Differential proteomics analysis of human keratinocytes with transient receptor potential vanilloid-1 activated by capsaicin

HUANG Jin; WANG Jian; YUAN Fang; HU Wen-juan

doi:10.3969/j.issn.1006-0111.2013.05.004

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Objective To evaluate the differential proteomics of human keratinocytes with transient receptor potential vanilloid-1(TRPV1) activated by capsaicin. Methods Human keratinocytes were cultured and treated by capsaicin. Two-dimensional polyacrylamide gel electrophoresis(2D-PAGE) combined with MALDI-TOF/TOF identification were used to investigate the effect of TRPV1 activation on the proteome of keratinocytes. Results Compared with blank control, triosephosphate isomerase(TPI), enolase 1,6-phosphogluconolactonase, cathepsin D had higher expression in peritoneal macrophages of experimental endometriosis, while keratin 14 and 60 S acidic ribosomal protein P2 expressed lower. Conclusion It was revealed that there was a potential mechanism of TRPV1 activation in keratinocytes, which gave a new sight to find targets and screen drugs in skin disorders.

Differential proteomics analysis of human keratinocytes with transient receptor potential vanilloid-1 activated by capsaicin

Department Pharmacy, Pudong New Aera People's Hospital, Shanghai 201200, China

Received Date: 2013-06-07

Rev Recd Date: 2013-07-10

Key words:

transient receptor potential vanilloid-1 /
human keratinocytes /
capsaicin /
two-dimensional polyaerylamide gel electrophoresis

Abstract: Objective To evaluate the differential proteomics of human keratinocytes with transient receptor potential vanilloid-1(TRPV1) activated by capsaicin. Methods Human keratinocytes were cultured and treated by capsaicin. Two-dimensional polyacrylamide gel electrophoresis(2D-PAGE) combined with MALDI-TOF/TOF identification were used to investigate the effect of TRPV1 activation on the proteome of keratinocytes. Results Compared with blank control, triosephosphate isomerase(TPI), enolase 1,6-phosphogluconolactonase, cathepsin D had higher expression in peritoneal macrophages of experimental endometriosis, while keratin 14 and 60 S acidic ribosomal protein P2 expressed lower. Conclusion It was revealed that there was a potential mechanism of TRPV1 activation in keratinocytes, which gave a new sight to find targets and screen drugs in skin disorders.

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Reference (18)

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Differential proteomics analysis of human keratinocytes with transient receptor potential vanilloid-1 activated by capsaicin

doi: 10.3969/j.issn.1006-0111.2013.05.004

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