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Volume 31 Issue 4
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2013  >  31(4): 286-289

LI Sha, WU Wen-ying, XU Xiao-na, LI Xing-hua, MA Xiao-ya. Optimization of preparation of matrine liposome in ammonium sulfate gradient method by orthogonal experiment[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 286-289. doi: 10.3969/j.issn.1006-0111.2013.04.013
Citation: LI Sha, WU Wen-ying, XU Xiao-na, LI Xing-hua, MA Xiao-ya. Optimization of preparation of matrine liposome in ammonium sulfate gradient method by orthogonal experiment[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 286-289. doi: 10.3969/j.issn.1006-0111.2013.04.013
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Optimization of preparation of matrine liposome in ammonium sulfate gradient method by orthogonal experiment

doi: 10.3969/j.issn.1006-0111.2013.04.013
  • 1.

    Department of Pharmacy, School of Medicine, Xi'an Jiaotong University, Xian 710061, China

  • 2.

    Department of Pharmacy, the Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xian 710004, China

  • Received Date: 2012-09-05
  • Rev Recd Date: 2012-11-28
  • Objective To study the preparation method of matrine liposome and screen the optimal technological conditions by the encapsulation efficiencies. Methods Liposome was made of hydrogenated soybean phosphatidylcholine (HSPC) and cholesterol (Ch) by film ultrasonic-ammonium sulfate gradient method. Then orthogonal design was adopted to screen the optimal conditions. Free drug was separated by Sephadex gel and the encapsulation efficiency of matrine was determined by HPLC. Results The optimum conditions of matrine liposomes were as follows:HSPC:Ch=3:1, the time of probe ultrassonic was 10min, and the ratio of drug to lipid was 1:15. The average entrapment efficiency was 50.68%. Conclusions The entrapment efficiency of liposome could be obviously improved with the optimized condition. The method discussed above was simple, feasible, and easily operated, which could be suitable for laboratory to prepare matrine liposome.
  • [1] 刘 梅,刘雪英,程建峰.苦参碱的药理研究进展[J].中国中药杂志, 2003, 28(9):801.
    [2] Amarnath S, Uma SS. Liposomes in drug delivery:progress and limitation[J]. Int J Pharm, 1997(154):123.
    [3] 陈 浩,戴俊东,王玉蓉,等.薄膜超声法制备槲皮素脂质体研究[J].药学实践杂志,2012, 30(1):32.
    [4] 陆 彬.药物新剂型与新技术[M]. 北京:人民卫生出版社, 1998:120.
    [5] 王绍宁,邓意辉,严 志,等.硫酸铵梯度法制备盐酸环丙沙星脂质体的影响因素[J].沈阳药科大学学报, 2003, 20(2):94.
    [6] 黄 洁,仵文英,席枝侠,等.苦参碱脂质体的制备与包封率测定[J].医药导报,2008,27(1):87.
    [7] 仵文英,黄 洁,刘 硕,等.苦参碱隐形脂质体的制备及影响因素考察[J].中国医院药学杂志,2008,28(21):1816.
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Optimization of preparation of matrine liposome in ammonium sulfate gradient method by orthogonal experiment

doi: 10.3969/j.issn.1006-0111.2013.04.013
  • 1. Department of Pharmacy, School of Medicine, Xi'an Jiaotong University, Xian 710061, China
  • 2. Department of Pharmacy, the Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xian 710004, China
  • Received Date: 2012-09-05
  • Rev Recd Date: 2012-11-28

Abstract: Objective To study the preparation method of matrine liposome and screen the optimal technological conditions by the encapsulation efficiencies. Methods Liposome was made of hydrogenated soybean phosphatidylcholine (HSPC) and cholesterol (Ch) by film ultrasonic-ammonium sulfate gradient method. Then orthogonal design was adopted to screen the optimal conditions. Free drug was separated by Sephadex gel and the encapsulation efficiency of matrine was determined by HPLC. Results The optimum conditions of matrine liposomes were as follows:HSPC:Ch=3:1, the time of probe ultrassonic was 10min, and the ratio of drug to lipid was 1:15. The average entrapment efficiency was 50.68%. Conclusions The entrapment efficiency of liposome could be obviously improved with the optimized condition. The method discussed above was simple, feasible, and easily operated, which could be suitable for laboratory to prepare matrine liposome.

LI Sha, WU Wen-ying, XU Xiao-na, LI Xing-hua, MA Xiao-ya. Optimization of preparation of matrine liposome in ammonium sulfate gradient method by orthogonal experiment[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 286-289. doi: 10.3969/j.issn.1006-0111.2013.04.013
Citation: LI Sha, WU Wen-ying, XU Xiao-na, LI Xing-hua, MA Xiao-ya. Optimization of preparation of matrine liposome in ammonium sulfate gradient method by orthogonal experiment[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 286-289. doi: 10.3969/j.issn.1006-0111.2013.04.013
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