用LC-MS/MS法测定大鼠血浆中大蒜皂苷PIEB的浓度及其体内药动学研究

陈浩; 李新方; 孙渺; 张钢; 杨峰

doi:10.3969/j.issn.1006-0111.2019.01.009

用LC-MS/MS法测定大鼠血浆中大蒜皂苷PIEB的浓度及其体内药动学研究

doi: 10.3969/j.issn.1006-0111.2019.01.009

陈浩¹,
李新方²,
孙渺²,
张钢³,
杨峰^2,

1.
解放军总医院海南分院药剂科, 海南三亚 572013
2.
海军军医大学药学院, 上海 200433
3.
陆军军医大学高原军事医学系高原医学教育部重点实验室, 重庆 400038

基金项目: 全军后勤科研计划重大项目（AWS14L005）

Study on determination and pharmacokinetics of garlic saponins PIEB in the rat plasma by LC-MS/MS

1.
Department of Pharmacy, Hainan Branch of PLA General Hospital, Sanya 572013, China
2.
School of Pharmacy, Naval Medical University, Shanghai 200433, China
3.
College of High Altitude Military Medicine, Army Medical University, Chongqing 400038, China

摘要: 目的采用高效液相色谱-质谱（LC-MS/MS）法测定大鼠血浆中大蒜皂苷提取物中大蒜皂苷Proto-iso-eruboside-B（PIEB）的浓度，并研究其药动学特点。方法采用Athena C₁₈-WP色谱柱（50 mm×2.1 mm，3 μm），流动相为乙腈-水（含0.05%甲酸）体系，梯度洗脱，流速0.3 ml/min。大鼠分别给予低、中、高剂量大蒜皂苷总提取物灌胃及静脉注射单一剂量大蒜皂苷总提取物，给药后在不同的时间点眼眶取血，采用LC-MS/MS法测定血药浓度；采用InnaPhase Kinetica2000^TM软件计算相关药动学参数。结果大蒜皂苷提取物中的PIEB成分在10~2 430 ng/ml范围内具有良好的线性关系（r=0.999 1）；方法学考察均符合要求；大鼠静脉给药大蒜皂苷总提取物（50 mg/kg）后，PIEB即刻在体内达到最大血药浓度，其t_1/2为322 min左右，平均驻留时间（MRT）为270 min；口服灌胃低、中、高浓度大蒜皂苷总提取物后，PIEB的AUC、c_max等随给药剂量的增加而增大，大蒜皂苷提取物中的PIEB成分在大鼠体内的药动学过程符合线性药动学特征。结论本方法操作简便、结果准确、重复性好，适用于测定大鼠血浆中大蒜皂苷PIEB的浓度。
- 大蒜皂苷PIEB /
- 高效液相色谱-质谱 /
- 药动学 /
- 含量测定
Abstract: Objective To establish a method for concentration determination of garlic saponins PIEB in the rat plasma and study the pharmacokinetics of PIEB. Methods The separation was performed on a reverse phase Athena C₁₈-WP (50 mm×2.1 mm, 3 μm), with the mobile phase consisted of elution of acetonitrile-water (contain 0.05% formic acid) at a flow rate of 0.3 ml/min.After intravenous and gavage administration, the blood samples of rats were collected in different time, and measured by LC-MS/MS.The pharmacokinetics parameters were calculated by Inna Phase Kinetica2000^TM pharmacokinetics software. Results There was a good linearity of the calibration curves over the concentration range of 10-2 430 ng/ml (r=0.999 1), and the investigation of methodology all accorded with the demands.After intravenous administration of 50 mg/kg PIEB in rats, the maximum concentration of PIEB was achieved immediately.The t_1/2 of PIEB was about 322 minutes and the MRT was 270 minutes.After oral administration of low, medium and high concentrations of garlic saponin, the AUC and c_max increased with the increasing of dosage, which were consistent with the features of linear pharmacokinetics. Conclusion The established method was simple, accurate and sensitive, which could be suitable for the contents determination of garlic saponins PIEB in the rat plasma.
- garlic saponins PIEB /
- LC-MS/MS /
- pharmacokinetics /
- content determination

[1]	XIAO J,XING F,LIU Y,et al.Garlic-derived compound S-allylmercaptocysteine inhibits hepatocarcinogenesis through targeting LRP6/Wnt pathway[J].Acta Pharm Sin B, 2018,8(4):575-586.
[2]	HASSANZADEH H,ALIZADEH M,REZAZAD BARI M. Formulation of garlic oil-in-water nanoemulsion:antimicrobial and physicochemical aspects[J].IET Nanobiotechnol,2018,12(5):647-652.
[3]	SHABANI E,SAYEMIRI K,MOHAMMADPOUR M.The effect of garlic on lipid profile and glucose parameters in diabetic patients:A systematic review and meta-analysis[J].Prim Care Diabet,2018.
[4]	KANG J S,KIM S O,KIM G Y,et al.An exploration of the antioxidant effects of garlic saponins in mouse-derived C2C12 myoblasts.[J].Int J Mol Med,2016,37(1):149-156.
[5]	周思敏,郑善军,张钢.大蒜总皂苷对小鼠耐缺氧作用的实验研究[J].中国病理生理杂志,2015(10):1897.
[6]	柏冬,王瑞海,刘丽梅.大蒜皂苷对照品制备及比色法测定大蒜皂苷提取物中总皂苷含量[J].中国中医药信息杂志,2012,19(9):55-58.
[7]	张家伟,王晓明,潘桂湘,等.UPLC-MS/MS法测定大鼠灌服稳心颗粒后血浆中3个皂苷成分及其药动学研究[J].辽宁中医杂志,2016(8):1717-1720.
[8]	宋兴良,梁恕坤,赵翊萌,等.柱前衍生化-HPLC法测定大蒜中甾体皂苷的含量[J].中国药房,2016,27(3):361-364.
[9]	周梦娣,何功浩,倪伟,等.LC-MS/MS法测定大鼠血浆中丫蕊花皂苷G的浓度及其体内药动学研究[J].华西药学杂志,2018,33(3):279-282.
[10]	刘继华,卢丹,刘金平,等.大鼠肌内注射20(S)-人参皂苷Rg3的药动学研究[J].中国药学杂志,2007,42(14):1087-1090.

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用LC-MS/MS法测定大鼠血浆中大蒜皂苷PIEB的浓度及其体内药动学研究

doi: 10.3969/j.issn.1006-0111.2019.01.009

Study on determination and pharmacokinetics of garlic saponins PIEB in the rat plasma by LC-MS/MS

计量

用LC-MS/MS法测定大鼠血浆中大蒜皂苷PIEB的浓度及其体内药动学研究

doi: 10.3969/j.issn.1006-0111.2019.01.009

1. 解放军总医院海南分院药剂科, 海南三亚 572013

2. 海军军医大学药学院, 上海 200433

3. 陆军军医大学高原军事医学系高原医学教育部重点实验室, 重庆 400038

English Abstract

Study on determination and pharmacokinetics of garlic saponins PIEB in the rat plasma by LC-MS/MS

1. Department of Pharmacy, Hainan Branch of PLA General Hospital, Sanya 572013, China

2. School of Pharmacy, Naval Medical University, Shanghai 200433, China

3. College of High Altitude Military Medicine, Army Medical University, Chongqing 400038, China

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用LC-MS/MS法测定大鼠血浆中大蒜皂苷PIEB的浓度及其体内药动学研究

doi: 10.3969/j.issn.1006-0111.2019.01.009

Study on determination and pharmacokinetics of garlic saponins PIEB in the rat plasma by LC-MS/MS

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用LC-MS/MS法测定大鼠血浆中大蒜皂苷PIEB的浓度及其体内药动学研究

doi: 10.3969/j.issn.1006-0111.2019.01.009

1. 解放军总医院海南分院药剂科, 海南 三亚 572013 2. 海军军医大学药学院, 上海 200433 3. 陆军军医大学高原军事医学系高原医学教育部重点实验室, 重庆 400038

English Abstract

Study on determination and pharmacokinetics of garlic saponins PIEB in the rat plasma by LC-MS/MS

1. Department of Pharmacy, Hainan Branch of PLA General Hospital, Sanya 572013, China 2. School of Pharmacy, Naval Medical University, Shanghai 200433, China 3. College of High Altitude Military Medicine, Army Medical University, Chongqing 400038, China

全文HTML

目录

1. 解放军总医院海南分院药剂科, 海南三亚 572013

2. 海军军医大学药学院, 上海 200433

3. 陆军军医大学高原军事医学系高原医学教育部重点实验室, 重庆 400038

1. Department of Pharmacy, Hainan Branch of PLA General Hospital, Sanya 572013, China

2. School of Pharmacy, Naval Medical University, Shanghai 200433, China

3. College of High Altitude Military Medicine, Army Medical University, Chongqing 400038, China