HPLC method to assay the content of psoralen and angelicin in Zhuanggu granules
-
摘要: 目的 建立高效液相色谱法测定壮骨颗粒中补骨脂素和异补骨脂素的含量。 方法 色谱柱为Agilent Zorbax C18柱（4.6 mm×250 mm，5 μm），流动相为甲醇-水（45：55），检测波长为245 nm，流速为1.0 ml/min，柱温为25 ℃，进样量为10 μl。采用70%乙醇水浴方法提取壮骨颗粒中的补骨脂素和异补骨脂素。 结果 补骨脂素与异补骨脂素在3.75~40 μg/ml范围内呈良好的线性关系，对方法精密度（n=6）、重复性（n=6）和稳定性（12 h）进行考察，相对标准偏差（RSD）均小于2%。加样回收率（n=6）为94%~105%。 结论 该测定方法简便、快速、准确，可用于壮骨颗粒的临床快速质量控制。Abstract: Objective To develop a HPLC method for psoralenand angelicin content assay in Zhuanggu granules. Methods Agilent Zorbax C18 column(4.6 mm×250 mm, 5 μm) was used with methanol and water(45:55) as mobile phase. The detection wavelength was set at 245 nm. The flow rate was 1.0 ml/min and column temperature at 25 ℃. The injection volume was 10 μl.Psoralenand angelicin were extracted with 70% ethanol under water bath. Results The linearity was obtained over 3.75~40 μg/mlfor psoralenand angelicin. The RSDs were less than 2%. The average recovery was between 94% and 105%. Conclusion This simple and accurate HPLC method was suitable for the quality control of Zhuanggu granules.
-
Key words:
- HPLC /
- Zhuanggu granules /
- psoralen /
- angelicin
-
[1] 翟远坤,潘亚磊,牛银波,等.补骨脂素与异补骨脂素对乳鼠颅骨成骨细胞分化成熟影响的比较研究[J].中国药理学通报,2012,28(3):355-361. [2] 武密山,赵素芝,任立中,等.补骨脂素对成骨细胞核因子-κB受体激活配体和骨保护素表达的影响[J].中国老年学杂志,2012,32(1):66-69. [3] 国家药典委员会.中华人民共和国药典2015年版一部[S].北京:中国医药科技出版社,2015:187. [4] 江 丽,申国庆,龚春燕.HPLC法同时测定复方紫灵胶囊中补骨脂素、异补骨脂素、大黄素和丹参酮ⅡA的含量[J].中国药师,2013,16(10):1482-1485. [5] 刘俊娥,刘俊红,赵铁彦.高效液相色谱法测定健脾益肾颗粒中补骨脂素和异补骨脂素的含量[J].中国医药导报,2011,8(34):70-72. -
点击查看大图
计量
- 文章访问数: 2738
- HTML全文浏览量: 270
- PDF下载量: 463
- 被引次数: 0
下载:
