Analysis of toxic ingredients of Xiao Chai Hu Tang

ZHAO Bai-yun; LI Yue-yue; ZHU Zhen-yu; ZHAO Liang; GE Meng; ZHANG Guo-qing; CHAI Yi-feng

doi:10.3969/j.issn.1006-0111.2013.06.016

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Objective To determine whether Xiao Chai Hu Tang have liver toxicity and the sources of toxicity. Methods HPLC-TOF/MS method and the MTT were used to assay the Xiao Chai Hu Tang chemical composition and the survival of SMMC-7721 cells, respectively. Then the stepwise multiple linear regression was used to analysis the chemical composition of Xiao Chai Hu Tang which affected SMMC-7721 cells survival. Results The possible toxic component were found from Scutellaria:X3, chrysin-8-C-β-D-glucose the pyrano base-6-C-α in-L-Arab pyran-glucosidase or its isomers. Conclusion The method initially found potentially toxic constituents of Xiao Chai Hu Tang, which provided a research base for Attenuated compatibility, in order to optimize the prescription, develop the preparation process of science, and improve the quality of the preparations.

Analysis of toxic ingredients of Xiao Chai Hu Tang

1. Department of Pharmacy, Hangzhou Normal University Affiliated Hospital, Hangzhou 310000, China

2. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China

3. School of Pharmacy, Second Millitary Medical University, Shanghai 200433, China

Received Date: 2012-09-08

Rev Recd Date: 2012-12-08

Key words:

Abstract: Objective To determine whether Xiao Chai Hu Tang have liver toxicity and the sources of toxicity. Methods HPLC-TOF/MS method and the MTT were used to assay the Xiao Chai Hu Tang chemical composition and the survival of SMMC-7721 cells, respectively. Then the stepwise multiple linear regression was used to analysis the chemical composition of Xiao Chai Hu Tang which affected SMMC-7721 cells survival. Results The possible toxic component were found from Scutellaria:X3, chrysin-8-C-β-D-glucose the pyrano base-6-C-α in-L-Arab pyran-glucosidase or its isomers. Conclusion The method initially found potentially toxic constituents of Xiao Chai Hu Tang, which provided a research base for Attenuated compatibility, in order to optimize the prescription, develop the preparation process of science, and improve the quality of the preparations.

HTML

Reference (15)

[1]	陆婷婷,赵国平.小柴胡汤防治肝病机制研究进展[J].世界华人消化杂志,2008,16(9):971.
[2]	尹丽荣,陈泽雄.小柴胡汤治疗肝癌研究进展[J].中成药,2008,30(4):583.
[3]	杜矗森,黄秀深.小柴胡汤及组分抗肿瘤作用研究与分析[J].河北中医,2009,31(6):948.
[4]	金航.日本汉方药制剂副作用分析[J].国外医学中医中药分册,1997,19(3):62.
[5]	陈静. 浅论中药药物性肝病[J].湖北中医杂志,1999,21(5):202.
[6]	Melchart D,Linde K,Weidenhammer W,et al. Liver enzyme elevation in patients treated with traditional Chinese medicine[J]. JAMA,1999,282:28.
[7]	Chitturi S,Farrell GC. Herbal hepatotoxicity:An expanding but poorly defind problem[J]. J Gastroenterol Hepatol, 2000,15:1093.
[8]	Thomas D. Schiano, MD. Liver injury from herbs and other botanicals[J].Clin Liv Dis,1998,2(3):607.
[9]	彭龙玲,杨亚斯,曹毓,等.小柴胡汤提取物灌胃给药的长期毒性实验研究[J].时珍国医国药,2003,14(9):517.
[10]	孙振球.医学统计学[M].北京:人民卫生出版社,2010:245.
[11]	刘晓帆,娄子洋,朱臻宇,等.采用HPLC-TOF/MS对中药复方小柴胡汤中化学成分的快速分析鉴别[J].第二军医大学学报,2009, 8:941.
[12]	戈萌,柴逸峰,季红光,等.不同配伍组方小柴胡汤的人肝细胞毒性体外检测[J]. 第二军医大学学报,2007,11:1266.
[13]	赵翔,陈希,李常虹,等.白杨素对人肝癌BEL-7402细胞表面超微结构和蛋白质磷酸化及相关通路的影响[J]. 中国组织工程研究与临床康复,2011, 15(6):1050.
[14]	姜金生,韦英杰,贾晓斌,等.白杨素及其衍生物的药理作用和构效关系研究进展[J].中草药2011,42(11):2345.
[15]	韦敏,刘华钢,刘丽敏,等.白花丹素的体外肝毒性研究[J].时珍国医国药2011,42(11):1312.

Message Board

Analysis of toxic ingredients of Xiao Chai Hu Tang

doi: 10.3969/j.issn.1006-0111.2013.06.016

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Related

Proportional views