Combination of wogonin with 5-fluorouracil exhibits antagonistic effect on human Hep-G2 hepatocellular carcinoma cells
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摘要: 目的 探讨汉黄芩素(wogonin)联合氟尿嘧啶(5-FU)对人肝癌细胞Hep-G2生长活性的影响。 方法 实验分汉黄芩素组、5-FU组、汉黄芩素+5-FU组和空白对照组,采用MTT法观察药物对肿瘤细胞体外生长活性的影响,采用流式细胞仪分析肿瘤细胞凋亡率的变化。 结果 MTT实验结果显示汉黄芩素(5、10、20、40 μmol/L)作用24、48 h后对肿瘤细胞具有一定的增殖抑制作用(P<0.05);5-FU(5、10、20、40 mg/L)作用24、48 h后对肿瘤细胞增殖有明显的抑制作用(P<0.05);与单用组对肿瘤细胞的抑制作用相比,汉黄芩素联合5-FU组的抗肿瘤作用呈相互拮抗作用(P<0.05);汉黄芩素联合5-FU给药48 h后,联合指数CI值呈现剂量依赖性,CI值随汉黄芩素浓度的加大而增大,说明随汉黄芩素浓度的加大,其拮抗5-FU抗肿瘤效应的作用也越来越明显(P<0.05)。 结论 汉黄芩素具有一定的抗肿瘤作用,但可拮抗5-FU的抗肿瘤作用,具体机制有待进一步研究。
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关键词:
- 汉黄芩素 /
- 氟尿嘧啶 /
- 拮抗作用 /
- 肝癌细胞Hep-G2
Abstract: Objective To investigate the antagonistic effect of wogonin in combination with 5-fluorouracil (5-FU) on the proliferation of human Hep-G2 hepatocellular carcinoma cells. Methods Human hepatocellular Hep-G2 cells were divided into experimental group (wogonin group, 5-FU group, wogonin + 5-FU group) and control group. MTT method was used to evaluate tumor cell proliferation in vitro, flow cytometry analysis was used to evaluate tumor cell apoptosis. Results The results showed that the wogonin inhibited the proliferation of tumor cells at the concentrations of 5, 10, 20 and 40 μmol/L after 24 h and 48 h treatment respectively (P<0.05); 5-FU also inhibited the proliferation of tumor cells at the concentrations of 5, 10, 20 and 40 mg/L after 24 h and 48 h treatment respectively (P<0.05). However when wogonin was combined with 5-FU (wogonin+5-FU group), an antagonistic effect was observed on tumor cell proliferation (P<0.05). When cells were treated by wogonin+5-FU for 48 h, the combined index (CI) value slowed a dose-dependent antagonistic effect (P<0.05). Conclusion Wogonin has anti-tumor effect. However when wogonin was combined with 5-FU, an obvious antagonistic effect on 5-FU's anti-tumor action was observed. The underlying mechanism deserves further study.-
Key words:
- wogonin /
- fluorouracil /
- antagonism /
- hepatocellular carcinoma cell Hep-G2
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