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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2015  >  33(1): 49-52,71

LÜ Yanan, TAN Guangguo. Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 49-52,71. doi: 10.3969/j.issn.1006-0111.2015.01.012
Citation: LÜ Yanan, TAN Guangguo. Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 49-52,71. doi: 10.3969/j.issn.1006-0111.2015.01.012
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Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS

doi: 10.3969/j.issn.1006-0111.2015.01.012

  • No.22 Hospital of PLA, Golmud 816000, China

  • Received Date: 2013-08-09
  • Rev Recd Date: 2014-01-11
  • Objective To analyze chemical constituents of Xionggui Decotion by rapid-resolution (high performance) liquid chromatography-time of flight mass spectrometry (HPLC-TOF-MS). Methods A Shelloseido column(100 mm×3.0 mm,3 μm)was used to separate.The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute.The flow rate was 0.4 ml/min.TOF-MS was applied for qualitative analysis under positive ion mode. Results Under LC/MS condition,47 major constituents in Xionggui Decotion were identified by time of flight mass spectrometry and structure-relevant fragment ions. Conclusion A simple and reliable method using HPLC-TOF-MS was established to identify the chemical constituents of Xionggui Decotion.
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Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS

doi: 10.3969/j.issn.1006-0111.2015.01.012
  • No.22 Hospital of PLA, Golmud 816000, China
  • Received Date: 2013-08-09
  • Rev Recd Date: 2014-01-11

Abstract: Objective To analyze chemical constituents of Xionggui Decotion by rapid-resolution (high performance) liquid chromatography-time of flight mass spectrometry (HPLC-TOF-MS). Methods A Shelloseido column(100 mm×3.0 mm,3 μm)was used to separate.The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute.The flow rate was 0.4 ml/min.TOF-MS was applied for qualitative analysis under positive ion mode. Results Under LC/MS condition,47 major constituents in Xionggui Decotion were identified by time of flight mass spectrometry and structure-relevant fragment ions. Conclusion A simple and reliable method using HPLC-TOF-MS was established to identify the chemical constituents of Xionggui Decotion.

LÜ Yanan, TAN Guangguo. Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 49-52,71. doi: 10.3969/j.issn.1006-0111.2015.01.012
Citation: LÜ Yanan, TAN Guangguo. Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 49-52,71. doi: 10.3969/j.issn.1006-0111.2015.01.012
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