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色谱-质谱联用技术测定人体内类固醇激素的研究进展

钱跹,  赵亮,  吕磊,  张海,  李悦悦,  张国庆

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文章导航 > 药学实践与服务  > 2014 >  32(3): 176-180,190
钱跹, 赵亮, 吕磊, 张海, 李悦悦, 张国庆. 色谱-质谱联用技术测定人体内类固醇激素的研究进展[J]. 药学实践与服务, 2014, 32(3): 176-180,190. doi: 10.3969/j.issn.1006-0111.2014.03.004
引用本文: 钱跹, 赵亮, 吕磊, 张海, 李悦悦, 张国庆. 色谱-质谱联用技术测定人体内类固醇激素的研究进展[J]. 药学实践与服务, 2014, 32(3): 176-180,190. doi: 10.3969/j.issn.1006-0111.2014.03.004
shu
QIAN Xian, ZHAO Liang, LU Lei, ZHANG Hai, LI Yueyue, ZHANG Guoqing. Research progress in steroid hormone analysis in vivo by chromatography-mass spectrometry[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(3): 176-180,190. doi: 10.3969/j.issn.1006-0111.2014.03.004
Citation: QIAN Xian, ZHAO Liang, LU Lei, ZHANG Hai, LI Yueyue, ZHANG Guoqing. Research progress in steroid hormone analysis in vivo by chromatography-mass spectrometry[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(3): 176-180,190. doi: 10.3969/j.issn.1006-0111.2014.03.004
shu

色谱-质谱联用技术测定人体内类固醇激素的研究进展

doi: 10.3969/j.issn.1006-0111.2014.03.004

  • 第二军医大学东方肝胆外科医院药材科, 上海 200438

Research progress in steroid hormone analysis in vivo by chromatography-mass spectrometry

  • Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China

  • 摘要: 类固醇激素是高效能的生物化学物质,在多种生理活动中发挥显著的调节作用,但在体内的含量极低,因此对其进行准确定量具有重要意义。在各种检测方法中,色谱-质谱联用技术因具有高效、快速、灵敏的优点而得以广泛应用。笔者综述近年来色谱-质谱联用技术在内源性类固醇激素测定中的应用,为进一步的临床研究提供参考依据。
    Abstract: Steroid hormones were chemical compounds of high efficiency, which played a significant role in various physiological activities. However, the concentrations of hormones in vivo were extremely low, so it was very important for their accurate quantification. Chromatography-mass spectrometry technology was widely employed because of advances in high efficient, rapid and sensitive. The application of steroid hormone analysis by chromatography-mass spectrometry was reviewed in this paper, which could provide reference for furthuer clinical research.
  • [1] Holst JP,Soldin OP,Guo T,et al. Steroid hormones: relevance and measurement in the clinical laboratory[J].Clin Lab Med,2004,24(1):105-118.
    [2] Dorgan JF,Fears TR,McMahon RP,et al. Measurement of steroid sex hormones in serum: a comparison of radioimmunoassay and mass spectrometry[J].Steroids,2002,67(3-4):151-158.
    [3] Carter GD,Carter R,Jones J,et al. How accurate are assays for 25-hydroxyvitamin D-Data from the international vitamin D external quality assessment scheme[J].Clin Chem,2004,50(11):2195-2197.
    [4] Hollis BW,Kamerud JQ,Kurkowski A,et al. Quantification of circulating 1,25-dihydroxyvitamin D by radioimmunoassay with 125 I-labeled tracer[J].Clin Chem,1996,42(4):586-592.
    [5] Hsing AW,Stanczyk FZ,Belanger A,et al. Reproducibility of serum sex steroid assays in men by RIA and mass spectrometry[J].Cancer Epidemiol Biomarkers Prev,2007,16(5):1004-1008.
    [6] Poulson K,Sancho J,Haber E. A simplified radioimmunoassay for plasma aldosterone employing an antibody of unique specificity[J].Clin Immunol,1974,2(3):373-380.
    [7] Schioler V,Thode J. Six direct radioimmunoassays of estradiol evaluated[J].Clin Chem,1988,34(5):949-952.
    [8] Xu X,Roman JM,Issaq HJ,et al.Quantitative measurement of endogenous estrogens and estrogen metabolites in human serum by liquid chromatography-tandem mass spectrometry[J].Anal Chem,2007,79(20):7813-7821.
    [9] Santen RJ,Demers L,Ohorodnik S,et al. Superiority of gas chromatography/tandem mass spectrometry assay (GC/MS/MS) for estradiol for monitoring of aromatase inhibitor therapy[J].Steroids,2007,72(8):666-671.
    [10] Wolthers BG,Kraan GP. Clinical applications of gas chromatography and gas chromatographymass spectrometry of steroids[J].J Chromatogr A,1999,843(1-2):247-274.
    [11] Draisci R,Palleschi L,Ferretti E,et al.Quantitation of anabolic hormones and their metabolites in bovine serum and urine by liquid chromatography-tandem mass spectrometry[J].J Chromatogr A,2000,870(1-2):511-522.
    [12] Guo T,Chan M,Soldin SJ. Steroid profiles using liquid chromatography-tandem mass spectrometry with atmospheric pressure photoionization source[J].Arch Pathol Lab Med,2004,128(4):469-475.
    [13] Guo T,Gu J,Soldin OP,et al.Rapid measurement of estrogens and their metabolites in human serum by liquid chromatographytandem mass spectrometry without derivatization[J].Clin Biochem,2008,41(9):736-741.
    [14] Nelson RE,Grebe SK,Kane DJ,et al. Liquid chromatography-tandem mass spectrometry assay for simultaneous measurement of estradiol and estrone in human plasma[J].Clin Chem,2004,50(2):373-384.
    [15] Guo T,Taylor RL,Singh RJ,et al. Simultaneous determination of 12 steroids by isotope dilution liquid chromatography-photospray ionization tandem mass spectrometry[J].Clin Chim Acta,2006,372(1-2):76-82.
    [16] Andrew R. Clinical measurement of steroid metabolism[J].Best Pract Res Clin Endocrinol Metab,2001,15(1):1-16.
    [17] Abraham GE. Solid-phase radioimmunoassay of 17β-estradiol[J].J Clin Endocrinol Metab, 1969,29(6):866-870.
    [18] Rahkonena PK,Huhtinenb K,Poutanenb M,et al. Fast and sensitive liquid chromatography-mass spectrometry assay for seven androgenic and progestagenic steroids in human serum[J].J Steroid Biochem Mol Biol,2011,127(3-5):396-404.
    [19] Rothman MS,Carlson NE,Xu M,et al. Reexamination of testosterone, dihydrotestosterone, estradiol and estrone levels across the menstrual cycle and in postmenopausal women measured by liquid chromatography-tandem mass spectrometry[J].Steroids,2011,76(1-2):177-182.
    [20] Kushnir MM,Rockwood AL,Roberts WL,et al. Development and performance evaluation of a tandem mass spectrometry assay for 4 adrenal steroids[J].Clin Chem,2006,52(8):1559-1567.
    [21] Carvalho VM,Nakamura OH,Vieira JG. Simultaneous quantitation of seven endogenous C-21 adrenal steroids by liquid chromatography tandem mass spectrometry in human serum[J].J Chromatogr B Analyt Technol Biomed Life Sci,2008,872(1-2):154-161.
    [22] McWhinney BC,Briscoe SE,Ungerer JP. Pretorius CJ.Measurement of cortisol, cortisone, prednisolone, dexamethasone and 11-deoxycortisol with ultra high performance liquid chromatography-tandem mass spectrometry: application for plasma, plasma ultrafiltrate, urine and saliva in a routine laboratory[J].J Chromatogr B Analyt Technol Biomed Life Sci,2010,878(28):2863-2869.
    [23] Rauh M,Gröschl M,Rascher W,et al.Automated, fast and sensitive quantification of 17 alpha-hydroxy-progesterone, androstenedione and testosteroneby tandem mass spectrometry with on-line extraction[J].Steroids,2006,71(6):450-458.
    [24] Shackleton CH. Mass spectrometry: application to steroid and peptide research[J].Endocr Rev,1985,6(3):441-486.
    [25] Hansen M,Jacobsen NW,Nielsen FK,et al. Determination of steroid hormones in blood by GC-MS/MS[J]. Anal Bioanal Chem,2011,400(10):3409-3417.
    [26] Courant F,Aksglaede L,Antignac JP,et al. Assessment of circulating sex steroid levels in prepubertal and pubertal boys and girls by a novel ultrasensitive gas chromatography-tandem mass spectrometry method [J].J Steroid Biochem Mol Biol,2010,95(1):82-92.
    [27] Steven JS,Soldin OP. Steroid hormone analysis by tandem mass spectrometry[J].Clin Chem,2009,55(6):1061-1066.
    [28] McDonald JG,Matthew S,Richard J. Steroid profiling by gas chromatography-mass spectrometry and high performance liquid chromatography-mass spectrometry for adrenal diseases[J].Horm Cancer,2011,2(6):324-332.
    [29] Kushnir MM,Rockwood AL,William L,et al. Liquid chromatography tandem mass spectrometry for analysis of steroids in clinical laboratories[J].Clin Biochem,2011,44 (1):77-88.
    [30] Koal T,Schmiederer D,Rauh M. Standardized LC-MS/MS based steroid hormone profile-analysis[J].J Steroid Biochem Mol Biol,2012,129(3-5):129-138.
    [31] Naessen T,Sjogren U,Bergquist J,et al. Endogenous steroids measured by high-specificity liquid chromatography-tandem mass spectrometry and prevalent cardiovascular disease in 70-year-old men and women[J].J Clin Endocrinol Metab,2010,95(4):1889-1897.
    [32] Rossi C,Calton L,Hammond G,et al. Serum steroid profiling for congenital adrenal hyperplasia using liquid chromatography-tandem mass spectrometry[J].Clin Chim Acta,2010,411(3-4):222-228.
    [33] Kulle AE,Riepe FG,Melchior D,et al. A novel ultrapressure liquid chromatography tandem mass spectrometry method for the simultaneous determination of androstenedione, testosterone, and dihydrotestosterone in pediatric blood samples: age-and sex-specific reference data[J].J Clin Endocrinol Metab,2010,95(5):2399-2409.
    [34] Rousu T,Tolonen A. Comparison of unit resolution SRM and TOF-MS at 12,000 mass resolution for quantitative bioanalysis of 11 steroids from human plasma[J].Bioanalysis,2012,4(5):555-563.
    [35] Ceglarek U,Kortz L,Leichtle A,et al. Rapid quantification of steroid patterns in human serum by on-line solid phase extraction combined with liquid chromatography-triple quadrupole linear ion trap mass spectrometry[J].Clin Chim Acta,2009,401(1-2):114-118.
    [36] Higashi T,Shimada K. Derivatization of neutral steroids to enhance their detection characteristics in liquid chromatography-mass spectrometry[J].Anal Bioanal Chem,2004,378(4):875-882.
    [37] Maidana P, Bruno OD, Mesch V. A critical analysis of cortisol measurements: an update[J]. Medicina (B Aires),2013,73(6):579-584.
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  • 收稿日期:  2013-08-28
  • 修回日期:  2014-01-14

色谱-质谱联用技术测定人体内类固醇激素的研究进展

doi: 10.3969/j.issn.1006-0111.2014.03.004
  • 第二军医大学东方肝胆外科医院药材科, 上海 200438
  • 收稿日期: 2013-08-28
  • 修回日期: 2014-01-14

摘要: 类固醇激素是高效能的生物化学物质,在多种生理活动中发挥显著的调节作用,但在体内的含量极低,因此对其进行准确定量具有重要意义。在各种检测方法中,色谱-质谱联用技术因具有高效、快速、灵敏的优点而得以广泛应用。笔者综述近年来色谱-质谱联用技术在内源性类固醇激素测定中的应用,为进一步的临床研究提供参考依据。

English Abstract

钱跹, 赵亮, 吕磊, 张海, 李悦悦, 张国庆. 色谱-质谱联用技术测定人体内类固醇激素的研究进展[J]. 药学实践与服务, 2014, 32(3): 176-180,190. doi: 10.3969/j.issn.1006-0111.2014.03.004
引用本文: 钱跹, 赵亮, 吕磊, 张海, 李悦悦, 张国庆. 色谱-质谱联用技术测定人体内类固醇激素的研究进展[J]. 药学实践与服务, 2014, 32(3): 176-180,190. doi: 10.3969/j.issn.1006-0111.2014.03.004
shu
QIAN Xian, ZHAO Liang, LU Lei, ZHANG Hai, LI Yueyue, ZHANG Guoqing. Research progress in steroid hormone analysis in vivo by chromatography-mass spectrometry[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(3): 176-180,190. doi: 10.3969/j.issn.1006-0111.2014.03.004
Citation: QIAN Xian, ZHAO Liang, LU Lei, ZHANG Hai, LI Yueyue, ZHANG Guoqing. Research progress in steroid hormone analysis in vivo by chromatography-mass spectrometry[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(3): 176-180,190. doi: 10.3969/j.issn.1006-0111.2014.03.004
shu

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