Combination of wogonin with 5-fluorouracil exhibits antagonistic effect on human Hep-G2 hepatocellular carcinoma cells

CHEN Weizhong; CAI Jingbin; CHEN Xiaolan; CHEN Ruiqi; LI Xiajing; LIAO Lianming

doi:10.3969/j.issn.1006-0111.2015.05.007

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Objective To investigate the antagonistic effect of wogonin in combination with 5-fluorouracil (5-FU) on the proliferation of human Hep-G2 hepatocellular carcinoma cells. Methods Human hepatocellular Hep-G2 cells were divided into experimental group (wogonin group, 5-FU group, wogonin + 5-FU group) and control group. MTT method was used to evaluate tumor cell proliferation in vitro, flow cytometry analysis was used to evaluate tumor cell apoptosis. Results The results showed that the wogonin inhibited the proliferation of tumor cells at the concentrations of 5, 10, 20 and 40 μmol/L after 24 h and 48 h treatment respectively (P<0.05); 5-FU also inhibited the proliferation of tumor cells at the concentrations of 5, 10, 20 and 40 mg/L after 24 h and 48 h treatment respectively (P<0.05). However when wogonin was combined with 5-FU (wogonin+5-FU group), an antagonistic effect was observed on tumor cell proliferation (P<0.05). When cells were treated by wogonin+5-FU for 48 h, the combined index (CI) value slowed a dose-dependent antagonistic effect (P<0.05). Conclusion Wogonin has anti-tumor effect. However when wogonin was combined with 5-FU, an obvious antagonistic effect on 5-FU's anti-tumor action was observed. The underlying mechanism deserves further study.

Combination of wogonin with 5-fluorouracil exhibits antagonistic effect on human Hep-G2 hepatocellular carcinoma cells

1. First Affiliated Hospital, Fuzhou General Hospital of Nanjing Military Region, Putian 351100, China

2. Department of Oncology, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China

Received Date: 2014-11-20

Rev Recd Date: 2015-07-09

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Abstract: Objective To investigate the antagonistic effect of wogonin in combination with 5-fluorouracil (5-FU) on the proliferation of human Hep-G2 hepatocellular carcinoma cells. Methods Human hepatocellular Hep-G2 cells were divided into experimental group (wogonin group, 5-FU group, wogonin + 5-FU group) and control group. MTT method was used to evaluate tumor cell proliferation in vitro, flow cytometry analysis was used to evaluate tumor cell apoptosis. Results The results showed that the wogonin inhibited the proliferation of tumor cells at the concentrations of 5, 10, 20 and 40 μmol/L after 24 h and 48 h treatment respectively (P<0.05); 5-FU also inhibited the proliferation of tumor cells at the concentrations of 5, 10, 20 and 40 mg/L after 24 h and 48 h treatment respectively (P<0.05). However when wogonin was combined with 5-FU (wogonin+5-FU group), an antagonistic effect was observed on tumor cell proliferation (P<0.05). When cells were treated by wogonin+5-FU for 48 h, the combined index (CI) value slowed a dose-dependent antagonistic effect (P<0.05). Conclusion Wogonin has anti-tumor effect. However when wogonin was combined with 5-FU, an obvious antagonistic effect on 5-FU's anti-tumor action was observed. The underlying mechanism deserves further study.

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Reference (10)

[1]	Zhao L, Chen Z, Zhao Q, et al. Developmental toxicity and genotoxicity studies of wogonin[J]. Regul Toxicol Pharmacol, 2011, 60(2): 212-217.
[2]	Liu Y, Wang X, Wang Y, et al. Combination of SNX-2112 with 5-FU exhibits antagonistic effect in esophageal cancer cells[J]. Int J Oncol, 2015,46(1):299-307.
[3]	Chou TC, Talalay P. Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs or enzyme inhibitors[J]. Adv Enzyme Regul, 1984, 22: 27-55.
[4]	Zhao Q, Wang J, Zou MJ, et al. Wogonin potentiates the antitumor effects of low dose 5-fluorouracil against gastric cancer through induction of apoptosis by down-regulation of NF-kappaB and regulation of its metabolism[J]. Toxicol Lett,2010, 197(3): 201-210.
[5]	Li CJ, Chu CY, Huang LH, et al. Synergistic anticancer activity of triptolide combined with cisplatin enhances apoptosis in gastric cancer in vitro and in vivo[J]. Cancer Lett, 2012, 319(2): 203-213.
[6]	Lee YM, Cheng PY, Chen SY, et al. Wogonin suppresses arrhythmias, inflammatory responses, and apoptosis induced by myocardial ischemia/reperfusion in rats[J]. J Cardiovasc Pharmacol, 2011, 58(2): 133-142.
[7]	Tsai CF, Yeh WL, Huang SM, et al. Wogonin induces reactive oxygen species production and cell apoptosis in human glioma cancer cells[J]. Int J Mol Sci, 2012, 13(8): 9877-9892.
[8]	Huang KF, Zhang GD, Huang YQ, et al. Wogonin induces apoptosis and down-regulates survivin in human breast cancer MCF-7 cells by modulating PI3K-AKT pathway[J]. Int Immunopharmacol, 2012, 12(2): 334-341.
[9]	Zhou M, Song X, Huang Y, et al. Wogonin inhibits H2O2-induced angiogenesis via suppressing PI3K/Akt/NF-kappaB signaling pathway[J]. Vasc Pharmacol, 2014, 60(3): 110-119.
[10]	Zhao K, Song X, Huang Y, et al. Wogonin inhibits LPS-induced tumor angiogenesis via suppressing PI3K/Akt/NF-kappaB signaling[J]. Eur J Pharmacol, 2014, 737: 57-69.

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Combination of wogonin with 5-fluorouracil exhibits antagonistic effect on human Hep-G2 hepatocellular carcinoma cells

doi: 10.3969/j.issn.1006-0111.2015.05.007

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