Determination of the polysaccharide in Verdihong paint
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摘要: 目的 建立维地红涂剂中多糖的含量测定方法。 方法 用无水葡萄糖为对照品,0.2%蒽酮-硫酸试液为染色剂,紫外分光光度法测定,测定波长620 nm。 结果 多糖在1.9~34.2 μg/ml范围内线性关系良好,Y=0.033X-0.006 7(r=0.999 9),回收率为98.6%,RSD=1.06%(n=6)。 结论 所建方法便捷、重现性好,适用于维地红涂剂中多糖的含量测定。Abstract: Objective To establish the determination method of the polysaccharide in Verdihong paint. Methods The anhydrous dextrose was used as the control, 0.2% anthrone-sulfuric acid as the stain, the content of polysaccharide was determined by ultraviolet spectrophotometer at the wavelength of 620 nm. Results The polysaccharide curve was linear within a range of 1.9~34.2 μg/ml, linear equation was Y=0.033 X-0.006 7 (r=0.999 9), the average recovery was 98.6% with RSD of 1.06% (n=6). Conclusion This method was convenient, stable and coud be used to assay content of the polysaccharide in Verdihong paint.
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Key words:
- Verdihong paint /
- polysaccharide /
- content determination
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