Determination of asiatic acid in the root of Actinidia valvata by HPLC
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摘要: 目的 建立猫人参药材中积雪草酸的含量测定方法。 方法 采用高效液相色谱法,色谱柱为Agilent HC-C18柱(4.6 mm×250 mm,5.0 μm),流动相为乙腈(30 mmol/L)-醋酸铵水溶液(35:65),流速1.0 ml/min,柱温25 ℃,紫外检测波长210 nm,进样量25 μl,运行时间35 min。 结果 积雪草酸与周围干扰峰达到基线分离,线性范围25.3~506.0 μg/ml, r=0.999 6;日内及日间精密度均<3% (n=5),平均回收率为99.4%(RSD=1.9%, n=6),猫人参中积雪草酸的含量为0.751 mg/g。 结论 该法简便快捷、测定结果准确、实用性强,可用于猫人参药材中积雪草酸的含量测定。Abstract: Objective To establish a method for the determination of asiatic acid in the root of Actinidia valvata. Methods HPLC-VWD was used in the analysis. The column was Agilent HC-C18 (4.6 mm×250 mm,5μm);the mobile phase was acetonitrile: (30 mmol/L) acetic acid amine solution (35:65); the flow rate was 1.0 ml/min; the temperature of column was 25 ℃; the detection wavelength was set at 210 nm; the injection volume was 25 μl; the running time was 35 min. Results Asiatic acid was separated with interference in baseline. The linear range was 25.30-506.0 μg/ml with linear correlation of 0.999 6 for asiatic acid. The result of intra-day and inter-day precisions were both within 5% (n=3), and the average recovery was 99.4% with RSD 1.9% (n=6). Conclusion The method was simple, rapid, accurate and convenient for quality control of asiatic acid in the root of Actinidia valvata.
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Key words:
- Actinidia valvata /
- asiatic acid /
- determination /
- HPLC
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