公石松水提液对小鼠运动能力与血清乳酸、尿素氮及肝糖原的影响

石鹤坤; 陈开杰; 林小凤; 陈锦珊

doi:10.3969/j.issn.1006-0111.2016.05.005

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公石松水提液对小鼠运动能力与血清乳酸、尿素氮及肝糖原的影响

石鹤坤, 陈开杰, 林小凤, 陈锦珊

文章导航 > 药学实践与服务 > 2016 > 34(5): 399-402

石鹤坤, 陈开杰, 林小凤, 陈锦珊. 公石松水提液对小鼠运动能力与血清乳酸、尿素氮及肝糖原的影响[J]. 药学实践与服务, 2016, 34(5): 399-402. doi: 10.3969/j.issn.1006-0111.2016.05.005

引用本文:

SHI Hekun, CHEN Kaijie, LIN Xiaofeng, CHEN Jinsan. Effects of Ludisia discolor aqueous extract on exercise capacity, serum lactic acid, urea nitrogen and hepatic glycogen in mice[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(5): 399-402. doi: 10.3969/j.issn.1006-0111.2016.05.005

Citation:

SHI Hekun, CHEN Kaijie, LIN Xiaofeng, CHEN Jinsan. Effects of Ludisia discolor aqueous extract on exercise capacity, serum lactic acid, urea nitrogen and hepatic glycogen in mice[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(5): 399-402. doi: 10.3969/j.issn.1006-0111.2016.05.005

公石松水提液对小鼠运动能力与血清乳酸、尿素氮及肝糖原的影响

doi: 10.3969/j.issn.1006-0111.2016.05.005

解放军175医院/厦门大学附属东南医院药学科, 福建漳州 363000

基金项目: 解放军175医院青年苗圃基金资助项目（13Y017）

Effects of Ludisia discolor aqueous extract on exercise capacity, serum lactic acid, urea nitrogen and hepatic glycogen in mice

Department of Pharmacy, No.175 Hospital of PLA/Affiliated Southeast Hospital of Xiamen University, Zhangzhou, 363000 China

摘要: 目的探讨公石松水提液对抗小鼠运动性疲劳的作用及其机制。方法雄性小鼠随机分为5组：公石松水提液低、中、高剂量组[分别用公石松1.22、2.44、4.88 g/（kg·d）]、阳性对照组[红景天水提物2.34 g/（kg·d）]和空白对照组（等量蒸馏水），连续灌胃7 d 后，测定小鼠负重游泳时间及游泳90 min后血乳酸、血尿素氮及肝糖原指标。结果公石松水提液各组均可显著降低小鼠血尿素氮水平（P<0.05），增加肝糖原含量（P<0.05），呈量效关系；公石松水提液高剂量组可降低血乳酸，延长小鼠负重游泳时间（P<0.05）。其作用与阳性对照红景天水提物相当。结论公石松水提液具有抗小鼠运动性疲劳的作用，其作用机制可能通过提高肝糖原储备、增加葡萄糖的有氧分解、减少葡萄糖的无氧酵解及减少蛋白质的分解有关。
- 公石松水提液 /
- 运动性疲劳 /
- 肝糖原 /
- 血乳酸 /
- 血尿素氮
Abstract: Objective To investigate the effect against exercise-indused fatigue in mice by aqueous extract of Ludisia discolor and its mechanism. Methods Male mice were randomly divided into five groups including low, middle, high dose groups [aqueous extract of Ludisia discolor at dose of 1.22, 2.44, 4.88 g/(kg·d) respectively], positive control group [aqueous extract of Rhodiola, at dose of 2.34 g/(kg·d)] and control group (distilled water). After intragastric administration for seven days, mice were measured for loading swimming time, and were tested 90 minutes after loading swimming for blood urea nitrogen, blood lactic acid and hepatic glycogen levels. Results The blood urea nitrogen was significantly decreased (P<0.05) in aqueous extract of Ludisia discolor groups, and hepatic glycogen was significantly increased (P<0.05) in a dose-dependent manner. The blood lactic acid was significantly decreased in high dose group, and weight loading swimming time was prolonged (P<0.05). The effects of Ludisia discolor is similar compared to Rhodiola. Conclusion The aqueous extract of Ludisia discolor has anti-fatigue action in mice. The mechanism might be related with the increased glycogen reserves, increased glucose aerobic decomposition, as well as reduced anaerobic glucolysis and reduced protein breakdown.
- aqueous extract of Ludisia discolor /
- exercise-indused fatigue /
- hepatic glycogen /
- blood lactic acid /
- blood urea nitrogen

[1]	石鹤坤,陈锦珊.红景天等单味中药抗疲劳的研究进展[J].中国医药导报,2012,9(4):9-10,13.
[2]	黄泽豪,沈贤娟.闽南民间药公石松的生药鉴定[J].亚热带植物科学,2013,42(4):288-292.
[3]	耿欣.红景天抗疲劳作用研究进展[J].中医药学报,2011,39(3):95-97.
[4]	龚梦鹃,王立为,刘新民.大小鼠游泳实验方法的研究概况[J].中国比较医学杂志,2005,15(5):311-314.
[5]	宫遴.运动中能量供应的基本理论[J].甘肃科技,2013,29(2):81-83.
[6]	Xu C,Lv J,Lo YM,et al.Effects of oat β-glucan on endurance exercise and its anti-fatigue properties in trained rats[J].Carbohydr Polym,2013,92(2): 1159-1165.
[7]	张宇,衣雪洁,王宝明.优秀单板U型场地滑雪运动员不同训练期部分生理生化指标监测分析[J].中国运动医学杂志,2012,31(1):62-65.
[8]	Dolny DG, Lemon PW. Effect of ambient temperature on protein breakdown during prolonged exercise [J]. Physiology, 1989, 64: 550-555.
[9]	辛甜,储智勇,栾洁,等. 铁皮石斛胚状体对大鼠抗疲劳能力的影响[J].药学实践杂志,2011,29(1):21-23.

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公石松水提液对小鼠运动能力与血清乳酸、尿素氮及肝糖原的影响

doi: 10.3969/j.issn.1006-0111.2016.05.005

解放军175医院/厦门大学附属东南医院药学科, 福建漳州 363000

基金项目: 解放军175医院青年苗圃基金资助项目（13Y017）

收稿日期: 2015-02-27
修回日期: 2015-12-24

关键词:

公石松水提液 /

运动性疲劳 /

肝糖原 /

血乳酸 /

血尿素氮

摘要: 目的探讨公石松水提液对抗小鼠运动性疲劳的作用及其机制。方法雄性小鼠随机分为5组：公石松水提液低、中、高剂量组[分别用公石松1.22、2.44、4.88 g/（kg·d）]、阳性对照组[红景天水提物2.34 g/（kg·d）]和空白对照组（等量蒸馏水），连续灌胃7 d 后，测定小鼠负重游泳时间及游泳90 min后血乳酸、血尿素氮及肝糖原指标。结果公石松水提液各组均可显著降低小鼠血尿素氮水平（P<0.05），增加肝糖原含量（P<0.05），呈量效关系；公石松水提液高剂量组可降低血乳酸，延长小鼠负重游泳时间（P<0.05）。其作用与阳性对照红景天水提物相当。结论公石松水提液具有抗小鼠运动性疲劳的作用，其作用机制可能通过提高肝糖原储备、增加葡萄糖的有氧分解、减少葡萄糖的无氧酵解及减少蛋白质的分解有关。

English Abstract

Effects of Ludisia discolor aqueous extract on exercise capacity, serum lactic acid, urea nitrogen and hepatic glycogen in mice

Department of Pharmacy, No.175 Hospital of PLA/Affiliated Southeast Hospital of Xiamen University, Zhangzhou, 363000 China

Received Date: 2015-02-27
Rev Recd Date: 2015-12-24

Keywords:

Abstract: Objective To investigate the effect against exercise-indused fatigue in mice by aqueous extract of Ludisia discolor and its mechanism. Methods Male mice were randomly divided into five groups including low, middle, high dose groups [aqueous extract of Ludisia discolor at dose of 1.22, 2.44, 4.88 g/(kg·d) respectively], positive control group [aqueous extract of Rhodiola, at dose of 2.34 g/(kg·d)] and control group (distilled water). After intragastric administration for seven days, mice were measured for loading swimming time, and were tested 90 minutes after loading swimming for blood urea nitrogen, blood lactic acid and hepatic glycogen levels. Results The blood urea nitrogen was significantly decreased (P<0.05) in aqueous extract of Ludisia discolor groups, and hepatic glycogen was significantly increased (P<0.05) in a dose-dependent manner. The blood lactic acid was significantly decreased in high dose group, and weight loading swimming time was prolonged (P<0.05). The effects of Ludisia discolor is similar compared to Rhodiola. Conclusion The aqueous extract of Ludisia discolor has anti-fatigue action in mice. The mechanism might be related with the increased glycogen reserves, increased glucose aerobic decomposition, as well as reduced anaerobic glucolysis and reduced protein breakdown.

引用本文:

Citation:

SHI Hekun, CHEN Kaijie, LIN Xiaofeng, CHEN Jinsan. Effects of Ludisia discolor aqueous extract on exercise capacity, serum lactic acid, urea nitrogen and hepatic glycogen in mice[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(5): 399-402. doi: 10.3969/j.issn.1006-0111.2016.05.005