高效液相色谱法测定体外大鼠肠道菌液中大豆苷及其代谢物

张圳; 冯家懿; 果卉; 姚媛; 闻俊; 周婷婷

doi:10.3969/j.issn.1006-0111.2018.04.013

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

高效液相色谱法测定体外大鼠肠道菌液中大豆苷及其代谢物

张圳, 冯家懿, 果卉, 姚媛, 闻俊, 周婷婷

文章导航 > 药学实践与服务 > 2018 > 36(4): 347-350

张圳, 冯家懿, 果卉, 姚媛, 闻俊, 周婷婷. 高效液相色谱法测定体外大鼠肠道菌液中大豆苷及其代谢物[J]. 药学实践与服务, 2018, 36(4): 347-350. doi: 10.3969/j.issn.1006-0111.2018.04.013

引用本文:

ZHANG Zhen, FENG Jiayi, GUO Hui, YAO Yuan, WEN Jun, ZHOU Tingting. Simultaneously determination of daidzin and its metabolite in rat intestinal bacteria test solution in vitro by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(4): 347-350. doi: 10.3969/j.issn.1006-0111.2018.04.013

Citation:

ZHANG Zhen, FENG Jiayi, GUO Hui, YAO Yuan, WEN Jun, ZHOU Tingting. Simultaneously determination of daidzin and its metabolite in rat intestinal bacteria test solution in vitro by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(4): 347-350. doi: 10.3969/j.issn.1006-0111.2018.04.013

高效液相色谱法测定体外大鼠肠道菌液中大豆苷及其代谢物

doi: 10.3969/j.issn.1006-0111.2018.04.013

张圳¹,
冯家懿¹,
果卉¹,
姚媛²,
闻俊¹,
周婷婷^1,

1.
第二军医大学药学院, 上海市药物(中药)代谢产物研究重点实验室, 上海 200433
2.
第二军医大学药学院, 上海市药物(中药)代谢产物研究重点实验室, 上海 200433;安徽中医药大学药学院, 安徽合肥 230012

基金项目: 国家自然科学基金资助项目（81573584，81773862）；第二军医大学本科生创新孵化基地项目（FM2016071）

Simultaneously determination of daidzin and its metabolite in rat intestinal bacteria test solution in vitro by HPLC

1.
School of Pharmacy & Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Second Military Medical University Shanghai 200433, China
2.
School of Pharmacy & Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Second Military Medical University Shanghai 200433, China;School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China

摘要: 目的建立HPLC法测定体外大鼠肠道菌液中的大豆苷及其代谢物，研究大鼠肠道菌群对大豆苷的代谢情况。方法采用大鼠离体粪便温孵法，将大豆苷与大鼠肠道菌群培养液进行厌氧温孵。温孵样品采用甲醇沉淀蛋白处理后进行HPLC法分析，色谱条件：色谱柱为Diamonsil C₁₈柱；流动相为0.2%乙酸水溶液与甲醇，梯度洗脱；检测波长为260 nm；柱温为25℃；进样量为10 μl。结果大豆苷及其代谢物大豆苷元分别在98.0~490、57.6~288 μg/ml浓度范围内线性良好，高、中、低3个浓度的相对回收率在96.6%~114.0%之间，相对标准偏差小于9.9%，表明本法的准确度和精密度符合要求。大豆苷在肠道菌液中代谢生成大豆苷元，其消除半衰期为37.6 min。结论所建立的HPLC法适合同时测定体外大鼠肠道菌液中的大豆苷及其代谢物，大豆苷在体外代谢的主要途径为糖苷键断裂转化成对应苷元。
- 肠道菌群 /
- 体外代谢 /
- 大豆苷 /
- 高效液相色谱法
Abstract: Objective To establish an HPLC method for simultaneously determination of daidzin and its metabolite in rat intestinal bacteria test solution in vitro, and study the metabolism of daidzin by rat intestinal bacteria. Methods The incubation of daidzin was carried out at 37℃ with rat intestinal bacteria test solution. The samples were precipitated by methanol followed by a separation with HPLC method. The conditions were as follows:the chromatographic column was Ultimate C₁₈; the mobile phase was 0.2% acetic acid and methanol (gradient elution); the detection wavelength was 260nm, and the column temperature was 25℃. Results Daidzin and daidzein showed good linearity with the range of 98.0~490μg/ml and 57.6~288μg/ml, respectively. The accuracy of daidzin and daidzein was ranged from 96.6%~114.0% and the precision of the two analytes was within 9.9%. Daidzin was transformed to daidzein in the rat intestinal bacteria test solution, and its elimination half-life was 37.6 min. Conclusion The established HPLC method was suitable for the determination of daidzin and its metabolite. Daidzin could be metabolized by rat intestinal bacteria in vitro,and the main metabolic pathway was transforming daidzin into corresponding aglycone through breaking the glucoside bond
- intestinal bacteria /
- metabolism in vitro /
- daidzin /
- HPLC

[1]	刘志平,崔建国. 近三年环烯醚萜类新化合物的研究进展[J]. 天然产物研究与开发,2010,22(1):167-170.
[2]	王守英,李志春,卜勇军. 大豆异黄酮的生物学作用研究进展[J]. 新乡医学院学报,2005,22(4):397-400.
[3]	SILBERBERG M,MORAND C,MATHEVON T,et al. The bioavailability of polyphenols is highly governed by the capacity of the intestine and of the liver to secrete conjugated metabolites[J]. Eur J Nutr,2006,45(2):88-96.
[4]	张李赢,杨轶舜,张彤,等. 肠道菌群对中药苷类成分的代谢研究进展[J]. 中药材,2011,34(7):1155-1158.
[5]	VITALE DC,PIAZZA C,MELILLI B,et al. Isoflavones:estrogenic activity,biological effect and bioavailability[J]. Eur J Drug Metab Ph,2013,38(1):15-25.
[6]	SETCHELL KD,BROWN NM,ZIMMER-NECHEMIAS L,et al. Evidence for lack of absorption of soy isoflavone glycosides in humans,supporting the crucial role of intestinal metabolism for bioavailability[J]. Am J Clin Nutr,2002,76(2):447-453.
[7]	PISKULA MK,YAMAKOSHI J,IWAI Y. Daidzein and genistein but not their glucosides are absorbed from the rat stomach[J]. Febs Lett,1999,447(2-3):287-291.
[8]	CHANDRASEKHARAN S,AGLIN A. Pharmacokinetics of dietary isoflavones[J]. J Steroids Hormon Sci 2013,12(1):4.

[1]	江骏, 陈利, 李众, 刘金阁, 齐天, 周婷婷. 中药成分调控肠道菌群影响肠吸收转运的研究进展 . 药学实践与服务, 2024, 42(3): 89-93, 120. doi: 10.12206/j.issn.2097-2024.202303012
[2]	徐飞, 陈瑾, 鲁育含, 李志勇. 肠道菌群参与糖尿病肾病的机制研究进展 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202312023
[3]	陈思敏, 凌期盛, 张赛龙, 缪朝玉. 肠上皮Metrnl基因敲除对肠道菌群以及溃疡性结肠炎的影响 . 药学实践与服务, 2023, 41(11): 654-661. doi: 10.12206/j.issn.2097-2024.202211050
[4]	江玥曈, 高葱葱, 张玉佳, 欧炀育, 周婷婷. 黑豆、半成品和成品淡豆豉中6种异黄酮含量变化比较 . 药学实践与服务, 2023, 41(6): 372-376. doi: 10.12206/j.issn.2097-2024.202209085
[5]	郭羽晨, 丁华敏, 邓珊珊, 章越凡, 李铁军. 虎杖清脉饮对慢性脑缺血小鼠认知功能及肠道菌群的影响 . 药学实践与服务, 2023, 41(5): 302-309. doi: 10.12206/j.issn.2097-2024.202208106
[6]	范围晴, 夏天爽, 蒋益萍, 年华, 辛海量. 肠道菌群调控骨代谢及中药应用研究进展 . 药学实践与服务, 2023, 41(8): 459-464, 471. doi: 10.12206/j.issn.2097-2024.202204059
[7]	王倩男, 黄心慧, 杨民绪, 杨星瑞, 朱可涵, 周婷婷. 肠道菌群相关的抗抑郁治疗研究进展 . 药学实践与服务, 2022, 40(5): 422-426. doi: 10.12206/j.issn.2097-2024.202111034
[8]	郭良君, 孙亮, 郑巍, 陆萍, 傅明强, 郭建斌. 高效液相色谱法测定鼻炎灵胶囊中黄芩苷含量 . 药学实践与服务, 2017, 35(1): 64-66. doi: 10.3969/j.issn.1006-0111.2017.01.015
[9]	姜述文, 刘洁萍, 肖秋生, 肖金宝, 史春山, 孙丹, 姜宗文. 高效液相色谱法测定康得灵胶囊中黄芩苷的含量 . 药学实践与服务, 2016, 34(5): 447-449. doi: 10.3969/j.issn.1006-0111.2016.05.017
[10]	王婵, 韦刃, 黄晓玉, 林广, 黄志权, 周永强. 用高效液相色谱法测定银翘散配方颗粒中连翘酯苷A的含量 . 药学实践与服务, 2015, 33(2): 156-158. doi: 10.3969/j.issn.1006-0111.2015.02.017
[11]	王漪璇, 毋丹, 曹军宁, 钱隽. 人血浆中甲氨蝶呤的高效液相色谱法测定及其代谢物的质谱定性分析 . 药学实践与服务, 2015, 33(2): 143-146,158. doi: 10.3969/j.issn.1006-0111.2015.02.013
[12]	刘彬果, 张新萍. 高效液相色谱法测定气管炎颗粒中黄芩苷的含量 . 药学实践与服务, 2015, 33(5): 451-452. doi: 10.3969/j.issn.1006-0111.2015.05.019
[13]	张丽萍. 用高效液相色谱法测定安坤颗粒中栀子苷和芍药苷的含量 . 药学实践与服务, 2014, 32(2): 121-123. doi: 10.3969/j.issn.1006-0111.2014.02.012
[14]	纪松岗, 杨宇, 李康, 吴海棠, 朱臻宇. 柱前衍生化-高效液相色谱法测定白念珠菌中多胺的含量 . 药学实践与服务, 2013, 31(6): 412-414,453. doi: 10.3969/j.issn.1006-0111.2013.06.04
[15]	马建丽, 刘皈阳, 李翔, 黄欣欣, 姚飞, 马晓晖. 高效液相色谱法同时测定扁咽口服液中黄芩苷和肉桂酸的含量 . 药学实践与服务, 2013, 31(6): 451-453. doi: 10.3969/j.issn.1006-0111.2013.06.015
[16]	吴文飞, 张利斌, 李玉平. 高效液相色谱法测定支扩养阴颗粒中蒙花苷的含量 . 药学实践与服务, 2012, 30(4): 307-309.
[17]	谭生建, 刘刚, 张捷, 刘昌叶, 潘敏翔, 常华. 高效液相色谱法同时测定银黄口服液中绿原酸和黄芩苷含量 . 药学实践与服务, 2008, (2): 138-140.
[18]	叶光明, 瞿发林, 刘桂永, 谈丽娜, 蒋勤, 赵勇. 高效液相色谱法测定百年康口服液中橙皮苷的含量 . 药学实践与服务, 2006, (5): 293-295.
[19]	王建核. 高效液相色谱法测定脑脉通口服液中的黄芪甲苷 . 药学实践与服务, 2005, (6): 364-365.
[20]	李萍, 杨洁. 高效液相色谱法测定乙肝宁胶囊中芍药苷的含量 . 药学实践与服务, 2004, (4): 226-228.

点击查看大图

计量

文章访问数: 2018
HTML全文浏览量: 192
PDF下载量: 425
被引次数: 0

高效液相色谱法测定体外大鼠肠道菌液中大豆苷及其代谢物

doi: 10.3969/j.issn.1006-0111.2018.04.013

1. 第二军医大学药学院, 上海市药物(中药)代谢产物研究重点实验室, 上海 200433

2. 第二军医大学药学院, 上海市药物(中药)代谢产物研究重点实验室, 上海 200433;安徽中医药大学药学院, 安徽合肥 230012

基金项目: 国家自然科学基金资助项目（81573584，81773862）；第二军医大学本科生创新孵化基地项目（FM2016071）

收稿日期: 2017-10-02
修回日期: 2018-03-26

关键词:

肠道菌群 /

体外代谢 /

大豆苷 /

高效液相色谱法

摘要: 目的建立HPLC法测定体外大鼠肠道菌液中的大豆苷及其代谢物，研究大鼠肠道菌群对大豆苷的代谢情况。方法采用大鼠离体粪便温孵法，将大豆苷与大鼠肠道菌群培养液进行厌氧温孵。温孵样品采用甲醇沉淀蛋白处理后进行HPLC法分析，色谱条件：色谱柱为Diamonsil C₁₈柱；流动相为0.2%乙酸水溶液与甲醇，梯度洗脱；检测波长为260 nm；柱温为25℃；进样量为10 μl。结果大豆苷及其代谢物大豆苷元分别在98.0~490、57.6~288 μg/ml浓度范围内线性良好，高、中、低3个浓度的相对回收率在96.6%~114.0%之间，相对标准偏差小于9.9%，表明本法的准确度和精密度符合要求。大豆苷在肠道菌液中代谢生成大豆苷元，其消除半衰期为37.6 min。结论所建立的HPLC法适合同时测定体外大鼠肠道菌液中的大豆苷及其代谢物，大豆苷在体外代谢的主要途径为糖苷键断裂转化成对应苷元。

English Abstract

Simultaneously determination of daidzin and its metabolite in rat intestinal bacteria test solution in vitro by HPLC

1. School of Pharmacy & Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Second Military Medical University Shanghai 200433, China

2. School of Pharmacy & Shanghai Key Laboratory for Pharmaceutical Metabolite Research, Second Military Medical University Shanghai 200433, China;School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China

Received Date: 2017-10-02
Rev Recd Date: 2018-03-26

Keywords:

Abstract: Objective To establish an HPLC method for simultaneously determination of daidzin and its metabolite in rat intestinal bacteria test solution in vitro, and study the metabolism of daidzin by rat intestinal bacteria. Methods The incubation of daidzin was carried out at 37℃ with rat intestinal bacteria test solution. The samples were precipitated by methanol followed by a separation with HPLC method. The conditions were as follows:the chromatographic column was Ultimate C₁₈; the mobile phase was 0.2% acetic acid and methanol (gradient elution); the detection wavelength was 260nm, and the column temperature was 25℃. Results Daidzin and daidzein showed good linearity with the range of 98.0~490μg/ml and 57.6~288μg/ml, respectively. The accuracy of daidzin and daidzein was ranged from 96.6%~114.0% and the precision of the two analytes was within 9.9%. Daidzin was transformed to daidzein in the rat intestinal bacteria test solution, and its elimination half-life was 37.6 min. Conclusion The established HPLC method was suitable for the determination of daidzin and its metabolite. Daidzin could be metabolized by rat intestinal bacteria in vitro,and the main metabolic pathway was transforming daidzin into corresponding aglycone through breaking the glucoside bond

引用本文:

Citation:

全文HTML

参考文献 (8)

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

电话: （021）81871324,81871397 E-mail: yxsjzzs@163.com

网站备案号沪ICP备05003363号-1

本系统由北京仁和汇智信息技术有限公司开发

留言板

高效液相色谱法测定体外大鼠肠道菌液中大豆苷及其代谢物

doi: 10.3969/j.issn.1006-0111.2018.04.013

Simultaneously determination of daidzin and its metabolite in rat intestinal bacteria test solution in vitro by HPLC

计量

出版历程

高效液相色谱法测定体外大鼠肠道菌液中大豆苷及其代谢物

doi: 10.3969/j.issn.1006-0111.2018.04.013

1. 第二军医大学药学院, 上海市药物(中药)代谢产物研究重点实验室, 上海 200433 2. 第二军医大学药学院, 上海市药物(中药)代谢产物研究重点实验室, 上海 200433;安徽中医药大学药学院, 安徽 合肥 230012

English Abstract

Simultaneously determination of daidzin and its metabolite in rat intestinal bacteria test solution in vitro by HPLC

全文HTML

目录

1. 第二军医大学药学院, 上海市药物(中药)代谢产物研究重点实验室, 上海 200433

2. 第二军医大学药学院, 上海市药物(中药)代谢产物研究重点实验室, 上海 200433;安徽中医药大学药学院, 安徽合肥 230012