WANG Jing, DONG Xin, ZOU Hao, JIN Lei, ZHANG Chuan. Determination of sodium Danshensu in rat liver by HPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 52-54. doi: 10.3969/j.issn.1006-0111.2012.01.014
| Citation:
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WANG Jing, DONG Xin, ZOU Hao, JIN Lei, ZHANG Chuan. Determination of sodium Danshensu in rat liver by HPLC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 52-54. doi: 10.3969/j.issn.1006-0111.2012.01.014
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Determination of sodium Danshensu in rat liver by HPLC-MS/MS
- 1.
New drug research center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;Deportment of pharmacy,Qingpu Distric Central Hosptal,Shanghai 201700, China
- 2.
Center of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- 3.
New drug research center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- Received Date: 2011-04-21
- Rev Recd Date:
2011-06-14
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Abstract
Objective To establish a HPLC-MS/MS method for determination of sodium Danshensu in SD rat liver. Methods Tissue samples were pretreated by liquid-liquid extraction method after homogenate, and ketoprofen was used as the internal standard. Analytical column was Diamonsil C18 (200 mm×4.6 mm, 5 μm). The mobile phase was methanol: water(0.01‰ formic acid)=80:20 with flow rate as 1.0 ml/min. Mass spectrum determination was performed in the MRM mode with target ions m/z 197→135 (Sodium Danshensu), m/z 253→209 (ketoprofen), using ESI ion resource and negative ion detection. Results The calibration curve was linear over the range of 20.2~1 010 ng/ml. The LLOQ of sodium Danshensu in liver was 20.2 ng/ml. The intra-and inter-day RSD were both less than 10%. The extracted recovery rate ranged from 92% to 107%. Conclusion The method was sensitive, rapid, simple and accurate to determine sodium Danshensu distribution in rat liver and was suitable for preclinical test of Sodium Danshensu.
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