HPLC法测定人参毛状根及不同人参样品中9种人参皂苷的含量

陈泠; 李春艳; 王政; 贾敏; 韩婷

doi:10.3969/j.issn.1006-0111.2016.04.007

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HPLC法测定人参毛状根及不同人参样品中9种人参皂苷的含量

陈泠, 李春艳, 王政, 贾敏, 韩婷

文章导航 > 药学实践与服务 > 2016 > 34(4): 313-317,353

陈泠, 李春艳, 王政, 贾敏, 韩婷. HPLC法测定人参毛状根及不同人参样品中9种人参皂苷的含量[J]. 药学实践与服务, 2016, 34(4): 313-317,353. doi: 10.3969/j.issn.1006-0111.2016.04.007

引用本文:

CHEN Ling, LI Chunyan, WANG Zheng, JIA Min, HAN Ting. Quantitation of nine ginsenosides in Panax ginseng hairy roots and other Panax ginseng plant components by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 313-317,353. doi: 10.3969/j.issn.1006-0111.2016.04.007

Citation:

CHEN Ling, LI Chunyan, WANG Zheng, JIA Min, HAN Ting. Quantitation of nine ginsenosides in Panax ginseng hairy roots and other Panax ginseng plant components by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(4): 313-317,353. doi: 10.3969/j.issn.1006-0111.2016.04.007

HPLC法测定人参毛状根及不同人参样品中9种人参皂苷的含量

doi: 10.3969/j.issn.1006-0111.2016.04.007

第二军医大学药学院生药学教研室, 上海 200433

基金项目: 第二军医大学“本科学员创新实践能力孵化基地”项目（FH2015098）；国家自然基金（81473301）.

Quantitation of nine ginsenosides in Panax ginseng hairy roots and other Panax ginseng plant components by HPLC

Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

摘要: 目的采用HPLC法测定不同人参样品中9种人参皂苷（Rc、Rb₁、Rb₂、Re、Rd、Rg₁、Rg₂、Rg₃和Rh₂）的含量。方法色谱条件：Zorbax SB C₁₈柱（4.6 mm×250 mm，5μm），保护柱Extend-C₁₈柱（4.6 mm×12.5 mm，5μm）；以乙腈-水为流动相，梯度洗脱；流速：1.0 ml/min；检测波长：203 nm；柱温：35℃。结果 9种人参皂苷Rg₁、Rb₁、Re、Rc、Rg₂、Rh₂、Rg₃、Rb₂和Rd在120 min内基线分离。方法学表明其线性关系良好，精密度、稳定性和重复性RSD均小于2.0%，加样回收率在98.3%～102%之间。测得人参叶和人参须根中的总皂苷含量最高，分别为48.9、23.6 mg/g；人参毛状根中的总皂苷与人参主根和人参果的总皂苷含量差别不大，为7.47 mg/g。结论该法准确性高，操作简便、快速，重复性好，精密度高，可用于不同人参样品中9种人参皂苷的含量测定。
- 人参 /
- 人参饮片 /
- 毛状根 /
- 人参皂苷 /
- 高效液相色谱法
Abstract: Objective To establish an HPLC method for the quantitation of nine ginsenosides (Rc,Rb₁,Rb₂,Re,Rd,Rg₁,Rg₂,Rg₃ and Rh₂) in Panax ginseng samples. Methods An HPLC method was developed to determine the quantities of the nine ginsenosides. The determination was performed on a Zorbax SB C₁₈ column (4.6 mm×250 mm,5 μm) with an Extend-C₁₈ guard column (4.6 mm×12.5 mm,5 μm) at 35℃. The mobile phase was a multi-step acetonitrile-water gradient run at a flow rate of 1.0 ml/min. The detection wavelength was 203 nm. Results The nine ginsenosides were baseline separated within 120 min. The method had good linearity, precision, stability and reproducibility with RSDs all less than 2.0%. The sample recoveries were between 98.3% to 102%. The quantity of total saponins in leaves and fibrous roots of Panax ginseng, which were measured as 48.9 mg/g and 23.6 mg/g, respectively, were higher than those in the other plant components. The amounts of total saponins in Panax ginseng hairy roots were similar to those in taproots and fruits of Panax ginseng, which was 7.47 mg/g. Conclusion The established HPLC method is accurate, simple, rapid, precise and reproducible and could be used for the quantitation of these nine ginsenosides in Panax ginseng samples.
- Panax ginseng decoction pieces /
- hairy root /
- Panax ginseng /
- ginsenoside /
- HPLC

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HPLC法测定人参毛状根及不同人参样品中9种人参皂苷的含量

doi: 10.3969/j.issn.1006-0111.2016.04.007

第二军医大学药学院生药学教研室, 上海 200433

基金项目: 第二军医大学“本科学员创新实践能力孵化基地”项目（FH2015098）；国家自然基金（81473301）.

收稿日期: 2016-01-26
修回日期: 2016-05-03

关键词:

人参 /

人参饮片 /

毛状根 /

人参皂苷 /

高效液相色谱法

摘要: 目的采用HPLC法测定不同人参样品中9种人参皂苷（Rc、Rb₁、Rb₂、Re、Rd、Rg₁、Rg₂、Rg₃和Rh₂）的含量。方法色谱条件：Zorbax SB C₁₈柱（4.6 mm×250 mm，5μm），保护柱Extend-C₁₈柱（4.6 mm×12.5 mm，5μm）；以乙腈-水为流动相，梯度洗脱；流速：1.0 ml/min；检测波长：203 nm；柱温：35℃。结果 9种人参皂苷Rg₁、Rb₁、Re、Rc、Rg₂、Rh₂、Rg₃、Rb₂和Rd在120 min内基线分离。方法学表明其线性关系良好，精密度、稳定性和重复性RSD均小于2.0%，加样回收率在98.3%～102%之间。测得人参叶和人参须根中的总皂苷含量最高，分别为48.9、23.6 mg/g；人参毛状根中的总皂苷与人参主根和人参果的总皂苷含量差别不大，为7.47 mg/g。结论该法准确性高，操作简便、快速，重复性好，精密度高，可用于不同人参样品中9种人参皂苷的含量测定。

English Abstract

Quantitation of nine ginsenosides in Panax ginseng hairy roots and other Panax ginseng plant components by HPLC

Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received Date: 2016-01-26
Rev Recd Date: 2016-05-03

Keywords:

Abstract: Objective To establish an HPLC method for the quantitation of nine ginsenosides (Rc,Rb₁,Rb₂,Re,Rd,Rg₁,Rg₂,Rg₃ and Rh₂) in Panax ginseng samples. Methods An HPLC method was developed to determine the quantities of the nine ginsenosides. The determination was performed on a Zorbax SB C₁₈ column (4.6 mm×250 mm,5 μm) with an Extend-C₁₈ guard column (4.6 mm×12.5 mm,5 μm) at 35℃. The mobile phase was a multi-step acetonitrile-water gradient run at a flow rate of 1.0 ml/min. The detection wavelength was 203 nm. Results The nine ginsenosides were baseline separated within 120 min. The method had good linearity, precision, stability and reproducibility with RSDs all less than 2.0%. The sample recoveries were between 98.3% to 102%. The quantity of total saponins in leaves and fibrous roots of Panax ginseng, which were measured as 48.9 mg/g and 23.6 mg/g, respectively, were higher than those in the other plant components. The amounts of total saponins in Panax ginseng hairy roots were similar to those in taproots and fruits of Panax ginseng, which was 7.47 mg/g. Conclusion The established HPLC method is accurate, simple, rapid, precise and reproducible and could be used for the quantitation of these nine ginsenosides in Panax ginseng samples.