升陷汤及各单味药对阿霉素致心肌细胞损伤的保护作用

满缓; 张凤; 黄豆豆; 熊筱娟; 詹勤; 廖丽娜; 李铁军; 陈万生

doi:10.3969/j.issn.1006-0111.2019.04.004

升陷汤及各单味药对阿霉素致心肌细胞损伤的保护作用

doi: 10.3969/j.issn.1006-0111.2019.04.004

1.
海军军医大学附属长征医院药材科, 上海 200003;宜春学院化学与生物工程学院, 江西宜春 336000
2.
海军军医大学附属长征医院药材科, 上海 200003
3.
宜春学院化学与生物工程学院, 江西宜春 336000
4.
海军军医大学药学院药理教研室, 上海 200433

基金项目: 国家自然科学基金（81470173），上海市科技成果转化和产业化项目（18401931600）

Protective effect of Shengxian decoction and each single herb component against adriamycin-induced cardiomyocyte injury

1.
Department of Medicine, Changzheng Hospital, Naval Medical University, Shanghai 200003, China;College of Chemical and Biological Engineering, Yichun University, Yichun 336000, China
2.
Department of Medicine, Changzheng Hospital, Naval Medical University, Shanghai 200003, China
3.
College of Chemical and Biological Engineering, Yichun University, Yichun 336000, China
4.
Department of Pharmacology, College of Pharmacy, Naval Medical University, Shanghai 200433, China

摘要: 目的研究升陷汤（SXT）及各单味药对阿霉素致心肌细胞损伤的保护作用。方法将乳鼠缺氧/复氧培养的心肌细胞分为正常对照组（Con组）、阿霉素诱导损伤组（M组）和不同药物（升陷汤及其各单味药）治疗阿霉素诱导损伤组（SXT、A-E组）。检测指标包括细胞活力、细胞活性氧族（ROS）、Ca²⁺和乳酸脱氢酶（LDH）水平。结果与正常组相比，阿霉素能够造成心肌细胞损伤，与模型组比较，升陷汤全方以及各单味药实验组均能增强细胞的活力，降低细胞Ca²⁺和LDH浓度；除桔梗和升麻外，升陷汤全方及其他单味药能够降低ROS的浓度，实验结果提示，在早期阿霉素治疗中如果应用升陷汤，一定程度上能降低阿霉素的心脏毒性作用。结论升陷汤及各单味药对阿霉素致心肌细胞损伤具有保护作用。
- 升陷汤 /
- 阿霉素 /
- 心肌细胞损伤 /
- 保护作用
Abstract: Objective To study the protective effect of Shengxian decoction (SXT) and its each single herb component against doxorubicin induced cardiomyocyte injury. Methods Myocardial cells isolated from the neonatal rats with hypoxia/reoxygenation injury of cardiomyocytes were divided into eight groups,control group (Con group),doxorubicin-induced injury group (M group),and treatment groups with Shengxian decoction and its each single herb decoction (SXT,A-E groups) following doxorubicin-induced injury.The indicators tested included cell viability,cell ROS,Ca²⁺ and LDH levels. Results Compared with the control group,doxorubicin induced obvious myocardial injury in model group.Compared with the model group,all tested drug groups increased the cell viability,reduced the levels of cell Ca²⁺ and LDH.Besides,SXT and the single herb decoction reduced the concentration of ROS except the decoction of Platycodon grandiflorum and Cimicifuage Rhizoma.The experimental results suggested that the application of SXT in the early episode could reduce the cardiac toxicity of adriamycin to a certain extent. Conclusion SXT and its each single herb decoction showed protective effect against doxorubicin-induced cardiomyocyte injury.
- Shengxian decoction /
- adriamycin /
- cardiomyocyte injury /
- protection effect

[1]	李雅静,王晨.左卡尼汀注射液对阿霉素所致心肌细胞损伤的保护作用[J].现代药物与临床,2013,28(1):18-20.
[2]	THORN C F,OSHIRO C,MARSH S,et al.Doxorubicin pathways:pharmacodynamics and adverse effects[J].Pharmacogenet Genomics,2011,21(7):440-446.
[3]	赵雅君,王伊林,陶谢鑫,等.阿霉素致心肌细胞凋亡时网腔钙结合蛋白的变化[J].临床心血管病杂志,2015,31(8):895-898.
[4]	郝刚,俞蕴莉.阿霉素心肌毒性机制研究进展[J].中南药学,2014,12(10):993-997.
[5]	CARVALHO PBD,GONCALVES ADF,ALEGRE PHC,et al.Pamidronate attenuates oxidative stress and energetic metabolism changes but worsens functional outcomes in acute doxorubicin-induced cardiotoxicity in rats[J].Cell Physiol Biochem.,2016,40(3-4):431.
[6]	VITELLI M R,FILIPPELLI A,RINALDI B,et al.Effects of docosahexaenoic acid on[Ca²⁺] i,increase induced by doxorubicin in ventricular rat cardiomyocytes[J].Life Sci,2002,71(16):1905-1916.
[7]	胡开永,杨勇,何莉华,等.五味子乙素和右丙亚胺对阿霉素诱导心脏毒性的保护作用[J].药学学报,2014,49(7):1007-1012.
[8]	王玉民.升陷汤应用心得[J].中医药临床杂志,2011,23(7):641-642.
[9]	詹勤.桔梗在升陷汤中引经作用及其化学成分研究[D].上海:第二军医大学, 2012.
[10]	杨承芝,朱爱华.浅析张锡纯大气下陷证与升陷汤[J].中国中医药现代远程教育,2014,12(14):116-117.
[11]	李庆.升陷汤治疗慢性心力衰竭30例临床观察[J].新中医,2013,45(3):29-30.
[12]	刘建伟,邹海丽.升陷汤在心血管疾病中的运用[J].中医研究,2015,28(2):41-43.
[13]	朱祖成.清热解毒中药对心肌细胞缺氧复氧损伤影响的实验研究[J].中国民族民间医药,2013,22(2):34-36.
[14]	王亚东,PATRICK,ASARE,等.大株红景天注射液对心肌细胞缺氧/复氧损伤的保护作用[J].天津中医药,2016(3):160-163.
[15]	郝刚,俞蕴莉.小檗碱对阿霉素诱导心肌细胞损伤的保护作用研究[J].中国实验方剂学杂志,2015,21(24):114-118.
[16]	TACAR O,SRIAMORNSAK P,DASS C R.Doxorubicin:an update on anticancer molecular action,toxicity and novel drug delivery systems[J].J Pharm Pharmacol,2013,65(2):157-170.
[17]	曹程浩,班高亚.王振涛运用升陷汤治疗心系疾病经验[J].河南中医,2014,34(2):249-250.
[18]	田耀博,赵大庆,李香艳,等.人参多糖通过抑制ROS水平和凋亡保护H₂O₂诱导的心肌细胞氧化应激损伤[J].华中师范大学学报(自然科学版),2018,52(2):240-247.
[19]	SLIMEN I B,NAJAR T,GHRAM A,et al.Reactive oxygen species,heat stress and oxidative-induced mitochondrial damage.A review[J].Int J Hyperthermia,2014,30(7):513-523.
[20]	韦淑娟,任志涛,杨信怡,等.昆布多糖硫酸酯对H₂O₂诱导乳鼠心肌细胞损伤的保护作用[J].中国海洋药物,2015(2):39-44.
[21]	ZHAO Y,GAO F,ZHANG Y,et al.ShensongYangxin capsules prevent ischemic arrhythmias by prolonging action potentials and alleviating Ca²⁺ overload[J].Mol Med Rep,2016,13(6):5185-5192.

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升陷汤及各单味药对阿霉素致心肌细胞损伤的保护作用

doi: 10.3969/j.issn.1006-0111.2019.04.004

Protective effect of Shengxian decoction and each single herb component against adriamycin-induced cardiomyocyte injury

计量

升陷汤及各单味药对阿霉素致心肌细胞损伤的保护作用

doi: 10.3969/j.issn.1006-0111.2019.04.004

English Abstract

Protective effect of Shengxian decoction and each single herb component against adriamycin-induced cardiomyocyte injury

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留言板

升陷汤及各单味药对阿霉素致心肌细胞损伤的保护作用

doi: 10.3969/j.issn.1006-0111.2019.04.004

Protective effect of Shengxian decoction and each single herb component against adriamycin-induced cardiomyocyte injury

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升陷汤及各单味药对阿霉素致心肌细胞损伤的保护作用

doi: 10.3969/j.issn.1006-0111.2019.04.004

English Abstract

Protective effect of Shengxian decoction and each single herb component against adriamycin-induced cardiomyocyte injury

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目录