Determination of baicalin and cinnamic acid in Bianyan oral solution by HPLC
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摘要: 目的 建立高效液相色谱法同时测定扁咽口服液中黄芩苷和肉桂酸的含量。 方法 色谱柱:Agilent Eclipse XDB-C18(4.6 mm×150 mm,5 μm);流动相:乙腈-2%冰醋酸溶液(23:77);流速:1.0 ml/min;检测波长:280 nm;柱温:室温;进样量:10 μl。 结果 黄芩苷和肉桂酸分别在0.085 1~3.404 8 g(r=0.999 9)和0.020 3~0.811 2 g(r=0.999 9)范围内与其峰面积线性关系良好(r=0.999 9),平均回收率(n=6)为101.68%和101.46%,RSD为285%和2.07%。 结论 本方法快速、简便、准确,能更全面控制和评价扁咽口服液的质量。Abstract: Objective To establish an HPLC method for determination of baicalin and cinnamic acid in Bianyan oral solution simultaneously. Methods The Agilent Eclipse XDB-C18(4.6 mm×150 mm, 5 μm)was used, the mobile phase consisted of ACN-2% acetic acid (23:77), the flow rate was 1.0 ml/min, the detection wavelength was at 280 nm, the column temperature was at room temperature, and the inject volume was 10 μl. Results A good linear correlation was observed within the range of 0.085 1~3.404 8 g for baicalin (r=0.999 9) and 0.020 3~0.811 2 g for cinnamic acid(r=0.999 9). The average recovery (n=6) was 101.68% with RSD 2.85% for baicalin and 101.46% with RSD 2.07% for cinnamic acid. Conclusion The method was rapid, simple and accurate, which could be used for the overall quality control of Bianyan oral solution.
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Key words:
- Bianyan oral solution /
- baicalin /
- cinnamic acid /
- HPLC /
- determination
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