养心氏片防治慢性缺血性心力衰竭及急性心肌缺血再灌注损伤的实验研究

高越; 陈安妮; 赵亚红; 薛丹; 安泳潼; 柴逸峰; 张海

doi:10.3969/j.issn.1006-0111.2016.05.006

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

养心氏片防治慢性缺血性心力衰竭及急性心肌缺血再灌注损伤的实验研究

高越, 陈安妮, 赵亚红, 薛丹, 安泳潼, 柴逸峰, 张海

文章导航 > 药学实践与服务 > 2016 > 34(5): 403-407,415

高越, 陈安妮, 赵亚红, 薛丹, 安泳潼, 柴逸峰, 张海. 养心氏片防治慢性缺血性心力衰竭及急性心肌缺血再灌注损伤的实验研究[J]. 药学实践与服务, 2016, 34(5): 403-407,415. doi: 10.3969/j.issn.1006-0111.2016.05.006

引用本文:

GAO Yue, CHEN Anni, ZHAO Yahong, XUE Dan, AN Yongtong, CHAI Yifeng, ZHANG Hai. Experimental study on the effects of Yangxinshi tablets in the prevention and treatment of chronic ischemic heart failure and acute myocardial ischemia reperfusion injury[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(5): 403-407,415. doi: 10.3969/j.issn.1006-0111.2016.05.006

Citation:

GAO Yue, CHEN Anni, ZHAO Yahong, XUE Dan, AN Yongtong, CHAI Yifeng, ZHANG Hai. Experimental study on the effects of Yangxinshi tablets in the prevention and treatment of chronic ischemic heart failure and acute myocardial ischemia reperfusion injury[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(5): 403-407,415. doi: 10.3969/j.issn.1006-0111.2016.05.006

养心氏片防治慢性缺血性心力衰竭及急性心肌缺血再灌注损伤的实验研究

doi: 10.3969/j.issn.1006-0111.2016.05.006

1.
第二军医大学药学院实验教学中心, 上海 200433
2.
第二军医大学东方肝胆外科医院药材科, 上海 200438
3.
上海医药集团股份有限公司中央研究院, 上海 200123

基金项目: 上海市科委产学研医结合项目（15DZ1900102）

Experimental study on the effects of Yangxinshi tablets in the prevention and treatment of chronic ischemic heart failure and acute myocardial ischemia reperfusion injury

1.
Center of Experimental Teaching, College of Pharmacy, Second Military Medical University, Shanghai 200433, China
2.
Department of Pharmacy, Shanghai Eastern Hepatobiliary Surgery Hospital of Second Military Medical University, Shanghai, 200438, China
3.
Central Research Institute, Shanghai Pharmaceuticals Holding Co.Ltd., Shanghai 200123, China

摘要: 目的对养心氏片防治慢性缺血性心力衰竭和急性心肌缺血再灌注损伤作用进行实验研究，为其临床应用提供理论依据。方法采用冠状动脉结扎法构建慢性缺血性心力衰竭大鼠模型及急性心肌缺血再灌注小鼠心梗模型，随机分为假手术组、模型组、阳性药组、以及养心氏片低、中、高剂量组，通过预给药和造模后给药，检测大鼠超声心电图、ACE、ACD及小鼠心肌梗死面积、CK、LDH、SOD、相关因子，并观察心脏病理学切片的形态学变化。结果与假手术组相比，模型组的生化指标差异具有统计学意义（P<0.001）；与模型组相比，阳性药组、高剂量养心氏片组生化指标差异具有统计学意义（P<0.001，P<0.01，P<0.05），且能显著减少心肌梗死面积（P<0.01，P<0.05），明显改善心肌细胞水肿程度，减少细胞浸润。结论养心氏片对慢性缺血性心力衰竭、急性心肌缺血再灌注损伤具有明显的预防和治疗作用。
- 养心氏片 /
- 慢性缺血性心力衰竭 /
- 急性心肌缺血再灌注
Abstract: Objective Experimental study on effects of Yangxinshi tablets in the prevention and treatment of chronic ischemic heart failure and acute myocardial ischemia reperfusion injury in order to provide a theoretical basis for its clinical application. Method Rat model of chronic ischemic heart failure and acute ischemia reperfusion mouse model of myocardial infarction were constructed through coronary artery ligation. Those animals were randomized to sham operation group, model group, positive drug group, high Yangxinshi tablet dose group, middle Yangxinshi tablet dose group, low Yangxinshi tablet dose group. Rat electrocardiogram, ultrasound, ACE, ACD, TNF-a, mouse myocardial infarction area, CK, LDH, SOD and related factors were recorded during pre-dosing and post-dosing after modeling. Morphological changes were observed with cardiac pathological section. Results The biochemical indicators in model group were significantly different from sham operation group with statistical significance (P<0.001). Compared to the model group, positive drug group and high Yangxinshi tablet dose group exhibited biochemical differences with statistical significance (P<0.001, P<0.01, P<0.05), significant myocardial infarction area reduction (P<0.01, P<0.05), improvement in myocardial cells and reduction in the cell infiltration. Conclusion Yangxinshi tablets have preventive and therapeutic effects on chronic ischemic heart failure and acute ischemia reperfusion injury.
- Yangxinshi tablet /
- chronic ischemic heart failure /
- acute myocardial ischemia reperfusion

[1]	姜作玲,高莉, 王辉,等.养心氏片对大鼠冠脉结扎致心肌缺血模型的影响[J].世界中医药,2015,10(4):553-556.
[2]	崔燕,张辉,庄婕.养心氏片治疗心绞痛及冠心病临床试验资料研究[J].食品与药品, 2013,15(4):293-295.
[3]	盘锋.养心氏片辅助治疗舒张性心力衰竭80例疗效观察[J].中医临床研究,2014,6(18):39-41.
[4]	瞿家武,虞江灏.养心氏片治疗慢性心力衰竭临床疗效观察[J].实用临床医药杂志,2010,14(15):89-97.
[5]	王靓,黄金玲,施慧,等.对慢性心力衰竭实验动物模型制作方法的研究进展[J].当代医药论丛,2014,12(10):291-293.
[6]	韩曼,马莉,于远望.2种慢性心力衰竭大鼠模型的建立[J].新乡医学院学报,2015,32(11):992-995.
[7]	刘建,范慧敏,汪进益,等.小鼠心梗模型的建立与无创评价[J].中国实验动物学报,2010,18(3):196-198.

[1]	竺东杰, 贺新征, 邹杰, 余史丹, 李红霞. 雷公藤甲素对大鼠脑缺血再灌注损伤的影响及机制研究 . 药学实践与服务, 2024, 42(): 1-6. doi: 10.12206/j.issn.2097-2024.202311021
[2]	杨世畅, 李雪梅, 王曙霞, 何荆贵. 盐酸美金刚治疗缺血性脑卒中研究进展 . 药学实践与服务, 2018, 36(4): 293-296. doi: 10.3969/j.issn.1006-0111.2018.04.002
[3]	李莉, 冯晶晶, 李铁军, 章越凡. 复元醒脑汤对大鼠局灶性脑缺血再灌注损伤的保护作用研究 . 药学实践与服务, 2018, 36(1): 34-39. doi: 10.3969/j.issn.1006-0111.2018.01.007
[4]	程晶晶, 刘谋治, 李洪娇, 阎澜, 姜远英, 颜天华. 新化合物TG6对心肌缺血/再灌注损伤的影响及机制研究 . 药学实践与服务, 2014, 32(6): 440-443. doi: 10.3969/j.issn.1006-0111.2014.06.010
[5]	唐庆年, 伍倩, 田淑娟, 毛峻琴. 黄杞叶和片姜黄提取物及其组方对大鼠急性心肌缺血的保护和协同作用 . 药学实践与服务, 2013, 31(2): 120-123. doi: 10.3969/j.issn.1006-0111.2013.02.010
[6]	倪敏, 沈甫明. 肝脏缺血再灌注损伤发生机制及药物干预研究进展 . 药学实践与服务, 2011, 29(3): 179-180,211.
[7]	陆文铨, 张国强, 陈大贵, 张川. 丹参素对大鼠急性心肌缺血的保护作用 . 药学实践与服务, 2010, 28(4): 279-282.
[8]	田谋利, 李铁军, 刘虎, 邹最, 石学银. 尿苷三磷酸对大鼠脑缺血再灌注损伤保护作用的机制初探 . 药学实践与服务, 2010, 28(3): 181-183,234.
[9]	许文雅, 郭炜, 顾晓慧, 杨新鹏, 章忱, 吕嵘, 卫洪昌. 益心口服液预处理对大鼠急性心肌缺血的保护作用 . 药学实践与服务, 2008, (6): 422-426.
[10]	于大海. 刺五加总皂苷对大鼠心肌缺血性再灌注的保护作用及机制研究 . 药学实践与服务, 2008, (3): 197-199,232.
[11]	李小宁, 卫新国, 尚云, 卫洪昌, 朱晓梅, 孙平龙, 严世芸. 黄芪保心汤对急性心肌缺血大鼠血浆ET、CGRP水平的影响 . 药学实践与服务, 2001, (5): 270-272.
[12]	胡佳乐, 张川, 苏定冯. G-CSF减轻大鼠下肢缺血再灌注致肺功能损伤的实验研究 . 药学实践与服务, 2001, (1): 14-16.
[13]	胡佳乐, 李伟, 沈甫明. 腺苷预适应对缺血再灌注损伤的保护作用 . 药学实践与服务, 2001, (4): 206-208,205.
[14]	卫洪昌, 王秀薇, 吕嵘, 程金波, 朱晓梅, 吴倩, 章忱. 益心口服液药物预处理对大鼠心肌缺血再灌注损伤的影响 . 药学实践与服务, 2001, (3): 157-159.
[15]	孙平龙, 朱晓梅, 卫洪昌. 徐长卿内关穴位注射对大鼠心肌缺血再灌注损伤的影响 . 药学实践与服务, 2000, (4): 212-215.
[16]	卫洪昌, 朱冬胜, 朱晓梅, 孙平龙, 戴薇薇. 益心口服液预处理对急性心肌缺血大鼠CEC、血浆ET、CGRP及心肌病理形态学的影响 . 药学实践与服务, 1999, (4): 202-204.
[17]	孙鹏. 急性缺血性卒中的药物治疗进展 . 药学实践与服务, 1998, (6): 325-328.
[18]	李飒, 李万亥, 陶学斌, 陈东明, 陈洁. 二乙基二硫代氨基甲酸钠对沙土鼠脑缺血再灌注损伤的保护作用 . 药学实践与服务, 1995, (3): 136-139.
[19]	何钦军, 魏水易. 尼莫地平治疗缺血性中风的临床研究 . 药学实践与服务, 1993, (2): 98-100.
[20]	陶学斌, 李万亥. 钙超载与心肌缺血再灌注损伤 . 药学实践与服务, 1993, (4): 242-244.

点击查看大图

计量

文章访问数: 3628
HTML全文浏览量: 322
PDF下载量: 514
被引次数: 0

养心氏片防治慢性缺血性心力衰竭及急性心肌缺血再灌注损伤的实验研究

doi: 10.3969/j.issn.1006-0111.2016.05.006

1. 第二军医大学药学院实验教学中心, 上海 200433

2. 第二军医大学东方肝胆外科医院药材科, 上海 200438

3. 上海医药集团股份有限公司中央研究院, 上海 200123

基金项目: 上海市科委产学研医结合项目（15DZ1900102）

收稿日期: 2016-07-20
修回日期: 2016-08-23

关键词:

养心氏片 /

慢性缺血性心力衰竭 /

急性心肌缺血再灌注

摘要: 目的对养心氏片防治慢性缺血性心力衰竭和急性心肌缺血再灌注损伤作用进行实验研究，为其临床应用提供理论依据。方法采用冠状动脉结扎法构建慢性缺血性心力衰竭大鼠模型及急性心肌缺血再灌注小鼠心梗模型，随机分为假手术组、模型组、阳性药组、以及养心氏片低、中、高剂量组，通过预给药和造模后给药，检测大鼠超声心电图、ACE、ACD及小鼠心肌梗死面积、CK、LDH、SOD、相关因子，并观察心脏病理学切片的形态学变化。结果与假手术组相比，模型组的生化指标差异具有统计学意义（P<0.001）；与模型组相比，阳性药组、高剂量养心氏片组生化指标差异具有统计学意义（P<0.001，P<0.01，P<0.05），且能显著减少心肌梗死面积（P<0.01，P<0.05），明显改善心肌细胞水肿程度，减少细胞浸润。结论养心氏片对慢性缺血性心力衰竭、急性心肌缺血再灌注损伤具有明显的预防和治疗作用。

English Abstract

Experimental study on the effects of Yangxinshi tablets in the prevention and treatment of chronic ischemic heart failure and acute myocardial ischemia reperfusion injury

1. Center of Experimental Teaching, College of Pharmacy, Second Military Medical University, Shanghai 200433, China

2. Department of Pharmacy, Shanghai Eastern Hepatobiliary Surgery Hospital of Second Military Medical University, Shanghai, 200438, China

3. Central Research Institute, Shanghai Pharmaceuticals Holding Co.Ltd., Shanghai 200123, China

Received Date: 2016-07-20
Rev Recd Date: 2016-08-23

Keywords:

Abstract: Objective Experimental study on effects of Yangxinshi tablets in the prevention and treatment of chronic ischemic heart failure and acute myocardial ischemia reperfusion injury in order to provide a theoretical basis for its clinical application. Method Rat model of chronic ischemic heart failure and acute ischemia reperfusion mouse model of myocardial infarction were constructed through coronary artery ligation. Those animals were randomized to sham operation group, model group, positive drug group, high Yangxinshi tablet dose group, middle Yangxinshi tablet dose group, low Yangxinshi tablet dose group. Rat electrocardiogram, ultrasound, ACE, ACD, TNF-a, mouse myocardial infarction area, CK, LDH, SOD and related factors were recorded during pre-dosing and post-dosing after modeling. Morphological changes were observed with cardiac pathological section. Results The biochemical indicators in model group were significantly different from sham operation group with statistical significance (P<0.001). Compared to the model group, positive drug group and high Yangxinshi tablet dose group exhibited biochemical differences with statistical significance (P<0.001, P<0.01, P<0.05), significant myocardial infarction area reduction (P<0.01, P<0.05), improvement in myocardial cells and reduction in the cell infiltration. Conclusion Yangxinshi tablets have preventive and therapeutic effects on chronic ischemic heart failure and acute ischemia reperfusion injury.

引用本文:

Citation:

GAO Yue, CHEN Anni, ZHAO Yahong, XUE Dan, AN Yongtong, CHAI Yifeng, ZHANG Hai. Experimental study on the effects of Yangxinshi tablets in the prevention and treatment of chronic ischemic heart failure and acute myocardial ischemia reperfusion injury[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(5): 403-407,415. doi: 10.3969/j.issn.1006-0111.2016.05.006

全文HTML

参考文献 (7)

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

电话: （021）81871324,81871397 E-mail: yxsjzzs@163.com

网站备案号沪ICP备05003363号-1

本系统由北京仁和汇智信息技术有限公司开发

留言板

养心氏片防治慢性缺血性心力衰竭及急性心肌缺血再灌注损伤的实验研究

doi: 10.3969/j.issn.1006-0111.2016.05.006

Experimental study on the effects of Yangxinshi tablets in the prevention and treatment of chronic ischemic heart failure and acute myocardial ischemia reperfusion injury

计量

出版历程

养心氏片防治慢性缺血性心力衰竭及急性心肌缺血再灌注损伤的实验研究

doi: 10.3969/j.issn.1006-0111.2016.05.006

1. 第二军医大学药学院实验教学中心, 上海 200433 2. 第二军医大学东方肝胆外科医院药材科, 上海 200438 3. 上海医药集团股份有限公司中央研究院, 上海 200123

English Abstract

Experimental study on the effects of Yangxinshi tablets in the prevention and treatment of chronic ischemic heart failure and acute myocardial ischemia reperfusion injury

全文HTML

目录

1. 第二军医大学药学院实验教学中心, 上海 200433

2. 第二军医大学东方肝胆外科医院药材科, 上海 200438

3. 上海医药集团股份有限公司中央研究院, 上海 200123