Anti-hypoxia activity and its protective effects of Lishukang capsule on rat brain tissue at simulated high altitude hypoxia
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摘要: 目的 研究利舒康胶囊的抗缺氧活性及其对模拟高原缺氧大鼠脑组织的保护作用。 方法 采用小鼠常压密闭缺氧耐受力实验和小鼠急性减压缺氧耐受力实验联合评价利舒康胶囊的抗缺氧活性；采用低压氧舱模拟海拔8 000 m高原环境，观察缺氧前后大鼠脑组织病理显微结构的改变，并测定其中能量代谢和抗氧化应激相关指标。 结果 利舒康胶囊低、中、高剂量组均能有效延长常压密闭缺氧小鼠的存活时间（P<0.01），且存在剂量依赖性，其中，中、高剂量组明显优于红景天胶囊组（P<0.05或P<0.01）；利舒康胶囊低、中、高剂量组能够降低急性减压缺氧小鼠的死亡率（P<0.01），且具有剂量依赖性，其中，中、高剂量组的死亡率明显低于红景天胶囊组（P<0.01）；低压氧舱模拟高原减压缺氧实验结果显示，与正常对照组相比，减压缺氧后大鼠脑组织出现病理性损伤，脑组织中丙二醛（malondialdehyde，MDA）、过氧化氢（hydrogen peroxide，H2O2）、一氧化氮（nitric oxide，NO）、乳酸（lactic acid，LD）含量和乳酸脱氢酶（lactic dehydrogenase，LDH）活性显著升高（P<0.05或P<0.01），超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）、谷胱甘肽过氧化物酶（glutathion peroxidase，GPX）活性均显著降低（P<0.05或P<0.01），而经利舒康胶囊预处理后，大鼠脑组织的病理损伤有所缓解，脑组织中MDA、NO含量均显著下降（P<0.05或P<0.01），中、高剂量组H2O2、LD含量及LDH活性显著下降（P<0.05或P<0.01），高剂量组SOD、CAT、GPX活性均显著升高（P<0.05）。 结论 利舒康胶囊具有良好的抗高原缺氧活性，能够对模拟高原缺氧损伤大鼠起到保护作用，其机制可能与提高机体抗氧化能力，降低自由基损伤，缓解缺氧所致能量缺乏和代谢障碍有关。Abstract: Objective To investigate anti-hypoxia activity and protective effects of Lishukang capsule on rat brain tissue at simulated high altitude hypoxia. Methods The anti-hypoxic activity of Lishukang capsule was evaluated with normobaric hypoxia test and acute hypobaric hypoxia test in mice. In addition, rats were exposed to large hypobaric hypoxia chamber stimulating 8 000 m altitude. The pathological changes of rat brain tissue before and after hypoxia were observed. The oxidative stress indicators and metabolism parameters in brain were measured. Results The low, medium and high Lishukang doses can effectively prolong the survival time of mice (P<0.01) in the dose dependent manner. The medium and high Lishukang doses were significantly better than those of Rhodiola rosea capsules (P<0.05 or P<0.01). The low, medium, high Lishukang dose groups reduced the mortality of acute hypobaric hypoxia mice (P<0.01) with dose dependent effects. The mice mortality in medium and high dose groups was lower than that of Rhodiola rosea group (P<0.01). Compared with normal control group, the hypoxic model rats exhibited pathological injury in the brain tissue after exposure to hypobaric hypoxia stimulating 8 000 m altitude. The contents of MDA, H2O2, NO, LD and LDH activity increased significantly (P<0.05 or P<0.01), while the activities of SOD, CAT, GPX were significantly decreased (P<0.05 or P<0.01). After pretreatment with Lishukang capsule, the pathological damage of rat brain tissue was alleviated and the content of MDA, NO in the brain tissue was reduced (P<0.05 or P<0.01). The levels of H2O2, LD content and LDH activity in medium and high dose groups were significantly decreased (P<0.05 or P<0.01). The activities of SOD, CAT and GPX in high dose group were significantly increased (P<0.05). Conclusion Lishukang capsule has good anti-hypoxia activity. It provides protective effect for the injuries induced by hypobaric hypoxia in rats. The mechanism may related to the improvement of antioxidant capability, reduction of free radical damage and amelioration of energy metabolism.
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Key words:
- Lishukang capsule /
- high altitude hypoxia /
- brain tissue /
- protective effect
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