Detection of glinides adulterated in traditional Chinese medicine for diabetes by TLC-SERS

QIN Jianhong

doi:10.3969/j.issn.1006-0111.2014.03.011

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Objective To establish detection method of glinides adulterated illegally in hypoglycemic traditional Chinese medicine (TCM) by surface enhanced Raman spectroscopy (SERS). Methods Adulterated chemicals and TCM matrixes were separated by TLC first. Then trace substances in TLC plate was tested by SERS method. By investigating SERS detection conditions of glinides chemicals in simulated positive samples, a detection method was established to detect illegal adulterant in hypoglycemic TCM. Results Better SERS spectra of glinides could be obtained by silver sol prepared with organic solvent DMF. Conclusion The detection method coupled with TLC and SERS in this paper was simple, fast and economical which could be used to detect glinides adulterated illegally in hypoglycemic Chinese patent medicine quickly.

Detection of glinides adulterated in traditional Chinese medicine for diabetes by TLC-SERS

Department of Pharmacy, No. 169 Hospital of PLA, Hengyang 421001, China

Received Date: 2014-04-02

Rev Recd Date: 2014-04-21

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Abstract: Objective To establish detection method of glinides adulterated illegally in hypoglycemic traditional Chinese medicine (TCM) by surface enhanced Raman spectroscopy (SERS). Methods Adulterated chemicals and TCM matrixes were separated by TLC first. Then trace substances in TLC plate was tested by SERS method. By investigating SERS detection conditions of glinides chemicals in simulated positive samples, a detection method was established to detect illegal adulterant in hypoglycemic TCM. Results Better SERS spectra of glinides could be obtained by silver sol prepared with organic solvent DMF. Conclusion The detection method coupled with TLC and SERS in this paper was simple, fast and economical which could be used to detect glinides adulterated illegally in hypoglycemic Chinese patent medicine quickly.

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Detection of glinides adulterated in traditional Chinese medicine for diabetes by TLC-SERS

doi: 10.3969/j.issn.1006-0111.2014.03.011

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