Determination of tectoridin and tectorigenin in <em>Flos Puerariae lobata</em> by HPLC

ZHAO Nan; LIU Dan; WANG Yan-ping; WANG Jin-feng; WEI Ying

doi:10.3969/j.issn.1006-0111.2012.03.020

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Objective To establish an HPLC method for the determination of Tectoridin and Tectorigenin in Flos Puerariae lobata. Method The separation was performed on Agilent -C₁₈ column(4.6 mm×250 mm,5 μm),using a mobile phase with methanol-acetonitrile-water(2∶1∶2). Flow rate was 1.0ml/min,the detection wavelength was set at 264 nm. Column temperature was ambient temperature. Result The linear range of tectoridin was 2.0 ~120.0 μg/ml,r=0.999 8.The average recovery was 99.7%,RSD was 1.9%;The linear range of tectorigenin was 0.8 ~80.0 μg/ml,r=0.998 6.The average recovery was 99.4%,RSD was 1.7%. Conclusion The method was simple ,accurate,reliable with a good repeatability,which could be used to determinate of tectoridin and tectorigenin in Flos Puerariae lobata.

Determination of tectoridin and tectorigenin in Flos Puerariae lobata by HPLC

The No.208 Hospital of PLA,Changchun 130062,China

Received Date: 2012-01-10

Rev Recd Date: 2012-04-06

Key words:

Abstract: Objective To establish an HPLC method for the determination of Tectoridin and Tectorigenin in Flos Puerariae lobata. Method The separation was performed on Agilent -C₁₈ column(4.6 mm×250 mm,5 μm),using a mobile phase with methanol-acetonitrile-water(2∶1∶2). Flow rate was 1.0ml/min,the detection wavelength was set at 264 nm. Column temperature was ambient temperature. Result The linear range of tectoridin was 2.0 ~120.0 μg/ml,r=0.999 8.The average recovery was 99.7%,RSD was 1.9%;The linear range of tectorigenin was 0.8 ~80.0 μg/ml,r=0.998 6.The average recovery was 99.4%,RSD was 1.7%. Conclusion The method was simple ,accurate,reliable with a good repeatability,which could be used to determinate of tectoridin and tectorigenin in Flos Puerariae lobata.

HTML

Reference (7)

[1]	田代华.实用中药辞典.下卷[M].北京.人民卫生出版社,2002:1338,1897.
[2]	尹俊亭,仲英,孙敬勇,等.葛花化学成分的研究[J].中草药,2006,37(3):350.
[3]	张淑萍,贺云,刘柏涛,等.RP-HPLC测定野葛花中3种异黄酮[J].分析实验室,2006,25(2):74.
[4]	张国峰,吴瑕,白雪,等.HPLC法同时测定野葛花中4种异黄酮成分的含量[J].沈阳药科大学学报,2009,26(1):40.
[5]	刘立辉,李卫民,高英. HPLC同时测定野葛花中鸢尾黄素-7-O-木糖葡萄糖糖苷、鸢尾苷、鸢尾苷元的含量[J].中国中药杂志,2010,35(17):2308.
[6]	Kim CS,Shin SM,Ha HK,et al. Study of substance change in flowers of Pueraria thunbergiana B enth. during storage[J].Arch Pharm Res,2003,26(3):210.
[7]	Niiho Y, Nakajima Y, Yamazaki T, et al. Simultaneous analysis of isoflavones and saponins in Pueraria flowers using hplc coupled to an evaporative light scattering detector and isolation of a new isoflavone diglucoside[J]. J Nat Med, 2010,64(3):313.

[1]	田代华.实用中药辞典.下卷[M].北京.人民卫生出版社,2002:1338,1897.
[2]	尹俊亭,仲英,孙敬勇,等.葛花化学成分的研究[J].中草药,2006,37(3):350.
[3]	张淑萍,贺云,刘柏涛,等.RP-HPLC测定野葛花中3种异黄酮[J].分析实验室,2006,25(2):74.
[4]	张国峰,吴瑕,白雪,等.HPLC法同时测定野葛花中4种异黄酮成分的含量[J].沈阳药科大学学报,2009,26(1):40.
[5]	刘立辉,李卫民,高英. HPLC同时测定野葛花中鸢尾黄素-7-O-木糖葡萄糖糖苷、鸢尾苷、鸢尾苷元的含量[J].中国中药杂志,2010,35(17):2308.
[6]	Kim CS,Shin SM,Ha HK,et al. Study of substance change in flowers of Pueraria thunbergiana B enth. during storage[J].Arch Pharm Res,2003,26(3):210.
[7]	Niiho Y, Nakajima Y, Yamazaki T, et al. Simultaneous analysis of isoflavones and saponins in Pueraria flowers using hplc coupled to an evaporative light scattering detector and isolation of a new isoflavone diglucoside[J]. J Nat Med, 2010,64(3):313.

Message Board

Determination of tectoridin and tectorigenin in Flos Puerariae lobata by HPLC

doi: 10.3969/j.issn.1006-0111.2012.03.020

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Related

Proportional views

Determination of tectoridin and tectorigenin in Flos Puerariae lobata by HPLC

doi: 10.3969/j.issn.1006-0111.2012.03.020

The No.208 Hospital of PLA,Changchun 130062,China

HTML

Catalog

Message Board

Determination of tectoridin and tectorigenin in Flos Puerariae lobata by HPLC

doi: 10.3969/j.issn.1006-0111.2012.03.020

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Related

Proportional views

Determination of tectoridin and tectorigenin in Flos Puerariae lobata by HPLC

doi: 10.3969/j.issn.1006-0111.2012.03.020

The No.208 Hospital of PLA,Changchun 130062,China

HTML

Catalog

Export File

Citation

Format

Content