XIANG Yan-ying, MING Qian-liang, LI Wen-chao, HAN Ting, QIN Lu-ping. Seasonal dynamics and tissue distribution of endophytic fungi in Cephalotaxus fortunei Hook. f.[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 267-270,289. doi: 10.3969/j.issn.1006-0111.2013.04.007
| Citation:
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XIANG Yan-ying, MING Qian-liang, LI Wen-chao, HAN Ting, QIN Lu-ping. Seasonal dynamics and tissue distribution of endophytic fungi in Cephalotaxus fortunei Hook. f.[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(4): 267-270,289. doi: 10.3969/j.issn.1006-0111.2013.04.007
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Seasonal dynamics and tissue distribution of endophytic fungi in Cephalotaxus fortunei Hook. f.
- 1.
Department of Pharmacy, The 161th Hospital of PLA, Wuhan 430010, China
- 2.
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- Received Date: 2013-01-16
- Rev Recd Date:
2013-04-05
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Abstract
Objective To investigated the species composition, number and distribution of endophytes in medicinal plants Cephalotaxus fortunei Hook. f. in different seasons and different tissues. Methods Endophytic fungi were isolated by tissue-culture method and identified by ITS analysis. Results 1851 strains of endophytic fungi were isolated from the leaves, twigs and barks of C. fortunei which were sampled in spring, summer, autumn and winter. These stains were divided into 77 morphotype according to the cultural characteristics and were identified as 70 taxons by ITS analysis. The results suggested that effects of season and tissue on the colonization rate, isolation rate and species composition of endophytes were apparent, and effects of season were stronger than that of tissue. Conclusion There are abundant and multiple endophytie fungi in Cephalotaxus fortunei. According to the comprehensive analysis combined with richness and biodiversity indexes, spring was the best sampling time for getting most species of endophytes.
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