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Volume 33 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2015  >  33(4): 338-340,369

WANG Jinfeng, ZHAO Nan, WEI Ying, YANG Cuiyan, WANG Fang, LIU Dan. Optimized extraction technology of flos Puerariae lobata isoflavone using orthogonal test[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 338-340,369. doi: 10.3969/j.issn.1006-0111.2015.04.013
Citation: WANG Jinfeng, ZHAO Nan, WEI Ying, YANG Cuiyan, WANG Fang, LIU Dan. Optimized extraction technology of flos Puerariae lobata isoflavone using orthogonal test[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 338-340,369. doi: 10.3969/j.issn.1006-0111.2015.04.013
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Optimized extraction technology of flos Puerariae lobata isoflavone using orthogonal test

doi: 10.3969/j.issn.1006-0111.2015.04.013

  • No.208 Hospital of PLA, Changchun 130062, China

  • Received Date: 2013-09-29
  • Rev Recd Date: 2014-04-15
  • Objective To optimize the extraction technology of flos Puerariae lobata isoflavone. Methods The flos Puerariae lobata isoflavone was distilled by ethanol circumfluence. Total flavonoids, tectoridin and tectorigenin extracted from Puerariae using the UV and HPLC spectromertry methods were taken as evaluation indexes. Extraction technology was optimized with L9(34) orthogonal test on the base of single observation of ethanol concentration, solvent dosage and distilling time. Results The best extraction technology of flos Puerariae lobata isoflavone was: to add 12 times the amount of 70% ethanol for 90 minutes for the first time, and 10 times the amount of 70% ethanol for 60 minutes for the second time. Conclusion The optimized extraction process of flos Puerariae lobata isoflavone is reasonable and feasible, and it can offer reference to actual production.
  • [1] 尹俊亭,仲 英,孙敬勇,等.葛花的研究进展[J].中草药,2005;36(12):1905-1906.
    [2] Park EK,Shin YW,Lee HU,et al.Passive cutaneous anaphylaxis-inhibitory action of tectorigenin,a metabolite of tectoridin by intestinal microflora[J].Biol Pharm Bull,2004,27(7): 1099-1102.
    [3] Lee HW, Choo MK, Bae EA,et al.Beta-glucuronidase inhibitor tectorigenin isolated from the flower of Pueraria thunbergiana protects carbon tetrachloride-induced liver injury[J].Liver Int,2003,23(4):221-226.
    [4] Lee HU,Bae EA, Kim DH. Hepatoprotective effect of tectoridin and tectorigenin on tert-butyl hyperoxide-induced liver injury[J].J Pharmacol Sci, 2005, 97: 541-544.
    [5] Moon HI, Jung JC, Lee HK. Aldose reductase inhibitory effect by tectorigenin derivatives from Viola hondoensis[J].Bioorg Med Chem, 2006,14(22): 7592-7594.
    [6] 王金凤, 杨翠燕,张艳萍,等.鸢尾苷对高脂血症大鼠血清脂质的影响[J].基础医学与临床,2010,30(9):925-929.
    [7] 赵 楠,刘 丹,王艳萍,等。HPLC法测定葛花中鸢尾苷和鸢尾苷元含量[J].药学实践杂志,2012,30(3):226-228.
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Optimized extraction technology of flos Puerariae lobata isoflavone using orthogonal test

doi: 10.3969/j.issn.1006-0111.2015.04.013
  • No.208 Hospital of PLA, Changchun 130062, China
  • Received Date: 2013-09-29
  • Rev Recd Date: 2014-04-15

Abstract: Objective To optimize the extraction technology of flos Puerariae lobata isoflavone. Methods The flos Puerariae lobata isoflavone was distilled by ethanol circumfluence. Total flavonoids, tectoridin and tectorigenin extracted from Puerariae using the UV and HPLC spectromertry methods were taken as evaluation indexes. Extraction technology was optimized with L9(34) orthogonal test on the base of single observation of ethanol concentration, solvent dosage and distilling time. Results The best extraction technology of flos Puerariae lobata isoflavone was: to add 12 times the amount of 70% ethanol for 90 minutes for the first time, and 10 times the amount of 70% ethanol for 60 minutes for the second time. Conclusion The optimized extraction process of flos Puerariae lobata isoflavone is reasonable and feasible, and it can offer reference to actual production.

WANG Jinfeng, ZHAO Nan, WEI Ying, YANG Cuiyan, WANG Fang, LIU Dan. Optimized extraction technology of flos Puerariae lobata isoflavone using orthogonal test[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 338-340,369. doi: 10.3969/j.issn.1006-0111.2015.04.013
Citation: WANG Jinfeng, ZHAO Nan, WEI Ying, YANG Cuiyan, WANG Fang, LIU Dan. Optimized extraction technology of flos Puerariae lobata isoflavone using orthogonal test[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 338-340,369. doi: 10.3969/j.issn.1006-0111.2015.04.013
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