应用中空纤维测定法评价藤甲酰苷对人癌细胞生长的抑制作用

邓晶晶; 纪圣君; 陈烨; 林晓琳; 王蕊; 徐利锋

doi:10.3969/j.issn.1006-0111.2014.04.007

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应用中空纤维测定法评价藤甲酰苷对人癌细胞生长的抑制作用

邓晶晶, 纪圣君, 陈烨, 林晓琳, 王蕊, 徐利锋

文章导航 > 药学实践与服务 > 2014 > 32(4): 266-269

邓晶晶, 纪圣君, 陈烨, 林晓琳, 王蕊, 徐利锋. 应用中空纤维测定法评价藤甲酰苷对人癌细胞生长的抑制作用[J]. 药学实践与服务, 2014, 32(4): 266-269. doi: 10.3969/j.issn.1006-0111.2014.04.007

引用本文:

DENG Jingjing, JI Shengjun, CHEN Ye, LIN Xiaolin, WANG Rui, XU Lifeng. Evaluation of the inhibitory effect of garcinia glycosides on growth of human tumor cells by hollow fiber assay[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(4): 266-269. doi: 10.3969/j.issn.1006-0111.2014.04.007

Citation:

DENG Jingjing, JI Shengjun, CHEN Ye, LIN Xiaolin, WANG Rui, XU Lifeng. Evaluation of the inhibitory effect of garcinia glycosides on growth of human tumor cells by hollow fiber assay[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(4): 266-269. doi: 10.3969/j.issn.1006-0111.2014.04.007

应用中空纤维测定法评价藤甲酰苷对人癌细胞生长的抑制作用

doi: 10.3969/j.issn.1006-0111.2014.04.007

辽宁大学药学院, 辽宁沈阳 110036

基金项目: 国家"十一五"规划"重大新药创制"重大科技专项(2009ZX09103-030).

Evaluation of the inhibitory effect of garcinia glycosides on growth of human tumor cells by hollow fiber assay

School of Pharmacy, Liaoning University, Shenyang 110036, China

摘要: 目的应用中空纤维测定法评价藤甲酰苷(garcinia glycosides,GG)对8种人癌细胞的体内生长的抑制作用,通过裸鼠体内异位移植瘤实验,验证中空纤维测定法在抗肿瘤药效学研究中的可靠性。方法采用中空纤维测定法,将载有肿瘤细胞的中空纤维管植入NOD/SCID 小鼠背部皮下,给药结束后取出中空纤维管,应用二苯基溴化四氮唑蓝(MTT)比色法检测GG对各种肿瘤细胞在体内的抑瘤率;选取HL-60及B16人癌细胞,异位移植于BALb/c裸鼠右下肢外侧皮下,以环磷酰胺(CTX)为阳性对照,各组连续给药10 d,于给药结束24 h后解剖小鼠,取出瘤块,计算CTX及GG的抑瘤率。结果应用中空纤维测定法及裸鼠体内异位移植瘤法测得GG高剂量组8 mg/(kg·d)、中剂量组4 mg/(kg·d)能明显抑制HL-60及B16人癌细胞在裸鼠体内的生长,实验测得结果与溶剂对照组比较,有显著性差异(P<0.01)。结论应用中空纤维测定法测定样品GG对肿瘤细胞的生长抑制作用,实验结果与裸鼠体内异位移植瘤实验结果基本一致。该模型节约成本、效率提高、实验结果准确可靠,为GG的后续研究提供了指导和可靠证据。
- 藤甲酰苷 /
- 中空纤维测定法 /
- HL-60 /
- B16 /
- MTT比色法
Abstract: Objective To evaluated the inhibitory effect of garcinia glycosides on growth of 8 kinds of human tumor cells in vivo by hollow fiber assay and confirm the reliability of hollow fiber assay in anticancer effect by the nude mice xenograft test. Methods Hollow fibers containing tumor cells were inserted underneath the skin of the NOD/SCID mice.The fibers were collected from the mice on the day after the administration and subjected to the stable endpoint MTT assay.The tumor cells of HL-60 and B16 were subcutaneously implanted into the right flank of BALb /c nude mice.The positive control group was treated with cyclophosphamide.Each group was administered for 10 days.24 hours after the last administration, the mice were sacrificed and the tumors were excised and weighted, the inhibition rate of tumor growth was calculated. Results The high-dose group of 8 mg/(kg·d), middle dose group of 4 mg/(kg·d) of garcinia glycosides were measured by hollow fiber assay and nude mice test significantly inhibited the in vivo growth of HL-60 and B16 comparing with those in the solvent control group(P<0.01). Conclusion As a new model by hollow fiber assay to evaluate the inhibitory effect of garcinia glycosides, the test results were basically the same with nude mice test results.It made the experiment more rapidly, accurately and economically.An instruction and reliable evidence for follow-up study of garcinia glycosides was provided in this study.
- garcinia glycosides /
- hollow fiber assay /
- HL-60 /
- B16 /
- MTT

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应用中空纤维测定法评价藤甲酰苷对人癌细胞生长的抑制作用

doi: 10.3969/j.issn.1006-0111.2014.04.007

辽宁大学药学院, 辽宁沈阳 110036

基金项目: 国家"十一五"规划"重大新药创制"重大科技专项(2009ZX09103-030).

收稿日期: 2013-03-22
修回日期: 2013-09-14

关键词:

藤甲酰苷 /

中空纤维测定法 /

HL-60 /

B16 /

MTT比色法

摘要: 目的应用中空纤维测定法评价藤甲酰苷(garcinia glycosides,GG)对8种人癌细胞的体内生长的抑制作用,通过裸鼠体内异位移植瘤实验,验证中空纤维测定法在抗肿瘤药效学研究中的可靠性。方法采用中空纤维测定法,将载有肿瘤细胞的中空纤维管植入NOD/SCID 小鼠背部皮下,给药结束后取出中空纤维管,应用二苯基溴化四氮唑蓝(MTT)比色法检测GG对各种肿瘤细胞在体内的抑瘤率;选取HL-60及B16人癌细胞,异位移植于BALb/c裸鼠右下肢外侧皮下,以环磷酰胺(CTX)为阳性对照,各组连续给药10 d,于给药结束24 h后解剖小鼠,取出瘤块,计算CTX及GG的抑瘤率。结果应用中空纤维测定法及裸鼠体内异位移植瘤法测得GG高剂量组8 mg/(kg·d)、中剂量组4 mg/(kg·d)能明显抑制HL-60及B16人癌细胞在裸鼠体内的生长,实验测得结果与溶剂对照组比较,有显著性差异(P<0.01)。结论应用中空纤维测定法测定样品GG对肿瘤细胞的生长抑制作用,实验结果与裸鼠体内异位移植瘤实验结果基本一致。该模型节约成本、效率提高、实验结果准确可靠,为GG的后续研究提供了指导和可靠证据。

English Abstract

Evaluation of the inhibitory effect of garcinia glycosides on growth of human tumor cells by hollow fiber assay

School of Pharmacy, Liaoning University, Shenyang 110036, China

Received Date: 2013-03-22
Rev Recd Date: 2013-09-14

Keywords:

Abstract: Objective To evaluated the inhibitory effect of garcinia glycosides on growth of 8 kinds of human tumor cells in vivo by hollow fiber assay and confirm the reliability of hollow fiber assay in anticancer effect by the nude mice xenograft test. Methods Hollow fibers containing tumor cells were inserted underneath the skin of the NOD/SCID mice.The fibers were collected from the mice on the day after the administration and subjected to the stable endpoint MTT assay.The tumor cells of HL-60 and B16 were subcutaneously implanted into the right flank of BALb /c nude mice.The positive control group was treated with cyclophosphamide.Each group was administered for 10 days.24 hours after the last administration, the mice were sacrificed and the tumors were excised and weighted, the inhibition rate of tumor growth was calculated. Results The high-dose group of 8 mg/(kg·d), middle dose group of 4 mg/(kg·d) of garcinia glycosides were measured by hollow fiber assay and nude mice test significantly inhibited the in vivo growth of HL-60 and B16 comparing with those in the solvent control group(P<0.01). Conclusion As a new model by hollow fiber assay to evaluate the inhibitory effect of garcinia glycosides, the test results were basically the same with nude mice test results.It made the experiment more rapidly, accurately and economically.An instruction and reliable evidence for follow-up study of garcinia glycosides was provided in this study.

引用本文:

Citation:

DENG Jingjing, JI Shengjun, CHEN Ye, LIN Xiaolin, WANG Rui, XU Lifeng. Evaluation of the inhibitory effect of garcinia glycosides on growth of human tumor cells by hollow fiber assay[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(4): 266-269. doi: 10.3969/j.issn.1006-0111.2014.04.007