LV Fang, ZHOU Jin, FEI Yang, CHEN Yu, ZHANG Guoqing, ZHAO Liang. Determination of asiatic acid in the root of Actinidia valvata by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 356-358. doi: 10.3969/j.issn.1006-0111.2015.04.017
| Citation:
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LV Fang, ZHOU Jin, FEI Yang, CHEN Yu, ZHANG Guoqing, ZHAO Liang. Determination of asiatic acid in the root of Actinidia valvata by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 356-358. doi: 10.3969/j.issn.1006-0111.2015.04.017
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Determination of asiatic acid in the root of Actinidia valvata by HPLC
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Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital Affiliated to Second Military Medical University, Shanghai 200438, China
- Received Date: 2014-03-06
- Rev Recd Date:
2014-09-09
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Abstract
Objective To establish a method for the determination of asiatic acid in the root of Actinidia valvata. Methods HPLC-VWD was used in the analysis. The column was Agilent HC-C18 (4.6 mm×250 mm,5μm);the mobile phase was acetonitrile: (30 mmol/L) acetic acid amine solution (35:65); the flow rate was 1.0 ml/min; the temperature of column was 25 ℃; the detection wavelength was set at 210 nm; the injection volume was 25 μl; the running time was 35 min. Results Asiatic acid was separated with interference in baseline. The linear range was 25.30-506.0 μg/ml with linear correlation of 0.999 6 for asiatic acid. The result of intra-day and inter-day precisions were both within 5% (n=3), and the average recovery was 99.4% with RSD 1.9% (n=6). Conclusion The method was simple, rapid, accurate and convenient for quality control of asiatic acid in the root of Actinidia valvata.
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