12):149-150.
    [5] 王昕怡,邵加庆.肾功能不全患者的降糖治疗[J].医学研究生学报,2013,26(4):442-446.
    [6] 林小凤,李明权,李 琦,等.慢性肾脏病的中西医治疗进展[J].黑龙江医药,2012,1:43-45.
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Determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xinbaoshen tablets by HPLC

doi: 10.3969/j.issn.1006-0111.2015.05.015
  • Department of Preparation, Nanjing General Hospital of Nanjing Military Region, PLA, Nanjing 210002, China
  • Received Date: 2014-05-27
  • Rev Recd Date: 2014-07-30

Abstract: Objective To establish method of the determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xinbaoshen tablet,for the production of quality protection. Methods HPLC was performed on a Lichrospher-C18 column(250 mm×4.6mm,5 μm)with the mobile phase of methanol-0.1% H3PO4 (70:30).The flow rate was 1.0 ml/min,the column temperature was at 35 ℃ and the detection wavelength was 254 nm. Results The linear rang of aloe-emodin,rhein,emodin,chrysophanol and physcion were obtained between 2.30-18.4 μg/ml ( r=1.0),2.930-23.44 μg/ml( r=1.0),5.00-40.0 μg/ml ( r=1.0),14.870-118.96 μg/ml( r= 0.9998 ) and 7.410-59.28 μg/ml(r=0.999 9),the average recoveries were 100.16%,102.91%,99.76%,100.32%,100.44%,RSD were 1.58%,1.27%,1.67%,1.33%,1.03% (n=9). Conclusion The improved method was accurate and feasible which could be used convenient in quality control.

QIAO Liye, FANG Li, CAO Yanli, LU Yin, SU Hua, WANG Shudong. Determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xinbaoshen tablets by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(5): 438-440. doi: 10.3969/j.issn.1006-0111.2015.05.015
Citation: QIAO Liye, FANG Li, CAO Yanli, LU Yin, SU Hua, WANG Shudong. Determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Xinbaoshen tablets by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(5): 438-440. doi: 10.3969/j.issn.1006-0111.2015.05.015
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