Study on hydrophilic chemical constituents from Thesium Chinensis Turcz
-
摘要: 目的 对百蕊草中亲水性化学成分进行研究。 方法 采用硅胶柱色谱、SephadexLH-20凝胶柱色谱等分离手段，对百蕊草水提取物经AB-8大孔树脂吸附，50%乙醇洗脱的亲水性组分（TT50）进行分离纯化，应用核磁共振波谱分析、结合文献报道鉴定化合物结构。 结果 从TT50中分离纯化得到6个化合物，分别鉴定为：山奈酚（ 1 ），紫云英苷（ 2 ），山奈酚-3，7-二-O-β-D-吡喃葡萄糖苷（ 3 ），山奈酚-3-O-L-吡喃鼠李糖基（1→2）-β-D-吡喃葡萄糖苷（ 4 ），山奈酚-3-O-L-吡喃鼠李糖基（1→2）-［6-O-乙酰基］-β-D-吡喃葡萄糖苷（ 5 ），芸香苷（ 6 ）。 结论 TT50中主要成分为以山奈酚为母核的黄酮苷类化合物，化合物 4 、 5 为首次从百蕊草中分离得到。Abstract: Objective To investigate the hydrophilic chemical constituents from Thesium Chinensis Turcz. Methods The water extract of Thesium Chinensis Turcz. was adsorbed onto AB-8 resin column and eluted with 50% ethanol to give the hydrophilic portion TT50. TT50 was further separated and purified by repeated column chromatography on silica gel,Sephadex LH-20. The structures of these purified compounds were identified by NMR spectral analysis and comparison with the reported data. Results Six compounds were isolated and identified as kaempferol ( 1 ),kaempferol-3-O-glucoside ( 2 ),kaepmferol-3,7-di-O-β-D-glucopyranoside ( 3 ),kaempferol-3-O-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside ( 4 ),kaemperol-3-O-α-L-rhamnopyranosyl(1→2)-[6-O-acetyl]-β-D-glucopyranoside ( 5 ),rutinoside ( 6 ). Conclusion The main constituents of TT50 were kaempferol glycosides. Compounds 4 and 5 were isolated from this plant for the first time.
-
Key words:
- Thesium chinensis Turcz /
- chemical constituents /
- flavonoid glycosides
-
[1] Parveen Z,Deng Y,Saeed MK,et al. Antiinflammatory and analgesic activities of Thesium chinense Turcz extracts and its major flavonoids,kaempferol and kaempferol-3-O-glucoside[J]. Yakugaku Zasshi,2007,127(8):1275-1279. [2] 宣伟东,唐大海,卞俊,等. 百蕊草对阿霉素肾病大鼠治疗作用的实验研究[J].药学实践杂志,2012,30(6):443-446. [3] 闫俊,唐大海,宣伟东,等. 百蕊草提取物对免疫球蛋白A肾病模型大鼠的治疗作用[J].药学实践杂志,2015,33(1):28-31. [4] Lee IK,Kim KH,Choi SU,et al. Photochemical constituents of Thesium Chinense Turcz and their cytotoxic activities in vitro[J]. Nat Prod Sci,2009,15(4):246-249. [5] Wu H,Dushenkov S,Ho CT. et al. Novel acetylated flavonoid glycosides from the leaves of Allium ursinum [J]. Food Chem,2009,115(2):592-595. [6] Norbaek R,Kondo T. Flavonol glycosides from flowers of Crocus speciosus and C. antalyensis[J]. Photochemistry,1999,51(8):1113-1119. [7] 邹懿,洪敏,杨笑芳. 百蕊草化学成分分离[J].中国实验方剂学杂志,2016,22(7):74-77 [8] 鲁云霞,汪俊松. 百蕊草的化学成分研究[J].中草药,2004,35(5):491-493. [9] 刘洋,邓玉林,张经华,等.百蕊草黄酮类化合物研究[J].中药材,2009,32(4):518-520. [10] 安徽省医学科学研究所植化室百蕊草组.百蕊草有效成分的化学研究(第一报)[J].中草药通讯,1976,8:6-12. -
点击查看大图
计量
- 文章访问数: 2364
- HTML全文浏览量: 347
- PDF下载量: 388
- 被引次数: 0
下载:
