四逆汤治疗甲状腺功能减退症的血清代谢组学研究

贾雨婷; 靳雨晨; 谭光国

doi:10.3969/j.issn.1006-0111.2016.03.011

四逆汤治疗甲状腺功能减退症的血清代谢组学研究

doi: 10.3969/j.issn.1006-0111.2016.03.011

1.
第四军医大学学员一旅, 陕西西安 710032
2.
第四军医大学药学系药物分析学教研室, 陕西西安 710032

基金项目: 国家自然科学基金项目(81402888);全军医学科技青年培育项目(13QNT197);陕西自然科学基金项目(2015JM8458)

Study on Sini decoction in treatment of hypothyroidism by metabonomic method

1.
Fourth Military Medical University, First Cadets Brigade, Xi'an 710032, China
2.
Fourth Military Medical University, Department of Pharmaceutical Analysis, School of Pharmacy, Xi'an 710032, China

摘要: 目的利用血清代谢组学鉴定甲状腺功能减退症(甲减)相关的代谢变化,评价四逆汤治疗甲减的作用。方法将24只大鼠分为假手术组、模型组和四逆汤治疗组,采用甲状腺切除诱导甲减模型,给予4周相应的治疗后,取大鼠血清进行超高效液相色谱-四极杆-飞行时间质谱仪(UPLC-Q-TOFMS)分析,结合模式识别分析各组间代谢物差异,评价四逆汤的治疗作用。结果血清代谢组学分析鉴定了9种甲减相关的生物标志物,涉及三羧酸循环、苯丙氨酸代谢、鞘脂代谢和磷脂代谢,同时数据显示四逆汤对甲减有改善治疗作用。结论四逆汤能够通过调节失衡的三羧酸循环、苯丙氨酸代谢和磷脂代谢而发挥治疗甲减的作用。
- 甲减 /
- 超高效液相色谱-四极杆-飞行时间质谱仪 /
- 四逆汤 /
- 代谢组学
Abstract: Objective To evaluate therapeutic effects of Sini decoction (SND) on hypothyroidism based on serum metabonomics method. Methods Twenty-four rats were randomly divided into three groups including sham group, surgery-induced hypothyroid model group and SND-treated group. The SND-treated group was given SND for 4 weeks. After 4 weeks, the serum samples of each rat were collected and analyzed by UHPLC-Q-TOFMS, pattern recognition analysis of metabolomics differences among the groups and therapeutic effects of SND were evaluated. Results Total of 9 metabolites were identified through serum metabonomics analysis. It was showed that there was a possible mechanism that hypothyroidism was mainly involved in citrate cycle, phenylalanine metabolism, sphingolipid metabolism and phospholipid metabolism and the damage of hypothyroidism was reversed by SND. Conclusion SND administration could provide satisfactory effects on hypothyroidism through partially regulating the perturbed citrate cycle, phenylalanine metabolism and phospholipid metabolism.
- hypothyroidism /
- UHPLC-Q-TOFMS /
- Sini decoction /
- metabonomics

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四逆汤治疗甲状腺功能减退症的血清代谢组学研究

doi: 10.3969/j.issn.1006-0111.2016.03.011

Study on Sini decoction in treatment of hypothyroidism by metabonomic method

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四逆汤治疗甲状腺功能减退症的血清代谢组学研究

doi: 10.3969/j.issn.1006-0111.2016.03.011

1. 第四军医大学学员一旅, 陕西西安 710032

2. 第四军医大学药学系药物分析学教研室, 陕西西安 710032

English Abstract

Study on Sini decoction in treatment of hypothyroidism by metabonomic method

1. Fourth Military Medical University, First Cadets Brigade, Xi'an 710032, China

2. Fourth Military Medical University, Department of Pharmaceutical Analysis, School of Pharmacy, Xi'an 710032, China

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四逆汤治疗甲状腺功能减退症的血清代谢组学研究

doi: 10.3969/j.issn.1006-0111.2016.03.011

Study on Sini decoction in treatment of hypothyroidism by metabonomic method

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出版历程

四逆汤治疗甲状腺功能减退症的血清代谢组学研究

doi: 10.3969/j.issn.1006-0111.2016.03.011

1. 第四军医大学学员一旅, 陕西 西安 710032 2. 第四军医大学药学系药物分析学教研室, 陕西 西安 710032

English Abstract

Study on Sini decoction in treatment of hypothyroidism by metabonomic method

1. Fourth Military Medical University, First Cadets Brigade, Xi'an 710032, China 2. Fourth Military Medical University, Department of Pharmaceutical Analysis, School of Pharmacy, Xi'an 710032, China

全文HTML

目录

1. 第四军医大学学员一旅, 陕西西安 710032

2. 第四军医大学药学系药物分析学教研室, 陕西西安 710032

1. Fourth Military Medical University, First Cadets Brigade, Xi'an 710032, China

2. Fourth Military Medical University, Department of Pharmaceutical Analysis, School of Pharmacy, Xi'an 710032, China