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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2015  >  33(1): 58-59,82

WU Heqin, CHEN Zhaoxia. Determination of fluoxetine hydrochloride by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 58-59,82. doi: 10.3969/j.issn.1006-0111.2015.01.015
Citation: WU Heqin, CHEN Zhaoxia. Determination of fluoxetine hydrochloride by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 58-59,82. doi: 10.3969/j.issn.1006-0111.2015.01.015
shu

Determination of fluoxetine hydrochloride by HPLC

doi: 10.3969/j.issn.1006-0111.2015.01.015

  • Changzhou Siyao Pharmaceutical Limited Company, Changzhou 213004, China

  • Received Date: 2013-05-22
  • Rev Recd Date: 2014-04-07
  • Objective To establish an HPLC method for assay determination of fluoxetine hydrochloride. Methods Agilent Eclipse XDB-C8 (4.6 mm × 250 mm,5 μm) was used,mobile phase was tetrahydrofuran-methanol-triethylamine buffer (to 10 ml of triethylamine in a 1 000 ml flask,added 980 ml of water,pH was adjust to 6.0 by phosphoric acid) (30:10:60),flow rate was 1.0 ml/min,detection wavelength was 227 nm,injection volume was 10 μl,column temperature was 25℃. Results Linearity range was 55.17-165.51 μg/ml (r=0.999 9),minimum detection limit was 0.15 μg/ml,accuracy was between 99.9%-100.0%,repeatability RSD was 0.1% (n=6). Conclusion The method was accurate and reliable,which could be applied for quality control of fluoxetine hydrochloride.
  • [1] 周亦卫,张 圩,王建平,等.抗抑郁新药——盐酸氟西汀[J].上海化工杂志,1998,23(22):28-30.
    [2] 国家药品监督管理局标准(试行).盐酸氟西汀质量标准[S].[WS-551(X-434)-98].
    [3] 吕竹芬,谢清春,申 楼.HPLC测定盐酸氟西汀胶囊的含量[J].广东药学杂志,2005,15(3):13-15.
    [4] 熊凤梅,木合塔尔·吐尔洪,木尼热·阿布都克力木,等.用毛细管电泳电化学发光法测定盐酸氟西汀的含量[J].药物分析杂志,2008,28(3):440-442.
    [5] The British Pharmacopeial Convention.British Pharmacopeia (6th ed)[S].2011:938-939.
    [6] The European Pharmacopeial Convention.European Pharmacopeia[S].(7 th ed),2011:2048-2049.
    [7] The United States Pharmacopeial Convention.U.S.Pharmacopeia[S].(35 th ed),2012:3241-3242.
    [8] 国家药典委员会.中华人民共和国药典2010年版 二部[S].北京:中国医药科技出版社,2010:194-195.
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Determination of fluoxetine hydrochloride by HPLC

doi: 10.3969/j.issn.1006-0111.2015.01.015
  • Changzhou Siyao Pharmaceutical Limited Company, Changzhou 213004, China
  • Received Date: 2013-05-22
  • Rev Recd Date: 2014-04-07

Abstract: Objective To establish an HPLC method for assay determination of fluoxetine hydrochloride. Methods Agilent Eclipse XDB-C8 (4.6 mm × 250 mm,5 μm) was used,mobile phase was tetrahydrofuran-methanol-triethylamine buffer (to 10 ml of triethylamine in a 1 000 ml flask,added 980 ml of water,pH was adjust to 6.0 by phosphoric acid) (30:10:60),flow rate was 1.0 ml/min,detection wavelength was 227 nm,injection volume was 10 μl,column temperature was 25℃. Results Linearity range was 55.17-165.51 μg/ml (r=0.999 9),minimum detection limit was 0.15 μg/ml,accuracy was between 99.9%-100.0%,repeatability RSD was 0.1% (n=6). Conclusion The method was accurate and reliable,which could be applied for quality control of fluoxetine hydrochloride.

WU Heqin, CHEN Zhaoxia. Determination of fluoxetine hydrochloride by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 58-59,82. doi: 10.3969/j.issn.1006-0111.2015.01.015
Citation: WU Heqin, CHEN Zhaoxia. Determination of fluoxetine hydrochloride by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 58-59,82. doi: 10.3969/j.issn.1006-0111.2015.01.015
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