半枝莲和白花蛇舌草药对中总黄酮的提取及纯化工艺研究

王桂玲; 房建强; 费洪荣; 赵莹

doi:10.3969/j.issn.1006-0111.2019.06.013

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半枝莲和白花蛇舌草药对中总黄酮的提取及纯化工艺研究

王桂玲, 房建强, 费洪荣, 赵莹

文章导航 > 药学实践与服务 > 2019 > 37(6): 537-542

王桂玲, 房建强, 费洪荣, 赵莹. 半枝莲和白花蛇舌草药对中总黄酮的提取及纯化工艺研究[J]. 药学实践与服务, 2019, 37(6): 537-542. doi: 10.3969/j.issn.1006-0111.2019.06.013

引用本文:

WANG Guiling, FANG Jianqiang, FEI Hongrong, ZHAO Ying. Extraction and purification of total flavonoids from the herb pair of Scutellariae barbatae and Hedyotis diffusa[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 537-542. doi: 10.3969/j.issn.1006-0111.2019.06.013

Citation:

WANG Guiling, FANG Jianqiang, FEI Hongrong, ZHAO Ying. Extraction and purification of total flavonoids from the herb pair of Scutellariae barbatae and Hedyotis diffusa[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 537-542. doi: 10.3969/j.issn.1006-0111.2019.06.013

半枝莲和白花蛇舌草药对中总黄酮的提取及纯化工艺研究

doi: 10.3969/j.issn.1006-0111.2019.06.013

山东第一医科大学山东省医学科学院, 山东泰安 271016

基金项目: 2016年山东省高等学校科技计划项目（J16LM03）

Extraction and purification of total flavonoids from the herb pair of Scutellariae barbatae and Hedyotis diffusa

Shandong First Medical University&Shandong Academy of Medical Sciences, Taian 271016, China

摘要: 目的筛选出半枝莲和白花蛇舌草药对中总黄酮提取及纯化的最佳工艺条件。方法以总黄酮提取率和浸膏量为评价指标，选择两者配比、乙醇体积分数、料液比、提取时间4个因素作为考察对象，L₉（3⁴）正交试验优选总黄酮提取工艺。然后结合静态和动态实验方法，以吸附率、解吸率为指标，确定聚酰胺树脂纯化的工艺条件及参数。结果确定药对中总黄酮提取的工艺条件：半枝莲与白花蛇舌草药对的配比为4：1，25倍量体积分数为70%的乙醇，提取3次，每次2 h。聚酰胺树脂纯化药对中总黄酮的工艺条件：上样液中总黄酮浓度为63.14 mg/ml，pH为4.0，以1.5 ml/min的流速上样，先用流速为3 ml/min的3.1床容积（BV）的水洗脱，再用流速为3 ml/min的9.3 BV体积分数为65%的乙醇洗脱，收集洗脱液，测得总黄酮纯度为40.39%，转移率为81.57%。结论优化的提取工艺条件稳定、可行，黄酮提取率较高。聚酰胺树脂纯化的工艺条件简便，效果良好。
- 半枝莲 /
- 白花蛇舌草 /
- 总黄酮 /
- 正交试验 /
- 聚酰胺树脂
Abstract: Objective To screen out the optimal conditions for extraction and purification of total flavonoids from the herb pair of Scutellariae barbatae and Hedyotis diffusa. Methods Single factor and L₉(3⁴) orthogonal experiment was designed with the extraction ratio and extract quantity of total flavonoids as indexes,using matching of herb pair,ethanol concentration,solid-liquid ratio,extraction time as inspected elements.The technical process for purification of total flavonoids with the static and dynamic adsorption experiments was screened by adsorption and desorption ratio as targets. Results The optimal process conditions were:ethanol concentration 70%,the matching of herb pair of 4:1,the solid-liquid ratio of 1:25,extraction for 3 times and 2h for each time.The parameters of the purification process were as follows:concentration of sample 63.14 mg/ml and pH value 4.0,which used in the chromatographic column of polyamide resin at a flow velocity of 1.5 ml/min.The chromatographic column was eluted with purified water of 3.1BV,and then eluted with 65% ethanol concentration of 9.3BV at a flow velocity of 3ml/min.The purity of the total flavonoids was 40.39% in the fine product,and the transfer rate was 81.57%. Conclusion The optimal process conditions were reasonable and feasible.The purification technique of polyamide resin was uncomplicated and had a good effect.
- Scutellaria barbata D.Don /
- Hedyotis diffusa Willd /
- total flavonoids /
- orthogonal experiment /
- polyamide resin

[1]	彭献娜,刘艺华,刘亚林,等.半枝莲化学成分和药理活性研究进展[J].亚太传统医药,2014,10(17):57-58.
[2]	陈玉,冯大刚,胡荣,等.半枝莲和白花蛇舌草总多糖对S180荷瘤小鼠的抗肿瘤作用研究[J].新中医,2013,45(5):171-174.
[3]	纪宝玉,范崇庆,裴莉昕,等.白花蛇舌草的化学成分及药理作用研究进展[J].中国实验方剂学杂志,2014,20(19):235-240.
[4]	张秋梅,孙增玉.白花蛇舌草化学成分研究[J].中药材,2014,37(12):2216-2218.
[5]	李柳叶,邢玉,包晓霞,等.白花蛇舌草黄酮和多糖对与肿瘤相关巨噬细胞共培养的人子宫内膜癌Ishikawa细胞的影响[J].中国妇产科临床杂志,2015,16(2):150-153.
[6]	史玉荣,徐海波,石梦莹,等.白花蛇舌草通过Wnt信号通路抑制结肠肿瘤干细胞分化[J].中药药理与临床,2015,31(1):133-136.
[7]	李伟霞,唐于平,刘立,等.药对研究(Ⅲ):药对的功效物质基础[J].中国中药杂志,2013,38(24):4196-4202.
[8]	杨鸿珠,龚雨萍.中药药对的研究进展与思考[J].中国中西医结合杂志,2010,30(2):218-220.
[9]	邓树泳,李静,罗来成.中药药对的现代研究进展[J].时珍国医国药,2012,23(4):1003-1005.
[10]	王桂玲,房建强,赵雪梅,等.拳参总黄酮的纯化研究[J].医药导报,2011,30(2):190-193.
[11]	李梓盟,张柯达,吴金虎.聚酰胺树脂纯化复方"前愈"总黄酮的工艺研究[J].中国新药杂志,2016,25(8):913-916.
[12]	周光姣,孙宜坤,王超群.鸡血藤中总黄酮的聚酰胺树脂纯化工艺优选[J].中国实验方剂学杂志,2014,20(12):34-37.

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半枝莲和白花蛇舌草药对中总黄酮的提取及纯化工艺研究

doi: 10.3969/j.issn.1006-0111.2019.06.013

山东第一医科大学山东省医学科学院, 山东泰安 271016

基金项目: 2016年山东省高等学校科技计划项目（J16LM03）

收稿日期: 2018-09-19
修回日期: 2019-11-04

关键词:

半枝莲 /

白花蛇舌草 /

总黄酮 /

正交试验 /

聚酰胺树脂

摘要: 目的筛选出半枝莲和白花蛇舌草药对中总黄酮提取及纯化的最佳工艺条件。方法以总黄酮提取率和浸膏量为评价指标，选择两者配比、乙醇体积分数、料液比、提取时间4个因素作为考察对象，L₉（3⁴）正交试验优选总黄酮提取工艺。然后结合静态和动态实验方法，以吸附率、解吸率为指标，确定聚酰胺树脂纯化的工艺条件及参数。结果确定药对中总黄酮提取的工艺条件：半枝莲与白花蛇舌草药对的配比为4：1，25倍量体积分数为70%的乙醇，提取3次，每次2 h。聚酰胺树脂纯化药对中总黄酮的工艺条件：上样液中总黄酮浓度为63.14 mg/ml，pH为4.0，以1.5 ml/min的流速上样，先用流速为3 ml/min的3.1床容积（BV）的水洗脱，再用流速为3 ml/min的9.3 BV体积分数为65%的乙醇洗脱，收集洗脱液，测得总黄酮纯度为40.39%，转移率为81.57%。结论优化的提取工艺条件稳定、可行，黄酮提取率较高。聚酰胺树脂纯化的工艺条件简便，效果良好。

English Abstract

Extraction and purification of total flavonoids from the herb pair of Scutellariae barbatae and Hedyotis diffusa

Shandong First Medical University&Shandong Academy of Medical Sciences, Taian 271016, China

Received Date: 2018-09-19
Rev Recd Date: 2019-11-04

Keywords:

Abstract: Objective To screen out the optimal conditions for extraction and purification of total flavonoids from the herb pair of Scutellariae barbatae and Hedyotis diffusa. Methods Single factor and L₉(3⁴) orthogonal experiment was designed with the extraction ratio and extract quantity of total flavonoids as indexes,using matching of herb pair,ethanol concentration,solid-liquid ratio,extraction time as inspected elements.The technical process for purification of total flavonoids with the static and dynamic adsorption experiments was screened by adsorption and desorption ratio as targets. Results The optimal process conditions were:ethanol concentration 70%,the matching of herb pair of 4:1,the solid-liquid ratio of 1:25,extraction for 3 times and 2h for each time.The parameters of the purification process were as follows:concentration of sample 63.14 mg/ml and pH value 4.0,which used in the chromatographic column of polyamide resin at a flow velocity of 1.5 ml/min.The chromatographic column was eluted with purified water of 3.1BV,and then eluted with 65% ethanol concentration of 9.3BV at a flow velocity of 3ml/min.The purity of the total flavonoids was 40.39% in the fine product,and the transfer rate was 81.57%. Conclusion The optimal process conditions were reasonable and feasible.The purification technique of polyamide resin was uncomplicated and had a good effect.