DOU Jiatai, Li Shengwen. Determination of content of cucurbitacin B in cucurbitacins by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(4): 294-296. doi: 10.3969/j.issn.1006-0111.2014.04.015
| Citation:
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DOU Jiatai, Li Shengwen. Determination of content of cucurbitacin B in cucurbitacins by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(4): 294-296. doi: 10.3969/j.issn.1006-0111.2014.04.015
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Determination of content of cucurbitacin B in cucurbitacins by HPLC
- Received Date: 2012-11-13
- Rev Recd Date:
2013-04-08
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Abstract
Objective To determina the content of cucurbitacin B in cucurbitacins. Methods The highly effective liquid phase chromatography was used,with Waters Symmetry C18 (250 mm× 4.6 mm 5 μm) column.The mobile phase was acetonitrile-water (51:49), the flow rate was 1 ml/min, column temperature was 25℃, the detection wavelength was 228 nm. Results Cucurbitacin B regression equation: Y=1 067.3C-0.508 4 (r=0.999 9), the average recovery rate was 99.39%(n=6), RSD was 0.56%, which showed that this method had a good recovery rate. Conclusion The HPLC method for the determination of content of cucurbitacin B in cucurbitacins was simple, reproducible, accurate.
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