Study on extraction process of Ligustrum lucidum Ait
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摘要: 目的 优化女贞子的最佳提取条件。 方法 采用正交试验法设计实验，考察乙醇浓度、加醇量、提取时间和提取次数，通过齐墩果酸、特女贞苷含量测定及浸膏得率来确定最佳乙醇提取工艺；考察粉碎度对女贞子提取工艺的影响，比较女贞子提取液趁热过滤浓缩与静置24 h后过滤浓缩的结果差异，并比较女贞子醇提工艺和水提工艺的各项指标。 结果 女贞子最佳提取工艺为加10倍量的70%乙醇，提取3次，每次1 h，提取液趁热过滤浓缩。 结论 该优化提取工艺合理可行，更适合生产。Abstract: Objective To optimized the ethanol extraction process of Ligustrum lucidum Ait. Methods L9(34) orthogonal experiment was adopted with the change of the contents of oleanolic acid,specnuezhenide and extraction yield.The best ethanol extraction process was optimized by concentration of ethanol,the amount of ethanol,extraction time and extraction times.The effects of the extraction process of Ligustrum lucidum Ait such as crushing degrees and different solvents were inspected in this experiment-.The extraction process was selected from the extraction which was filtered hot and concentrated or filtered and concentrated after standing for 24 h. Results The best extraction process of Ligustrum lucidum Ait was extracted in 10 times 70% ethanol solution for 3 times and heated for 1 hour per time,and then the extraction was filtered hot and concentrated. Conclusion The extraction process of Ligustrum lucidum Ait was reasonable which was more suitable for production.
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Key words:
- Ligustrum lucidum Ait /
- orthogonal test /
- oleanolic acid /
- specnuezhenide
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