The effects of sevofluraneon learning and memory of neonatal mice

SUN Jihui; LU Zhijun; Xin Yichun; Yu Buwei

doi:10.3969/j.issn.1006-0111.2015.04.014

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Objective To evaluate the effects of sevoflurane on learning and memory of neonatal mice. Methods 122 neonatalmice (7 days postnatal) were included in this study.72 of them were exposed to sevoflurane (1.0 or 0.5MAC,minimum alveolar concentration) or 40% O₂ for 2 h(hours). Morris water maze was performed 4 and 12 weeks after anesthesia. Latency and swimming speed during training, time on island and times across island during the research were recorded. The rest 50 mice was used in artery blood analysis during sevoflurane (1.0 or 0.5 MAC, 0,1,2 h). Results pH,PaO₂, PaCO₂, SaO₂ were stable during anesthesia. Latency in control group were significantly shorter than the two anesthesia groups 4 weeks after anesthesia and 3 days after the training. During the last 2 training days, the latency in 1.0 MAC sevoflurane-exposed mice were significantly longer than that of the 0.5 MAC group.12 weeks after anesthesia, the latency was still significantly longer in 1.0 MAC sevoflurane-exposed mice on the last training day. The time on island and/or times across island were significantly decreased in anesthesia groupsduring theresearch performed 4 weeks and 12 weeks after anesthesia. Conclusion Early exposure to sevoflurane leads to a concentration and time-depended persistent learning and memory deficits to neonatal mice.

The effects of sevofluraneon learning and memory of neonatal mice

1. School of Medicine, Tongji University, Shanghai 200092, China

2. Department of Anesthesia, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China

3. Department of Anesthesia, Luwan Branch of Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200020, China

Received Date: 2014-06-04

Rev Recd Date: 2014-10-27

Key words:

Abstract: Objective To evaluate the effects of sevoflurane on learning and memory of neonatal mice. Methods 122 neonatalmice (7 days postnatal) were included in this study.72 of them were exposed to sevoflurane (1.0 or 0.5MAC,minimum alveolar concentration) or 40% O₂ for 2 h(hours). Morris water maze was performed 4 and 12 weeks after anesthesia. Latency and swimming speed during training, time on island and times across island during the research were recorded. The rest 50 mice was used in artery blood analysis during sevoflurane (1.0 or 0.5 MAC, 0,1,2 h). Results pH,PaO₂, PaCO₂, SaO₂ were stable during anesthesia. Latency in control group were significantly shorter than the two anesthesia groups 4 weeks after anesthesia and 3 days after the training. During the last 2 training days, the latency in 1.0 MAC sevoflurane-exposed mice were significantly longer than that of the 0.5 MAC group.12 weeks after anesthesia, the latency was still significantly longer in 1.0 MAC sevoflurane-exposed mice on the last training day. The time on island and/or times across island were significantly decreased in anesthesia groupsduring theresearch performed 4 weeks and 12 weeks after anesthesia. Conclusion Early exposure to sevoflurane leads to a concentration and time-depended persistent learning and memory deficits to neonatal mice.

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Reference (19)

[1]	Kalkman CJ, Peelen L, Moons KG, et al. Behavior and development in children and age at the time of first anesthetic exposure[J]. Anesthesiology, 2009, 110(4): 805-812.
[2]	Wilder RT, Flick RP, Sprung J, et al. Early exposure to anesthesia and learning disabilities in a population-based birth cohort[J]. Anesthesiology, 2009, 110(4): 796-804.
[3]	Wise-Faberowski L, Zhang H, Ing R, et al.Isoflurane-induced neuronal degeneration: an evaluation in organotypic hippocampal slice culture [J].Anesth Analg, 2005, 101(3): 651-657.
[4]	Edwards DA, Shah HP, Cao W, et al. Bumetanide alleviates epileptogenic and neurotoxic effects of sevoflurane in neonatal rat brain[J]. Anesthesiology,2010,112(3): 567-575.
[5]	Kodama M, Satoh Y, Otsubo Y, et al. Neonatal desfluraneexposureinduces more robust neuroapoptosis than do isofluraneandsevoflurane and impairs working memory[J]. Anesthesiology,2011,115(5): 979-991.
[6]	Istaphanous GK, Howard J, Nan X, et al. Comparison of the neuroapoptoticproperties of equipotent anesthetic concentrationsofdesflurane, isoflurane, or sevoflurane in neonatal mice [J].Anesthesiology,2011, 114(3): 578-587.
[7]	刘万里,薛西,曹明芹,等.用SPSS实现完全随机设计多组比较秩和检验的多重比较[J].地方病通报, 2007,22(2): 27-29.
[8]	Bercker S, Bert B, BittigauP,etal.Neurodegeneration in newborn rats following propofolandsevoflurane anesthesia[J]. Neurotox Res,2009,16(2): 140-147.
[9]	Jevtovic-Todorovic V, Hartman RE, Izumi Y, et al. Earlyexposure to common anesthetic agents causes widespreadneurodegeneration in the developing rat brain and persistentlearning deficits[J]. J Neurosci,2003,23(3):876-882.
[10]	Liang G,Ward C, Peng J,et al. Isofluranecauses greater neurodegeneration than an equivalent exposureofsevoflurane in the developing brain of neonatal mice[J].Anesthesiology,2010,112(6): 1325-1334.
[11]	Loepke AW, McCann JC, Kurth CD, et al. The physiologiceffects of isoflurane anesthesia in neonatal mice[J].Anesth Analg, 2006, 102(1): 75-80.
[12]	Brambrink AM, Evers AS, Avidan MS, et al.Isoflurane-inducedneuroapoptosis in the neonatal rhesus macaquebrain[J]. Anesthesiology,2010, 112(4): 834-841.
[13]	Stratmann G, May LD, Sall JW, et al. Effect of hypercarbiaandisoflurane on brain cell death and neurocognitive dysfunctionin 7-day old rats[J]. Anesthesiology, 2009, 110(4): 849-861.
[14]	Vorhee CV, Williams MT. Morris water maze: procedures for assessing spatial and related forms of learning and memory[J]. Nat Prot,2006, 1(2): 848-858.
[15]	Zhou X, Song FH, He W, et al. Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus[J]. Mol Med Rep, 2012, 6(3): 543-546.
[16]	Ramage TM, Chang FL, Shih J, et al. Distinct long-term neurocognitive outcomes after equipotent sevoflurane or isofluraneanaesthesia in immature rats[J]. Br J Anaesth, 2013, 110 (S1): i39-i46.
[17]	Shelton KL.Discriminative stimulus effects of inhaled 1,1,1-trichloroethane in mice: comparison to other hydrocarbon vapors and volatile anesthetics[J]. Psychopharmacology (Berl),2009,203(2): 431-440.
[18]	Nishikawa K, Harrison NL.The actions of sevoflurane and desflurane on the gamma-aminobutyric acid receptor type A: effects of TM2 mutations in the alpha and betasubunits[J]. Anesthesiology,2003,99(3): 678-684.
[19]	Daval JL, Vert P. Apoptosis and neurogenesis after transienthypoxia in the developing rat brain[J]. Semin Perinatol,2004,28(4):257-263.

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The effects of sevofluraneon learning and memory of neonatal mice

doi: 10.3969/j.issn.1006-0111.2015.04.014

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