白子草总黄酮及总多糖降血糖有效部位的提取工艺研究

曾令军; 柯小温; 宋洪涛

doi:10.3969/j.issn.1006-0111.2016.06.009

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白子草总黄酮及总多糖降血糖有效部位的提取工艺研究

曾令军, 柯小温, 宋洪涛

文章导航 > 药学实践与服务 > 2016 > 34(6): 516-521

曾令军, 柯小温, 宋洪涛. 白子草总黄酮及总多糖降血糖有效部位的提取工艺研究[J]. 药学实践与服务, 2016, 34(6): 516-521. doi: 10.3969/j.issn.1006-0111.2016.06.009

引用本文:

曾令军, 柯小温, 宋洪涛. 白子草总黄酮及总多糖降血糖有效部位的提取工艺研究[J]. 药学实践与服务, 2016, 34(6): 516-521. doi: 10.3969/j.issn.1006-0111.2016.06.009

ZENG Lingjun, KE Xiaowen, SONG Hongtao. Study on extraction technology of blood glucose-lowering components of total flavonoids and polysaccharides from Gynura divaricata (L.) DC.[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(6): 516-521. doi: 10.3969/j.issn.1006-0111.2016.06.009

Citation:

ZENG Lingjun, KE Xiaowen, SONG Hongtao. Study on extraction technology of blood glucose-lowering components of total flavonoids and polysaccharides from Gynura divaricata (L.) DC.[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(6): 516-521. doi: 10.3969/j.issn.1006-0111.2016.06.009

白子草总黄酮及总多糖降血糖有效部位的提取工艺研究

doi: 10.3969/j.issn.1006-0111.2016.06.009

原南京军区福州总医院药学科, 福建福州 350025

基金项目: 福建省自然科学基金（2012J01400）

Study on extraction technology of blood glucose-lowering components of total flavonoids and polysaccharides from Gynura divaricata (L.) DC.

Department of Pharmacy, Fuzhou General Hospital of Nanjing Military Region, Fuzhou 350025, China

摘要: 目的研究建立白子草总黄酮及总多糖降血糖有效部位的提取工艺。方法在单因素试验的基础上，考察提取次数、药材粒径、浸泡时间、料/液比、乙醇浓度、提取时间对白子草总黄酮提取率的影响，并通过L₉（3）⁴正交试验优化总黄酮最佳提取工艺；以前期研究为基础，结合L₉（3）⁴正交试验，优化总多糖最佳提取工艺。结果白子草总黄酮最佳提取工艺为：药材最粗粉加20倍80%乙醇，浸泡0.5 h，回流提取2次，每次1.0 h；总多糖最佳提取工艺为：醇提后药渣加20倍纯净水，80℃回流提取2次，每次1.0 h。该条件下白子草总黄酮及总多糖提取率均大于80%。结论本研究建立的白子草总黄酮及总多糖降血糖有效部位提取工艺稳定、重复性好，为白子草降血糖活性研究奠定了实验基础。
- 白子草 /
- 总黄酮 /
- 总多糖 /
- 提取工艺
Abstract: Objective To study and establish the extraction technology of hypoglycemic components of total flavonoids and polysaccharides from Gynura divaricata (L.) DC.. Methods Based on the singer factor test, the influences of extracting times, diameter of drug powder, soaking time, solid-liquid ratio, ethanol concentration, and extracting time on extraction ratio of total flavonoids were studied. The best extraction technology of total flavonoids from Gynura divaricata (L.) DC. was established through L₉(3)⁴ orthogonal tests. On the base of previous studies combine with L₉(3)⁴ orthogonal tests, the best extraction technology of total polysaccharides from Gynura divaricata (L.) DC. was established. Results The optimized extraction technology of total flavonoids was confirmed as follows:drug powder of less than 10 mesh diameter were soaking 0.5 hour with 80% ethanol, and solid-liquid ratio 1:20, and extracting twice, each time one hour. The optimized extraction technology of total polysaccharides was confirmed as follows:add 20 times water to the residue of ethanol, extraction temperature 90℃, and extracting twice, each time one hour. The extraction rate of both total flavonoids and polysaccharides were more than 80%. Conclusion The extraction technology of hypoglycemic components of total flavonoids and polysaccharides from Gynura divaricata (L.) DC. established in this study is stable, reproducible, and lays a foundation for the further studies on hypoglycemic components in Gynura divaricata (L.) DC.
- Gynura divaricata (L.) DC. /
- total flavonoids /
- total polysaccharides /
- extraction technology

[1]	黄有霖. 福建省中药材标准[S]. 福建:海风出版社,2006:70-73.
[2]	胡勇,李维林,林厚文,等. 白背三七地上部分降血糖作用研究[J]. 西南林学院学报,2007,27(1):54-58.
[3]	姜曼花,胡剑卓,邱文高,等. 白背三七多糖和黄酮降血糖及耐缺氧作用[J]. 中国医院药学杂志,2009,29(13):1074-1076.
[4]	马正东,陈磊,宋洪涛,等. 白背三七水提取物对2型糖尿病大鼠的降血糖作用及其机制[J]. 中草药,2010,41(4):623-626.
[5]	俞浩,毛斌斌,周国梁,等. 白背三七总黄酮对糖尿病大鼠的降血糖作用[J]. 食品科学,2013,34(15):295-298.
[6]	张钱钱. 白子草多糖的分离纯化及其降血糖作用研究[D]. 福建:福建中医药大学,2014.
[7]	王津江,陈磊,宋洪涛,等. 白背三七总黄酮的提取工艺研究[J]. 中国药师,2009,12(2):146-149.

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[20]	周永刚, 卞艳芳, 陈万生. 知母总多糖的含量测定 . 药学实践与服务, 1999, (5): 306-307.

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白子草总黄酮及总多糖降血糖有效部位的提取工艺研究

doi: 10.3969/j.issn.1006-0111.2016.06.009

原南京军区福州总医院药学科, 福建福州 350025

基金项目: 福建省自然科学基金（2012J01400）

收稿日期: 2015-10-20
修回日期: 2016-04-28

关键词:

白子草 /

总黄酮 /

总多糖 /

提取工艺

摘要: 目的研究建立白子草总黄酮及总多糖降血糖有效部位的提取工艺。方法在单因素试验的基础上，考察提取次数、药材粒径、浸泡时间、料/液比、乙醇浓度、提取时间对白子草总黄酮提取率的影响，并通过L₉（3）⁴正交试验优化总黄酮最佳提取工艺；以前期研究为基础，结合L₉（3）⁴正交试验，优化总多糖最佳提取工艺。结果白子草总黄酮最佳提取工艺为：药材最粗粉加20倍80%乙醇，浸泡0.5 h，回流提取2次，每次1.0 h；总多糖最佳提取工艺为：醇提后药渣加20倍纯净水，80℃回流提取2次，每次1.0 h。该条件下白子草总黄酮及总多糖提取率均大于80%。结论本研究建立的白子草总黄酮及总多糖降血糖有效部位提取工艺稳定、重复性好，为白子草降血糖活性研究奠定了实验基础。

English Abstract

Study on extraction technology of blood glucose-lowering components of total flavonoids and polysaccharides from Gynura divaricata (L.) DC.

Department of Pharmacy, Fuzhou General Hospital of Nanjing Military Region, Fuzhou 350025, China

Received Date: 2015-10-20
Rev Recd Date: 2016-04-28

Keywords:

Abstract: Objective To study and establish the extraction technology of hypoglycemic components of total flavonoids and polysaccharides from Gynura divaricata (L.) DC.. Methods Based on the singer factor test, the influences of extracting times, diameter of drug powder, soaking time, solid-liquid ratio, ethanol concentration, and extracting time on extraction ratio of total flavonoids were studied. The best extraction technology of total flavonoids from Gynura divaricata (L.) DC. was established through L₉(3)⁴ orthogonal tests. On the base of previous studies combine with L₉(3)⁴ orthogonal tests, the best extraction technology of total polysaccharides from Gynura divaricata (L.) DC. was established. Results The optimized extraction technology of total flavonoids was confirmed as follows:drug powder of less than 10 mesh diameter were soaking 0.5 hour with 80% ethanol, and solid-liquid ratio 1:20, and extracting twice, each time one hour. The optimized extraction technology of total polysaccharides was confirmed as follows:add 20 times water to the residue of ethanol, extraction temperature 90℃, and extracting twice, each time one hour. The extraction rate of both total flavonoids and polysaccharides were more than 80%. Conclusion The extraction technology of hypoglycemic components of total flavonoids and polysaccharides from Gynura divaricata (L.) DC. established in this study is stable, reproducible, and lays a foundation for the further studies on hypoglycemic components in Gynura divaricata (L.) DC.

曾令军, 柯小温, 宋洪涛. 白子草总黄酮及总多糖降血糖有效部位的提取工艺研究[J]. 药学实践与服务, 2016, 34(6): 516-521. doi: 10.3969/j.issn.1006-0111.2016.06.009

引用本文:

曾令军, 柯小温, 宋洪涛. 白子草总黄酮及总多糖降血糖有效部位的提取工艺研究[J]. 药学实践与服务, 2016, 34(6): 516-521. doi: 10.3969/j.issn.1006-0111.2016.06.009

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白子草总黄酮及总多糖降血糖有效部位的提取工艺研究

doi: 10.3969/j.issn.1006-0111.2016.06.009

Study on extraction technology of blood glucose-lowering components of total flavonoids and polysaccharides from Gynura divaricata (L.) DC.

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白子草总黄酮及总多糖降血糖有效部位的提取工艺研究

doi: 10.3969/j.issn.1006-0111.2016.06.009

原南京军区福州总医院药学科, 福建 福州 350025

English Abstract

Study on extraction technology of blood glucose-lowering components of total flavonoids and polysaccharides from Gynura divaricata (L.) DC.

Department of Pharmacy, Fuzhou General Hospital of Nanjing Military Region, Fuzhou 350025, China

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原南京军区福州总医院药学科, 福建福州 350025