ZHOU Yangyun, FENG Xiaolu, WANG Yuliang, ZHANG Lei. Analysis of chemical constituents in Capparis spinosa by GC-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(5): 436-437,480. doi: 10.3969/j.issn.1006-0111.2015.05.014
| Citation:
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ZHOU Yangyun, FENG Xiaolu, WANG Yuliang, ZHANG Lei. Analysis of chemical constituents in Capparis spinosa by GC-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(5): 436-437,480. doi: 10.3969/j.issn.1006-0111.2015.05.014
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Analysis of chemical constituents in Capparis spinosa by GC-MS
- 1.
School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
- 2.
School of Agricultural and Biology, Shanghai Jiaotong University, Shanghai 201101, China
- 3.
School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China;Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- Received Date: 2015-01-28
- Rev Recd Date:
2015-03-30
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Abstract
Objective To analyze the chemical constituents in fruits of Capparis spinosa by GC-MS analysis. Methods The compounds were extracted from Capparis spinosa by 70% ethanol after smash. After extraction by petroleum ether (boiling range was from 60 to 90 ℃), the compounds were analyzed by gas chromatography with a VF-5ms capillary column. The column was heated up from 80 ℃ to 300 ℃, 15 ℃/min, then maintained for 10 minutes,vaporization temperature was 250 ℃, carrier gas was Helium and the flow rate was 1 ml/min. The detection was carried out by mass spectrometry using electron impact ion source with 70 eV ionization voltages. The ion source temperature was 200 ℃ and split ratio was 30:1. The injection volume was 1.0 μl. The content of the compounds was determined with peak area normalization method. Results 53 chromatographically peaks were separated and 48 chemical constituents were identified including hexadecanoic acid (21.82%), octadecanoic acid (7.49%), oleinic acid (42.93%) and monoolein (2.39%). These chemical constituents were mainly saturated fatty acid ester, unsaturated fatty acid ester and alkanes. Conclusion The method is rapid, accurate and sensitive with the usage of GC-MS, which could be used to identify main chemical constituents of Capparis spinosa in fruit.
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References
|
[1]
|
吾斯曼·吐尔逊.刺山柑(老鼠瓜)的药用探索[J].中国民族医药杂志,2006,4:33. |
|
[2]
|
Afsharypuor S, Jeiran K,Jazy AA.First investigation of the flavour profiles of the leaf,ripe fruit and root of Capparis spinosa var. mucronifolia from Iran[J].Pharm Acta Helv,1998,72:307-309. |
|
[3]
|
谢丽琼,马东建.维药刺山柑果实挥发油和脂肪酸成分的GC-MS研究[J].食品科学,2007,28(5):262-264. |
|
[4]
|
冯小路,路金才,辛海量,等.野西瓜果实超临界CO2流体萃取物的化学成分及药理活性[J].上海交通大学学报:农业科学版,2009,27(1):57-60. |
|
[5]
|
Menendez JA,Papadimitropoulou A,Vellon L,et al.A genomic explanation connecting "Mediterranean diet",olive oil and cancer:oleic acid,the main monounsaturated Fatty acid of olive oil,induces formation of inhibitory "PEA3 transcription factor-PEA3 DNA binding site" complexes at the Her-2/neu (erbB-2) oncogene promoter in breast,ovarian and stomach cancer cells[J].Eur J Cancer,2006,42(15):2425-2432. |
|
[6]
|
吴小娟,李红冰,逄 越,等.山茶和油茶种子中脂肪酸的分析[J].大连大学学报,2006,27(4):56-58. |
|
[7]
|
褚遵华,巩怀证,李国荣,等.维生素E的研究进展[J].职业与健康,2005,21(12):1901-1902. |
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