Determination of baicalin in Biyanling capsule by HPLC
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摘要: 目的 建立高效液相色谱法测定鼻炎灵胶囊中黄芩苷的含量。 方法 色谱柱为Waters XBridge C18柱（4.6 mm×250 mm，5 μm），流动相为乙腈-0.8%甲酸（25：75），检测波长276 nm，流速1.0 ml/min，柱温30℃，进样量10 μl。 结果 黄芩苷在1.25~40 μg/ml范围内（r=0.999 9）呈良好的线性关系，精密度实验中RSD均小于2%，加样回收率在95%~100%之间。 结论 该测定方法简便、准确，分离效果好，可用于鼻炎灵胶囊的质量控制。Abstract: Objective To develop a HPLC method for determination of baicalin. Methods The separation was carried out on a Waters XBridge C18 column(4.6 mm×250 mm, 5 μm), the mobile phase was composed of acetonitrile and 0.8% formic acid (25:75), the detection wavelength was set at 276 nm, the flow rate was 1.0 ml/min, the column temperature was 30℃ and the injection volume was 10 μl. Results The linearity was obtained over 1.25-40 μg/ml(r=0.999 9) for baicalin. The RSD of precision were less than 2%. The average recovery was between 95% and 100%. Conclusion This HPLC method was simple, accuracy and suitable for the quality control of Biyanling capsule.
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Key words:
- HPLC /
- Biyanling capsule /
- baicalin /
- content determination
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