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Volume 31 Issue 6
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2013  >  31(6): 459-461

WANG Hui, LU Ke-jun, ZHANG Jie-jin, LUAN Xiao-lin, ZHOU Yan-ni, ZHANG Guo-qing. Determination of silybin contents in Gan'an capsule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(6): 459-461. doi: 10.3969/j.issn.1006-0111.2013.06.017
Citation: WANG Hui, LU Ke-jun, ZHANG Jie-jin, LUAN Xiao-lin, ZHOU Yan-ni, ZHANG Guo-qing. Determination of silybin contents in Gan'an capsule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(6): 459-461. doi: 10.3969/j.issn.1006-0111.2013.06.017
shu

Determination of silybin contents in Gan'an capsule by HPLC

doi: 10.3969/j.issn.1006-0111.2013.06.017

  • Departement of Pharmacy, Eastern Hepatobilliary Hospital, Second Military Medical University, Shanghai 200438, China

  • Received Date: 2012-09-26
  • Rev Recd Date: 2013-03-26
  • Objective To establish a HPLC method for determination of silybin contents in Gan'an capsule. Methods Angilent Zorbax SB C18(4.6 mm×250 mm, 5 μm) column was used with 1% acetic acid:methanol=50:50 as the mobile phase. The flow rate was 1.0 ml/min, and the temperature was at 30℃.The detection wavelength was 287 nm.Determination of silybin contents in Gan'an capsule of 10 batches of preparations. Results The silybin calibration curve was at the range of 10.28~123.36 μg/ml(r=1)with average recovery of 97.00%(RSD=0.68%).10 batches of preparations silybin contents in Gan'an capsule was 11.11~16.65 mg/granule. Conclusion The method was simple accurate and reproducible which could be used for the quality control of Gan'an capsule.
  • [1] 任冰如,赵友谊,徐柏衡,等.水飞蓟素提取和水飞蓟宾纯化的研究[J].时珍国医国药,2012,23(3):655.
    [2] 梁 敏,宋粉云,吕朝霞.HPLC法测定益肝灵片中水飞蓟宾的含量[J].广东药学院学报,2008,24 (5):472.
    [3] 周采菊,黄华瑞. HPLC法测定水飞蓟素及制剂中水飞蓟宾的含量[J].中国药品标准,2008,9(3):235.
    [4] Xin RL,Yan FL,Yan XZ,et al. Determination of silymarin in microemulsion by RP-HPLC[J]. J Chin Pharm Sci,2009,18(2):190.
    [5] 国家药典委员会.中华人民共和国药典2010年版一部[S].北京:中国医药科技出版社, 2010:76.
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Determination of silybin contents in Gan'an capsule by HPLC

doi: 10.3969/j.issn.1006-0111.2013.06.017
  • Departement of Pharmacy, Eastern Hepatobilliary Hospital, Second Military Medical University, Shanghai 200438, China
  • Received Date: 2012-09-26
  • Rev Recd Date: 2013-03-26

Abstract: Objective To establish a HPLC method for determination of silybin contents in Gan'an capsule. Methods Angilent Zorbax SB C18(4.6 mm×250 mm, 5 μm) column was used with 1% acetic acid:methanol=50:50 as the mobile phase. The flow rate was 1.0 ml/min, and the temperature was at 30℃.The detection wavelength was 287 nm.Determination of silybin contents in Gan'an capsule of 10 batches of preparations. Results The silybin calibration curve was at the range of 10.28~123.36 μg/ml(r=1)with average recovery of 97.00%(RSD=0.68%).10 batches of preparations silybin contents in Gan'an capsule was 11.11~16.65 mg/granule. Conclusion The method was simple accurate and reproducible which could be used for the quality control of Gan'an capsule.

WANG Hui, LU Ke-jun, ZHANG Jie-jin, LUAN Xiao-lin, ZHOU Yan-ni, ZHANG Guo-qing. Determination of silybin contents in Gan'an capsule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(6): 459-461. doi: 10.3969/j.issn.1006-0111.2013.06.017
Citation: WANG Hui, LU Ke-jun, ZHANG Jie-jin, LUAN Xiao-lin, ZHOU Yan-ni, ZHANG Guo-qing. Determination of silybin contents in Gan'an capsule by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(6): 459-461. doi: 10.3969/j.issn.1006-0111.2013.06.017
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