YANG Wenhui, XIAO Qiusheng, JIAG Zongwen, ZHANG Su, SONG Haiyan, Wang Dianrong. In vitro release of thermosensitive slow-release rectal liquid suppository of insulin[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 56-58,67. doi: 10.3969/j.issn.1006-0111.2014.01.016
| Citation:
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YANG Wenhui, XIAO Qiusheng, JIAG Zongwen, ZHANG Su, SONG Haiyan, Wang Dianrong. In vitro release of thermosensitive slow-release rectal liquid suppository of insulin[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 56-58,67. doi: 10.3969/j.issn.1006-0111.2014.01.016
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In vitro release of thermosensitive slow-release rectal liquid suppository of insulin
- 1.
School of Pharmacy, Yanbian University, Yanji 133000, China;No. 208th Hospital of PLA, Changchun 130021, China
- 2.
No. 208th Hospital of PLA, Changchun 130021, China
- 3.
School of Pharmacy, Yanbian University, Yanji 133000, China
- 4.
The Vet Ranch of Haotetaohai, Inner Mongolia Hailaer 021000, China
- Received Date: 2013-06-05
- Rev Recd Date:
2013-11-21
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Abstract
Objective To establish HPLC method for the content determination of insulin in thermosensitive slow-release rectal liquid suppository, evaluate release characteristics in vitro. Methods The separation was performed on a Welch ultimate column(4.6 mm×250 mm, 5 μm).The mobile phase was composed of 0.2 mol/L sodium sulfate buffer (pH was adjusted to 2.3 with ethanolamine)-acetonitrile (74:26) with flow rate 1ml/min, detected at 214 nm, the insulin levels were determined. Adopting membrane less release model, bovine intestinal mucosa release model, the gut mucosa release model, insulin was used as model drug, drug release mechanism of thermosensitive slow-release rectal liquid suppository of insulin in vitro was evaluate. Results The insulin in situ gel was linear in the range of 1.0-40.0 U/ml(r=0.999 1).The average recovery was 99.35%, RSD was 0.79;membrane-less release:Q=0.495 4t-2.611 8(r=0.997 7);bovine intestinal mucosa release:(1-Q)1/3=0.001 6t+2.927 4(r=0.975 5);gut-mucosa release:lnQ=0.878 3lnt-4.931 1(r=0.970 5). Conclusion The method was simple, fine repeatability, sensitive, accurate and reliable. The method could be applicable to the preparation of the content determination of insulin and evaluate release mechanism thermosensitive slow-release rectal liquid suppository of insulin.
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