NVP-BEZ235对低氧大鼠肺血管管周肥大细胞的影响

柴晓宇; 韩倩; 吴艳萍; 王翔; 刘新民

doi:10.3969/j.issn.1006-0111.2017.05.005

NVP-BEZ235对低氧大鼠肺血管管周肥大细胞的影响

doi: 10.3969/j.issn.1006-0111.2017.05.005

北京大学第一医院老年科, 北京 100034

基金项目: 国家自然科学基金（81270114）

Effects of NVP-BEZ235 on pulmonary perivascular mast cells in hypoxia rats

Department of Geriatrics, First Hospital of Peking University, Beijing 100034, China

摘要: 目的评价PI3K/mTOR双重抑制剂NVP-BEZ235对低压、低氧诱导的大鼠肺血管管周肥大细胞的分布以及脱颗粒状态的影响。方法低氧组SD雄性大鼠于低压低氧培养箱（50.5 kPa）内培养，治疗组隔天给予NVP-BEZ235（35 mg/kg）干预，对照组匹配饲养条件并在当地气压常氧环境饲养，21 d后全部动物以5%戊巴比妥钠麻醉后取材。肺组织固定后进行石蜡包埋并行苏木素伊红染色和甲苯胺蓝染色。结果低氧组大鼠肺血管管周肥大细胞数量较对照组增多，聚集明显。低氧组不同管径的肺血管（<50 μm，50~100 μm，>100 μm）管周的肥大细胞聚集情况及脱颗粒细胞比例较对照组增多，具有统计学差异（P<0.05），而NVP-BEZ235干预组较低氧组肺血管管周肥大细胞数量减少（P<0.05），且能降低管径50~100 μm的肺血管周围低氧组脱颗粒的肥大细胞比例（P=0.000 3）。结论 NVP-BEZ235可以抑制低压低氧诱导的大鼠肺血管管周肥大细胞的聚集及中等管径血管管周脱颗粒细胞比例。
- 磷脂酰肌醇-3-激酶 /
- 肥大细胞 /
- 低氧 /
- 肺血管
Abstract: Objective To assess the effects of PI3K/mTOR dual inhibitor NVP-BEZ235 on the distribution and degranulation of perivascularmast cells in the lung of hypobaric hypoxia rats. Methods SD male rats in the hypoxia group were raised in the specific hypoxic incubator (50.5 kPa).The intervene group received NVP-BEZ235 training (35 mg/kg) intervention once every two days.The control group was fed in the conditions comparable to the other groups at local pressure and normoxia environment. After 21 days, all rats were sacrificed with injection of 5% pentobarbital sodium. Lung tissues were fixed and embedded in paraffin for further hematoxylin-eosin staining and toluidine blue staining. Results Compared with the control group, the number of pulmonary perivascular mast cells in the hypoxia group increased significantly. Hypoxia group had more mast cell accumulation and larger degranulation ratio around different diameters of pulmonary vessels (less than 50 μm, 50-100 μm, larger than 100 μm) than the control group (P< 0.05).The mast cell numbers decreased in NVP-BEZ235 intervention group compared with hypoxia group (P<0.05).The degranulation ratio of pulmonary vascular mast cells around 50-100 μm in the intervention group was smaller than the hypoxia group (P=0.000 3). Conclusion NVP-BEZ235 inhibits the aggregation of pulmonary perivascular mast cells and degranulation ratio of median diameter pulmonary perivascular mast cells in hypobaric hypoxia rats.
- phosphatidylionsitol-3-kinases /
- mast cell /
- hypoxia /
- pulmonary vascular

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NVP-BEZ235对低氧大鼠肺血管管周肥大细胞的影响

doi: 10.3969/j.issn.1006-0111.2017.05.005

Effects of NVP-BEZ235 on pulmonary perivascular mast cells in hypoxia rats

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NVP-BEZ235对低氧大鼠肺血管管周肥大细胞的影响

doi: 10.3969/j.issn.1006-0111.2017.05.005

北京大学第一医院老年科, 北京 100034

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Effects of NVP-BEZ235 on pulmonary perivascular mast cells in hypoxia rats

Department of Geriatrics, First Hospital of Peking University, Beijing 100034, China

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NVP-BEZ235对低氧大鼠肺血管管周肥大细胞的影响

doi: 10.3969/j.issn.1006-0111.2017.05.005

Effects of NVP-BEZ235 on pulmonary perivascular mast cells in hypoxia rats

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NVP-BEZ235对低氧大鼠肺血管管周肥大细胞的影响

doi: 10.3969/j.issn.1006-0111.2017.05.005

北京大学第一医院老年科, 北京 100034

English Abstract

Effects of NVP-BEZ235 on pulmonary perivascular mast cells in hypoxia rats

Department of Geriatrics, First Hospital of Peking University, Beijing 100034, China

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