Experimental study on anti-oxidative effect of proanthocyanidins phospholipid complex in grape seed

MIAO Zhi-ru; YUAN Xia; LIAO Li-na; HOU Zhong-hai; JIANG Guo-jun

doi:10.3969/j.issn.1006-0111.2013.05.012

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Objective To study the antioxidant effects of grape seed proanthocyanidins phospholipid complex(GSPPC). Methods Eighty rats were randomly divided into eight groups: normal control, model control, Proanthocyanidins, Soybean phospholipids, Vitamin C and low dose of GSPPC, moderate dose of GSPPC, and high dose of GSPPC. Drugs were given orally to rats once a day for 30 days. And then, 50% ethanol was given to rats once by oral gavages to make the ethanol oxidative damage model at the end of experiment. The body weight of rats was measured. The contents of MDA, Protein carbonyl (PC), SOD, GSH and GSH-PX in hepatic tissue and blood serum were detected. Results The results showed there were no significant differences in the body weight among the three GSPPC groups, normal control and model control(P>0.05). Compared with the model control group, the content of MDA in low dosage of the GSPPC group was decreased in blood serum and hepatic tissue(P<0.05), and the content of GSH-PX was increased in blood serum and hepatic tissue (P<0.05).Compared with the model control group, the contents of GSH, SOD and GSH-PX were increased in blood serum and hepatic tissue(P<0.05, P<0.01), the contents of MDA and PC in middle and high dosage of the GSPPC group were decreased in hepatic tissue and blood serum(P<0.05, P<0.01). Conclusion The GSPPC showed significantly antioxidant effect.

Experimental study on anti-oxidative effect of proanthocyanidins phospholipid complex in grape seed

1. Department of Pharmacy, the First People s Hospital of Xiaoshan, Hangzhou 311200, China

2. Department of Pharmacy, Zhejiang Xiaoshan Hospital, Hangzhou 311201, China

Received Date: 2013-04-17

Rev Recd Date: 2013-06-28

Key words:

Abstract: Objective To study the antioxidant effects of grape seed proanthocyanidins phospholipid complex(GSPPC). Methods Eighty rats were randomly divided into eight groups: normal control, model control, Proanthocyanidins, Soybean phospholipids, Vitamin C and low dose of GSPPC, moderate dose of GSPPC, and high dose of GSPPC. Drugs were given orally to rats once a day for 30 days. And then, 50% ethanol was given to rats once by oral gavages to make the ethanol oxidative damage model at the end of experiment. The body weight of rats was measured. The contents of MDA, Protein carbonyl (PC), SOD, GSH and GSH-PX in hepatic tissue and blood serum were detected. Results The results showed there were no significant differences in the body weight among the three GSPPC groups, normal control and model control(P>0.05). Compared with the model control group, the content of MDA in low dosage of the GSPPC group was decreased in blood serum and hepatic tissue(P<0.05), and the content of GSH-PX was increased in blood serum and hepatic tissue (P<0.05).Compared with the model control group, the contents of GSH, SOD and GSH-PX were increased in blood serum and hepatic tissue(P<0.05, P<0.01), the contents of MDA and PC in middle and high dosage of the GSPPC group were decreased in hepatic tissue and blood serum(P<0.05, P<0.01). Conclusion The GSPPC showed significantly antioxidant effect.

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Experimental study on anti-oxidative effect of proanthocyanidins phospholipid complex in grape seed

doi: 10.3969/j.issn.1006-0111.2013.05.012

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