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Volume 33 Issue 1
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2015  >  33(1): 53-54,57

WANG Hui, LI Yang, LÜ Fang, LING Shuihua, HUANG Yufeng, ZHANG Guoqing. Determination of calycosin contents in Wujin capsule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 53-54,57. doi: 10.3969/j.issn.1006-0111.2015.01.013
Citation: WANG Hui, LI Yang, LÜ Fang, LING Shuihua, HUANG Yufeng, ZHANG Guoqing. Determination of calycosin contents in Wujin capsule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 53-54,57. doi: 10.3969/j.issn.1006-0111.2015.01.013
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Determination of calycosin contents in Wujin capsule by HPLC

doi: 10.3969/j.issn.1006-0111.2015.01.013

  • Department of Pharmacy, Eastern Hepatobilliary Hospital, Second Military Medical University, Shanghai 200438, China

  • Received Date: 2014-01-27
  • Rev Recd Date: 2014-04-11
  • Objective To explore method for determination of calycosin contents in Wujin capsule by HPLC. Methods The sample was studied on ZORBA Bonus-RP (4.6 mm×260 mm,5 μm) column,the mobile phase consisted of acetonitrile and H2O-0.1% HCOOH (gradient elution),the temperature of column was 25℃,the UV detection wavelength was set at 260 nm,flow speed was 1.0 ml/min and injection volume was 10 μl. Results The calycosin calibration curve was at the range of 7.4-117.6 μg/ml (r=1.000),with their regression function being Y=34.002 X-3.451 2.The recovery of calycosin was 95.24% (RSD=2.10%).6 batches of Wujin capsule calycosin contents were 9.26-23.14 μg/g. Conclusion HPLC was an accuracy and simple method for determining the content of calycosin in Wujin capsule.
  • [1] 国家药典委员会.中华人民共和国药典2010年版一部[S].北京:中国医药科技出版社,2010:283-284.
    [JP3]贺云彪,黄兰芳,胡 伟,等.高效液相色谱测定黄芪中毛蕊异黄酮和芒柄花素的含量[J].光谱实验室,2010,27(4):1541-1545.
    [3] 林 青,李 媛,谭晓梅,等.LC-MS/MS同时测定大鼠血浆中黄芪成分芒柄花素、毛蕊异黄酮和异鼠李素的浓度及其药动学研究[J].中药材,2013,36(4):589-593.
    [4] Chen J,Zhao XG,Ye Y,et al.Estrogen receptor beta-mediated proliferative inhibition and apoptosis in human breast cancer by calycosin and formononetin[J].Cell Physiol Biochem,2013,32(6):1790-1797.
    [5] Xu YH,Xiong JF,Wang SS,et al.Calycosin entered HUVECs and ameliorated AGEs-promoted cell apoptosis via the Bcl-2 pathway[J].J Nat Med,2014,68(1):163-172.
    [6] 谢娟平.D101大孔树脂纯化犁头草黄酮的研究[J].陕西农业科学,2010,56(5):61-63.
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Determination of calycosin contents in Wujin capsule by HPLC

doi: 10.3969/j.issn.1006-0111.2015.01.013
  • Department of Pharmacy, Eastern Hepatobilliary Hospital, Second Military Medical University, Shanghai 200438, China
  • Received Date: 2014-01-27
  • Rev Recd Date: 2014-04-11

Abstract: Objective To explore method for determination of calycosin contents in Wujin capsule by HPLC. Methods The sample was studied on ZORBA Bonus-RP (4.6 mm×260 mm,5 μm) column,the mobile phase consisted of acetonitrile and H2O-0.1% HCOOH (gradient elution),the temperature of column was 25℃,the UV detection wavelength was set at 260 nm,flow speed was 1.0 ml/min and injection volume was 10 μl. Results The calycosin calibration curve was at the range of 7.4-117.6 μg/ml (r=1.000),with their regression function being Y=34.002 X-3.451 2.The recovery of calycosin was 95.24% (RSD=2.10%).6 batches of Wujin capsule calycosin contents were 9.26-23.14 μg/g. Conclusion HPLC was an accuracy and simple method for determining the content of calycosin in Wujin capsule.

WANG Hui, LI Yang, LÜ Fang, LING Shuihua, HUANG Yufeng, ZHANG Guoqing. Determination of calycosin contents in Wujin capsule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 53-54,57. doi: 10.3969/j.issn.1006-0111.2015.01.013
Citation: WANG Hui, LI Yang, LÜ Fang, LING Shuihua, HUANG Yufeng, ZHANG Guoqing. Determination of calycosin contents in Wujin capsule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 53-54,57. doi: 10.3969/j.issn.1006-0111.2015.01.013
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