Determination of bacterial endotoxin in human coagulation factor Ⅷ by kinetic turbidimetric limulus test

SHENG Feng-xian; XIONG Ke-qiang; WANG Hui-zhi; ZHANG You-jie; GAO Li-ping

doi:10.3969/j.issn.1006-0111.2012.02.008

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Volume 30 Issue 2

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Article Navigation > Journal of Pharmaceutical Practice and Service > 2012 > 30(2): 107-108,114

SHENG Feng-xian, XIONG Ke-qiang, WANG Hui-zhi, ZHANG You-jie, GAO Li-ping. Determination of bacterial endotoxin in human coagulation factor Ⅷ by kinetic turbidimetric limulus test[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(2): 107-108,114. doi: 10.3969/j.issn.1006-0111.2012.02.008

Citation:

SHENG Feng-xian, XIONG Ke-qiang, WANG Hui-zhi, ZHANG You-jie, GAO Li-ping. Determination of bacterial endotoxin in human coagulation factor Ⅷ by kinetic turbidimetric limulus test[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(2): 107-108,114. doi: 10.3969/j.issn.1006-0111.2012.02.008

Determination of bacterial endotoxin in human coagulation factor Ⅷ by kinetic turbidimetric limulus test

doi: 10.3969/j.issn.1006-0111.2012.02.008

Shanghai Xinxing Medicine Co., Ltd., Shanghai 200135, China

Received Date: 2011-12-22
Rev Recd Date: 2012-03-06

Abstract

Objective To establish a method for determination of bacterial endotoxin in human coagulation factor Ⅷ by kinetic turbidimetric limulus test. Methods Based on interference experiment, the endotoxin was added in the samples, whose recovery should be within the range 50%~200%. Results The interference from limulus test was eliminated by diluting the Human coagulation factor Ⅷ (1→100 solution). Conclusions Turbidimetric-kinetic method was feasible, and had the same result with the rabbit-pyretogen test.
- human coagulation factor Ⅷ,
- bacterial endotoxin,
- kinetic turbidimetric limulus test,
- interference test

References

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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