Simultaneous determination of contents of four components in three different dosage forms of Liuweidihuang by HPLC

Key words: 
• Liuweidihuang /
• HPLC /
• content determination /
• gallic acid /
• loganin /
• paeoniflorin /
• paeonol

Abstract: Objective To develop a new high performance liquid chromatography (HPLC) method for simultaneous determination of four major components (gallic acid, loganin, paeoniflorin and paeonol) in different dosage forms of Liuweidihuang. Methods The chromatography condition was with Agilent Zorbax SB-C₁₈ column (4.6×250 mm, 5 μm);mobile phase was A:ACN, B:0.1% formic acid, gradient elution, 0~3 min, A:3%~5%;3~18 min, A:5%~22%;18~60 min, A:22%~60%, flow speed was 1.0 ml/min, temperature of column was 25℃, inject volume was 10 μl, detection wavelength was 240 nm. Results The linearity was obtained over 6.296~318.9 μg/ml(r=0.999 8)for gallic acid, 1.952~99.04 μg/ml(r=0.999 7)for loganin, 6.186~309.3 μg/ml(r=0.999 8)for paeoniflorin and 7.147~214.4 μg/ml(r=0.999 7)for paeonol.The RSDs of precision of the samples were both less than 2%.The average recovery was between 98.2%~102.3%. Conclusion The present method, with satisfactory efficacy, was accurate and simple which could simultaneously determine four components, and could be used for quality control of different dosage forum of Liuweidihuang.