Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat

CHEN Qian; CHEN Meng-li; YE Zhao-bo

doi:10.3969/j.issn.1006-0111.2012.01.006

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Objective To investigate the protect effect and mechanism of total polysaccharides (TPC) isolated from 50% ethanol extract of Wuzi-Yanzong pill (WZ) against chronic alcohol-induced injury in Rat . Methods The effect of TPC on chronic ethanol hepatotoxicity was investigated in modified Lieber-DeCarli rat model. Hepatocellular damage indicator (Serum ALT, AST) and histopathological examination of liver in rat were evaluated. MDA content, SOD activity and GSH content in liver were measured. TNF-α content and TNF-α mRNA expression were determined. Results TPC protected against chronic ethanol-induced hepatotoxicity in rat, as evidenced by decreased plasma transaminases activities and signally improve the liver pathological changes induced by alcohol. TPC significantly inhibited the increase of MDA content, improved SOD activity and prevented GSH depletion. TPC significantly alleviated hepatic TNF-α production and decreased the expression of TNF-α mRNA. Conclusion TPC possessed potent hepatoprotective activities in chronic alcohol-induced injury rat model. The suppressive effect of TPC on chronic alcohol induced liver injury might be attributed to the antioxidant action and suppress TNF-α expression at protein and gene level.

Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat

1. Department of clinical pharmacology research, General Hospital of PLA, Beijing 100853 China

2. College of traditional Chinese medicine , The Chinese University of Hong Kong , Hong Kong,China

Received Date: 2011-08-11

Rev Recd Date: 2011-10-14

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Abstract: Objective To investigate the protect effect and mechanism of total polysaccharides (TPC) isolated from 50% ethanol extract of Wuzi-Yanzong pill (WZ) against chronic alcohol-induced injury in Rat . Methods The effect of TPC on chronic ethanol hepatotoxicity was investigated in modified Lieber-DeCarli rat model. Hepatocellular damage indicator (Serum ALT, AST) and histopathological examination of liver in rat were evaluated. MDA content, SOD activity and GSH content in liver were measured. TNF-α content and TNF-α mRNA expression were determined. Results TPC protected against chronic ethanol-induced hepatotoxicity in rat, as evidenced by decreased plasma transaminases activities and signally improve the liver pathological changes induced by alcohol. TPC significantly inhibited the increase of MDA content, improved SOD activity and prevented GSH depletion. TPC significantly alleviated hepatic TNF-α production and decreased the expression of TNF-α mRNA. Conclusion TPC possessed potent hepatoprotective activities in chronic alcohol-induced injury rat model. The suppressive effect of TPC on chronic alcohol induced liver injury might be attributed to the antioxidant action and suppress TNF-α expression at protein and gene level.

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Reference (13)

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Protective effect of total polysaccharides in Wuzi-Yanzong pill on chronic alcohol-induced injury in Rat

doi: 10.3969/j.issn.1006-0111.2012.01.006

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